LOCUS       sequence-229047-        4642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4642)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FL
FEATURES             Location/Qualifiers
     source          1..4642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        107..464
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        107..298
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(130..150)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      315..450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      494..503
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             500..2152
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2196..2317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2207..2226)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2261..2280
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2565..2582)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2736..3324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2816..2835)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3524..4315,4316..4384))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4489..4537
                     /note="synthetic polyadenylation signal"
     misc_feature    4551..4642
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4591..4610
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tgcgcagcac
       61 catggcctga aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga
      121 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
      181 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
      241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      301 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      361 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
      421 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt ggcaatccgg
      481 tactgttggt aaagccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt
      541 ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc
      601 cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc
      661 cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac
      721 aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg
      781 tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct
      841 gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca
      901 aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag
      961 caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc
     1021 cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct
     1081 gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac
     1141 cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga
     1201 caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg
     1261 ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt
     1321 gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt
     1381 cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag
     1441 cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc
     1501 aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga
     1561 aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt
     1621 ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg
     1681 ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa
     1741 ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat
     1801 cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact
     1861 ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga
     1921 cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac
     1981 cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg
     2041 tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa
     2101 gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt aataattcta
     2161 gagtcggggc ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     2221 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2281 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2341 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2401 atgtggtaaa atcgataagg atccgtcgac cgatgccctt gagagccttc aacccagtca
     2461 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     2521 tcatgcaact cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg
     2581 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2641 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2701 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2761 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2821 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2881 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2941 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3001 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3061 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3121 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3181 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3241 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3301 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3361 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3421 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3481 acttggtctg acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca
     3541 gtgaggcacc gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg
     3601 tcgtgtagat cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc
     3661 cgcgagagcc gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg
     3721 ccgagcgaag aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc
     3781 gtgatgctag agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta
     3841 ctggcatcgt ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc
     3901 ggtcaagccg ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc
     3961 ctccgatcgt tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac
     4021 tacacaattc tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact
     4081 caaccaagtc gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta
     4141 tacgggacaa caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt
     4201 cttcggggcg gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca
     4261 ctcttgcacc cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa
     4321 aaacaggcaa gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc
     4381 tcatactcgt cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc
     4441 aaggataagt aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct
     4501 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
     4561 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
     4621 gcaggtgcca gaacatttct ct
//
