LOCUS       sequence-228832-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Barcoded lentiviral vector to express KLF1 in mammalian cells under 
            control of EF1a promoter. Barcoded for transcript detection in 
            single cell RNA-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
  TITLE     Mapping Cellular Reprogramming via Pooled Overexpression Screens 
            with Paired Fitness and Single-Cell RNA-Sequencing Readout.
  JOURNAL   Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008.
  PUBMED    30448000
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 
            10.1016/j.cels.2018.10.008."
COMMENT     Sequence Label: EF1a_KLF1_P2A_Hygro_Barcode
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcgactg cggagacagc acttccatca atctcaacac tcactgcact ggggccattt
       61 ccagataccc aggacgattt ccttaagtgg tggcggtccg aagaggctca agacatggga
      121 cctggtccgc cggatcccac cgaacctcct ctgcatgtca aaagtgaaga tcagcctggc
      181 gaggaagagg atgacgaaag gggtgccgac gccacttggg acttggatct tctccttacc
      241 aatttctctg gtccggaacc tggcggggca ccacagacgt gcgctctcgc tccctcagaa
      301 gcgagcgggg ctcagtaccc accccctccc gaaactctgg gagcctatgc tgggggtcct
      361 ggactggtgg ctgggttgct tggtagtgag gaccattctg gctgggtacg ccccgctttg
      421 agggcccgcg ctccggacgc ctttgtggga ccggcgctcg ctcctgcacc ggctccggaa
      481 ccaaaagccc tcgcgctgca gcccgtgtac cccggacccg gagccggatc ctcaggggga
      541 tacttcccac ggaccggact cagcgttcca gcggcttccg gggcgccata cggattgttg
      601 agcggctacc cggctatgta tcccgctccc cagtaccaag gacacttcca attgttccgg
      661 ggtcttcaag ggcctgcgcc cgggcctgct accagtccca gtttcctcag ttgtctggga
      721 ccgggaactg ttggcactgg acttggcggg actgcagagg acccaggcgt tatagcagag
      781 acagcgccaa gtaaaagggg ccgacgaagc tgggccagga aacgccaagc tgcgcacact
      841 tgtgcccatc caggttgcgg taaatcctac acgaagagca gtcatcttaa agcacatctt
      901 cgcacacaca cgggcgagaa gccctacgcc tgtacttggg aaggttgcgg ctggagattc
      961 gctagatctg acgagctcac ccggcattat cgaaaacaca ctggccagcg accgttccgg
     1021 tgccaactct gcccaagggc gttcagtcgc tcagatcatc tggctttgca tatgaagcga
     1081 cacctt
//
