LOCUS       sequence-228955-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..57
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             149..175
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
       61 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta ctagaggatc
      121 taccatggca taacgactaa tatttatgta cccatacgat gtgcctgact atgctaagga
      181 caacaccgtg cccctgaagc tgatcgccct gctggccaac ggcgagttcc actctggcga
      241 gcagctggga gagaccctgg gaatgagcag agccgccatc aacaagcaca tccagacact
      301 gagagactgg ggagtggacg tgttcaccgt gcctggcaag ggctacagcc tgcctgagcc
      361 tatccagctg ctgaacgcca agcagatcct gggacagctg gatggcggaa gcgtggccgt
      421 gctgcctgtg atcgactcca ccaatcagta cctgctggac agaatcggag agctgaagtc
      481 cggcgacgcc tgcatcgccg agtaccagca ggctggcaga ggaggcagag gacggaagtg
      541 gttcagccca ttcggagcca acctgtacct gtccatgttc tggagactgg agcagggacc
      601 tgctgctgcc atcggactga gtctggtgat cggaatcgtg atggccgagg tgctgagaaa
      661 gctgggagcc gacaaggtga gagtgaagtg gcctaatgac ctgtacctcc aggaccgcaa
      721 gctggctggc atcctggtgg agctgacagg caagacaggc gatgccgctc agatcgtgat
      781 cggagccgga atcaacatgg ccatgagaag agtggaggag agcgtggtga accagggctg
      841 gatcaccctg caggaggctg gcatcaacct ggaccggaac accctggccg ccatgctgat
      901 c
//
