LOCUS       sequence-228820-         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express SPIC in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_SPIC_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgacgtgtg ttgaacaaga caagctgggt caagcatttg aagatgcttt tgaggttctg
       61 aggcaacatt caactggaga tcttcagtac tcgccagatt acagaaatta cctggcttta
      121 atcaaccatc gtcctcatgt caaaggaaat tccagctgct atggagtgtt gcctacagag
      181 gagcctgtct ataattggag aacggtaatt aacagtgctg cggacttcta ttttgaagga
      241 aatattcatc aatctctgca gaacataact gaaaaccagc tggtacaacc cactcttctc
      301 cagcaaaagg ggggaaaagg caggaagaag ctccgactgt ttgaatacct tcacgaatcc
      361 ctgtataatc cggagatggc atcttgtatt cagtgggtag ataaaaccaa aggcatcttt
      421 cagtttgtat caaaaaacaa agaaaaactt gccgagcttt gggggaaaag aaaaggcaac
      481 aggaagacca tgacttacca gaaaatggcc agggcactca gaaattacgg aagaagtggg
      541 gaaattacca aaatccggag gaagctgact taccagttca gtgaggccat tctccaaaga
      601 ctctctccat cctatttcct ggggaaagag atcttctatt cacagtgtgt tcaacctgat
      661 caagaatatc tcagtttaaa taactggaat gcaaattata attatacata tgccaattac
      721 catgagctaa atcaccatga ttgc
//
