LOCUS       sequence-229155-        7911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_060570-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3075..3092
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3144..3199
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3198..3217)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5435..5453)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5521..5625
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5626..5694,5695..6486)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5844..5863)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6657..7245
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7146..7165
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7399..7416
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7533..7554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7569..7574,7575..7592,7593..7599)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7607..7623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7612..7634
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7631..7647
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7631..7647
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcct
     1201 acaaccccat gagcgagaag ctggaaagcc tgctgctgag cctggacaag ctggtgtact
     1261 actacgagca gcacgtgtcc gagttcaact tcgacggcat cttcggcctg atccccctga
     1321 agggcgccct ggaatccatc accgacgaca gcctgtacga gaactacgag agagtgaaga
     1381 tcgccctgct gcccctgatg cggaagctga aaacccagat catgcggatc aacgtgatca
     1441 tccggaagtc cgtgaagttc ttcaagaagg cccgggacag ctactacacc aagatgggcc
     1501 ggctgctggc cctgaagtgg aacttccccg gccagaacac catgtacagc tacctgagcg
     1561 accccgagcc catcagcagc gcccagaaca tcagctccga cgagtgcatc atccagctgc
     1621 tgaacaccaa caactacggc accctggact gccacctgag catggaatgc atcctgaagc
     1681 tgatggactt caacagccgg ggctacgagc tggcccacca ggtgctgtac atcctgatca
     1741 gccaccaggt caactgcacc gacagcctgg acggcatgct gagcaacctg agcaccagcg
     1801 tgggcagcct gcagaacaag ctgtgcagca tcctgtaccg ggacttcgtg aacctgttcc
     1861 tgagcagcga gacaaccccc cggaaccggg acatcctgct ggaagagatg ttcgtgtgcg
     1921 gcagcatcgg ctacgagaac ttcatccagt tcagcatcct gagccgggtg ctgagctggc
     1981 agcagcccag cggctgcttc atctccagcg gcagcaccga cggcaagagc aagcacaaga
     2041 acaccaagat catgttccac gagcggcacc ccctggtgga acacagacac gccgatggct
     2101 gcctgagcca catgacctct gtggccgctg ccgtgatggg cctgtacttc agagccttct
     2161 tcatccccac cggctacgtg gacggctacc tgtccgccag catgcctatt atctggaagg
     2221 tgttcctcgt gcggagctac accagcctgc ccaagctgat cgaggacgac aacaaccagg
     2281 acggcaacag caaccaggac atcttcaacg ccttcaccaa ccggctgatc cacgtgaagg
     2341 gcagcaccta cctgcccagc ggcatcccct tcagcctgag ctacagcacc cacttcaagg
     2401 ataagctgga cctgatcaac tctggcgcgc cgtcgacctc catcacggcc tataagagtg
     2461 agggggagtc agcggagttc tccttcccac tcaaccttgg agaggaaagc ctgcagggag
     2521 agttgagatg gaaggcagag aaggctcctt cttcccagtc ctggatcacc ttctccctaa
     2581 agaaccaaaa ggtgtctgtg cagaagtcta ctagcaaccc caagttccag ctgtccgaaa
     2641 cgctcccact cacccttcag ataccccagg tctcccttca gtttgctggt tctggcaacc
     2701 tgaccctgac tctggacaga gggatactgt atcaggaagt gaacctggtg gtgatgaaag
     2761 tgactcagcc cgacagcaac actttgacct gtgaggtgat gggacccacc tcacccaaga
     2821 tgagactgat cttgaagcag gagaatcagg aggccagggt ctccaggcag gagaaagtga
     2881 ttcaagtgca ggcccctgaa gcaggggtgt ggcaatgtct actgagtgaa ggtgaagagg
     2941 tcaagatgga ctccaagatc caggttttat ccaaagggtt gaattccggc agcctgcacc
     3001 acatcctgga cgcccagaag atgctgtgga accacaggga caggaacctg cctcctctgg
     3061 cccctctggg cccccaccac catcaccacc attgagtcta gacacgtgat taattaagga
     3121 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     3181 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     3241 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     3301 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3361 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3421 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3481 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3541 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3601 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3661 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     3721 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     3781 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     3841 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     3901 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     3961 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     4021 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     4081 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     4141 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     4201 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     4261 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     4321 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4381 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4441 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4501 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4561 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4621 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4681 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     4741 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     4801 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     4861 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4921 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     4981 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     5041 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     5101 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     5161 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     5221 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     5281 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5341 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5401 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5461 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5521 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5581 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5641 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5701 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5761 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5821 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     5881 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5941 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6001 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6061 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6121 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     6181 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6241 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6301 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6361 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6421 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6481 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6541 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6601 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6661 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6721 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6781 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6841 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6901 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6961 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7021 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7081 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7141 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7201 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7261 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7321 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7381 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7441 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7501 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7561 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7621 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7681 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     7741 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     7801 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     7861 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg c
//
