LOCUS       sequence-228957-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..617
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="TEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKD
                     SDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIR
                     QHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 gaagcgcaga gaagggatcc agcggcggcg gcagcggcgg cggcggcagc acggaatata
       61 agctggtggt ggtgggcgcc ggcggtgtgg gcaagagtgc gctgaccatc cagctgatcc
      121 agaaccattt tgtggacgaa tacgacccca ctatagagga ttcctaccgg aagcaggtgg
      181 tcattgatgg ggagacgtgc ctgttggaca tcctggatac cgccggccag gaggagtaca
      241 gcgccatgcg ggaccagtac atgcgcaccg gggagggctt cctgtgtgtg tttgccatca
      301 acaacaccaa gtcttttgag gacatccacc agtacaggga gcagatcaaa cgggtgaagg
      361 actcggatga cgtgcccatg gtgctggtgg ggaacaagtg tgacctggct gcacgcactg
      421 tggaatctcg gcaggctcag gacctcgccc gaagctacgg catcccctac atcgagacct
      481 cggccaagac ccggcaggga gtggaggatg ccttctacac gttggtgcgt gagatccggc
      541 agcacaagct gcggaagctg aaccctcctg atgagagtgg ccccggctgc atgagctgca
      601 agtgtgtgct ctcctgactc gagtctagag ggcccgacgc gtctggccac cggtcgcccc
//
