LOCUS       sequence-228712-        1896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express HNF1A in mammalian cells under
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_HNF1A_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1471..1480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtttcta aactgagcca gctgcagacg gagctcctgg cggccctgct ggagtcaggg
       61 ctgagcaaag aggcactgct ccaggcactg ggtgagccgg ggccctacct cctggctgga
      121 gaaggccccc tggacaaggg ggagtcctgc ggcggcggtc gaggggagct ggctgagctg
      181 cccaatgggc tgggggagac tcggggctcc gaggacgaga cggacgacga tggggaagac
      241 ttcacgccac ccatcctcaa agagctggag aacctcagcc ctgaggaggc ggcccaccag
      301 aaagccgtgg tggagaccct tctgcaggag gacccgtggc gtgtggcgaa gatggtcaag
      361 tcctacctgc agcagcacaa catcccacag cgggaggtgg tcgataccac tggcctcaac
      421 cagtcccacc tgtcccaaca cctcaacaag ggcactccca tgaagacgca gaagcgggcc
      481 gccctgtaca cctggtacgt ccgcaagcag cgagaggtgg cgcagcagtt cacccatgca
      541 gggcagggag ggctgattga agagcccaca ggtgatgagc taccaaccaa gaaggggcgg
      601 aggaaccgtt tcaagtgggg cccagcatcc cagcagatcc tgttccaggc ctatgagagg
      661 cagaagaacc ctagcaagga ggagcgagag acgctagtgg aggagtgcaa tagggcggaa
      721 tgcatccaga gaggggtgtc cccatcacag gcacaggggc tgggctccaa cctcgtcacg
      781 gaggtgcgtg tctacaactg gtttgccaac cggcgcaaag aagaagcctt ccggcacaag
      841 ctggccatgg acacgtacag cgggcccccc ccagggccag gcccgggacc tgcgctgccc
      901 gctcacagct cccctggcct gcctccacct gccctctccc ccagtaaggt ccacggtgtg
      961 cgctatggac agcctgcgac cagtgagact gcagaagtac cctcaagcag cggcggtccc
     1021 ttagtgacag tgtctacacc cctccaccaa gtgtccccca cgggcctgga gcccagccac
     1081 agcctgctga gtacagaagc caagctggtc tcagcagctg ggggccccct cccccctgtc
     1141 agcaccctga cagcactgca cagcttggag cagacatccc caggcctcaa ccagcagccc
     1201 cagaacctca tcatggcctc acttcctggg gtcatgacca tcgggcctgg tgagcctgcc
     1261 tccctgggtc ctacgttcac caacacaggt gcctccaccc tggtcatcgg cctggcctcc
     1321 acgcaggcac agagtgtgcc ggtcatcaac agcatgggca gcagcctgac caccctgcag
     1381 cccgtccagt tctcccagcc gctgcacccc tcctaccagc agccgctcat gccacctgtg
     1441 cagagccatg tgacccagag ccccttcatg gccaccatgg ctcagctgca gagcccccac
     1501 gccctctaca gccacaagcc cgaggtggcc cagtacaccc acacaggcct gctcccgcag
     1561 actatgctca tcaccgacac caccaacctg agcgccctgg ccagcctcac gcccaccaag
     1621 caggtcttca cctcagacac tgaggcctcc agtgagtccg ggcttcacac gccggcatct
     1681 caggccacca ccctccacgt ccccagccag gaccctgccg gcatccagca cctgcagccg
     1741 gcccaccggc tcagcgccag ccccacagtg tcctccagca gcctggtgct gtaccagagc
     1801 tcagactcca gcaatggcca gagccacctg ctgccatcca accacagcgt catcgagacc
     1861 ttcatctcca cccagatggc ctcttcctcc cagttg
//
