LOCUS       sequence-229002-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Kovalski JR, Bhaduri A, Zehnder AM, Neela PH, Che Y, Wozniak GG, 
            Khavari PA
  TITLE     The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2.
  JOURNAL   Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001.
  PUBMED    30639242
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2019 Jan 4. pii: S1097-2765(18)31031-1. doi: 
            10.1016/j.molcel.2018.12.001."
COMMENT     Sequence Label: pLEX-FHH-NRASQ61K/T35A-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..44
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     primer_bind     41..65
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             136..159
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             166..192
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             202..219
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
       61 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactctact
      121 agaggatcta ccatggatta taaagatgac gacgataaag caggttaccc atacgatgtg
      181 cctgactatg ctgcaggttc acatcatcac caccatcatg gatccactga gtacaaactg
      241 gtggtggttg gagcaggtgg tgttgggaaa agcgcactga caatccagct aatccagaac
      301 cactttgtag atgaatatga tcccgccata gaggattctt acagaaaaca agtggttata
      361 gatggtgaaa cctgtttgtt ggacatactg gatacagctg gaaaagaaga gtacagtgcc
      421 atgagagacc aatacatgag gacaggcgaa ggcttcctct gtgtatttgc catcaataat
      481 agcaagtcat ttgcggatat taacctctac agggagcaga ttaagcgagt aaaagactcg
      541 gatgatgtac ctatggtgct agtgggaaac aagtgtgatt tgccaacaag gacagttgat
      601 acaaaacaag cccacgaact ggccaagagt tacgggattc cattcattga aacctcagcc
      661 aagaccagac agggtgttga agatgctttt tacacactgg taagagaaat acgccagtac
      721 cgaatgaaaa aactcaacag cagtgatgat gggactcagg gttgtatggg attgccatgt
      781 gtggtgatgt aactcgagtc tagagggccc gacgcgtctg gccaccggtc gcccctctcc
      841 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
      901 ctatatgtta ttttcc
//
