LOCUS       sequence-228703-        1785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express GATA6 in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_GATA6_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             970..999
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 atggccctga ccgacggcgg atggtgtctc cctaaaagat tcggcgccgc tggcgctgat
       61 gcttctgaca gcagagcctt ccccgctagg gaacccagca caccacctag ccccatcagc
      121 agctcaagct ctagctgtag cagaggcgga gagagaggac ctggaggcgc ttctaactgc
      181 ggcacacctc agctggatac agaagccgcc gccggaccac cagccagatc tcttttactt
      241 agcagctacg ccagccaccc ttttggcgct cctcatggac cctctgctcc tggtgtggcc
      301 ggacctggcg gaaacctgag ctcttgggag gaccttctgc tgtttaccga cctggaccag
      361 gctgccaccg ctagcaagct tctgtggagc agcaggggcg ctaagctgag cccttttgcc
      421 cctgagcagc ccgaggagat gtaccagacc ctggctgctt taagctctca gggacctgcc
      481 gcttatgacg gagcccctgg tggatttgtt cactcagcgg cagcagccgc agctgctgca
      541 gccgctgcca gctcacctgt gtatgtgcct accacaagag tgggcagcat gttacctgga
      601 cttccttacc atctgcaggg cagcggaagc ggccctgcta accatgccgg aggagctgga
      661 gctcaccccg gatggcctca ggcttctgca gattctcctc cttatggatc tggaggagga
      721 gcagctggag ggggagctgc aggaccaggt ggagccggaa gcgcagcagc acatgtgtct
      781 gccagatttc cctatagccc tagccctcct atggccaatg gcgctgctag agaacccgga
      841 ggatatgctg cggcaggctc tggcggcgct ggcggagttt ctggaggtgg atcttcactg
      901 gccgctatgg gaggaagaga gcctcagtac tcttctctga gcgccgctag accactgaac
      961 ggcacctatc atcaccacca ccatcaccat catcatcacc ccagccctta ctccccttat
     1021 gtgggagccc cccttacacc cgcttggcct gccggccctt tcgagacacc tgtgctgcac
     1081 agccttcagt ctagagctgg cgcaccttta ccagtgccta gaggcccctc tgccgacttg
     1141 ctggaggatc tgagcgagag cagagagtgc gtgaactgtg gcagcatcca gacacccctg
     1201 tggagaagag acggcaccgg ccactacctg tgcaacgctt gcggcctgta cagcaagatg
     1261 aatgggctga gcagacccct gatcaagccc cagaagaggg tgcccagcag cagacggctg
     1321 ggactgagct gcgccaactg tcataccaca acaaccacac tgtggcggag aaacgccgag
     1381 ggcgagcccg tgtgtaacgc ctgcggcctt tacatgaagc tgcacggcgt gcccagacct
     1441 ctggccatga agaaggaggg aatccagacc agaaagagaa agcccaagaa catcaacaag
     1501 agcaagacct gcagcggcaa cagcaacaac agcatcccca tgacccccac cagcacatct
     1561 agcaacagcg acgactgtag caagaacaca tcacctacca cccagcccac agctagcgga
     1621 gccggcgccc ccgtgatgac aggcgccgga gagtccacaa atcccgagaa tagcgaactg
     1681 aagtactctg gacaggacgg actgtatatc ggcgtgagcc tggcttctcc cgccgaggtg
     1741 accagctctg tcagacctga ctcttggtgt gccctcgccc tggcc
//
