LOCUS       sequence-228727-         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express HOXA11 in mammalian cells 
            under control of EF1a promoter. Barcoded for transcript detection in
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_HOXA11_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggattttg atgagcgtgg tccctgctcc tctaacatgt atttgccaag ttgtacttac
       61 tacgtctcgg gtccagattt ctccagcctc ccttcttttc tgccccagac cccgtcttcg
      121 cgcccaatga catactccta ctcctccaac ctgccccagg tccaacccgt gcgcgaagtg
      181 accttcagag agtacgccat tgagcccgcc actaaatggc acccccgcgg caatctggcc
      241 cactgctact ccgcggagga gctcgtgcac agagactgcc tgcaggcgcc cagcgcggcc
      301 ggcgtgcctg gcgacgtgct ggccaagagc tcggccaacg tctaccacca ccccaccccc
      361 gcagtctcgt ccaatttcta tagcaccgtg ggcaggaacg gcgtcctgcc acaggctttc
      421 gaccagtttt tcgagacagc ctacggcacc ccggaaaacc tcgcctcctc cgactacccc
      481 ggggacaaga gcgccgagaa ggggcccccg gcggccacgg cgacctccgc ggcggcggcg
      541 gcggctgcaa cgggcgcgcc ggcaacttca agttcggaca gcggcggcgg cggcggctgc
      601 cgggagatgg cggcggcagc agaggagaaa gagcggcggc ggcgccccga gagcagcagc
      661 agccccgagt cgtcttccgg ccacactgag gacaaggccg gcggctccag tggccaacgc
      721 acccgcaaaa agcgctgccc ctataccaag taccagatcc gagagctgga acgggagttc
      781 ttcttcagcg tctacattaa caaagagaag cgcctgcaac tgtcccgcat gctcaacctc
      841 actgatcgtc aagtcaaaat ctggtttcag aacaggagaa tgaaggaaaa aaaaattaac
      901 agagaccgtt tacagtacta ctcagcaaat ccactcctct tg
//
