LOCUS       sequence-228811-        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SOX3 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SOX3_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcgacctg ttcgagagaa ctcatcaggt gcgagaagcc cgcgggttcc tgctgatttg
       61 gcgcggagca ttttgataag cctacccttc ccgccggact cgctggccca caggccccca
      121 agctccgctc cgacggagtc ccagggcctt ttcaccgtgg ccgctccagc cccgggagcg
      181 ccttctcctc ccgccacgct ggcgcacctt cttcccgccc cggcaatgta cagccttctg
      241 gagactgaac tcaagaaccc cgtagggaca cccacacaag cggcgggcac cggcggcccc
      301 gcagccccgg gaggcgcagg caagagtagt gcgaacgcag ccggcggcgc gaactcgggc
      361 ggcggcagca gcggtggtgc gagcggaggt ggcgggggta cagaccagga ccgtgtgaaa
      421 cggcccatga acgccttcat ggtatggtcc cgcgggcagc ggcgcaaaat ggccctggag
      481 aaccccaaga tgcacaattc tgagatcagc aagcgcttgg gcgccgactg gaaactgctg
      541 accgacgccg agaagcgacc attcatcgac gaggccaagc gacttcgcgc cgtgcacatg
      601 aaggagtatc cggactacaa gtaccgaccg cgccgcaaga ccaagacgct gctcaagaaa
      661 gataagtact ccctgcccag cggcctcctg cctcccggtg ccgcggccgc cgccgccgct
      721 gccgcggccg cagccgctgc cgccagcagt ccggtgggcg tgggccagcg cctggacacg
      781 tacacgcacg tgaacggctg ggccaacggc gcgtactcgc tggtgcagga gcagctgggc
      841 tacgcgcagc ccccgagcat gagcagcccg ccgccgccgc ccgcgctgcc gccgatgcac
      901 cgctacgaca tggccggcct gcagtacagc ccaatgatgc cgcccggcgc tcagagctac
      961 atgaacgtcg ctgccgcggc cgccgccgcc tcgggctacg ggggcatggc gccctcagcc
     1021 acagcagccg cggccgccgc ctacgggcag cagcccgcca ccgccgcggc cgcagctgcg
     1081 gccgcagccg ccatgagcct gggccccatg ggctcggtag tgaagtctga gcccagctcg
     1141 ccgccgcccg ccatcgcatc gcactctcag cgcgcgtgcc tcggcgacct gcgcgacatg
     1201 atcagcatgt acctgccacc cggcggggac gcggccgacg ccgcctctcc gctgcccggc
     1261 ggtcgcctgc acggcgtgca ccagcactac cagggcgccg ggactgcagt caacggaacg
     1321 gtgccgctga cccacatc
//
