LOCUS       sequence-229167-        9465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9465)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 9465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_176540-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..9465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4629..4646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4698..4753
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4752..4771)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6989..7007)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7075..7179
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7180..7248,7249..8040)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7398..7417)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8211..8799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8700..8719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8953..8970
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9087..9108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9123..9128,9129..9146,9147..9153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9161..9177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9166..9188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9185..9201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9185..9201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 tggacgagat cgagctgcgg ctgcggatgc gggaagagat cgcccccagc acccggatcg
     1261 gcagcgtgat cagcagcctg cccgtgaacc tgcggagcca gatgcacttc ctgaccgaca
     1321 gcaccttctt caccgtgacc cccaccaccg gcgagatcag agtgggccgg ctgatcgacc
     1381 gcgagcggct gtgccctgag ttcaagctgt gctgcggcgt gatcacatgc gagctgaagg
     1441 ccagcatctt cgtgaccaac aaggccgctg gcgagttcgc cgccagcgtg ctgctgaagg
     1501 tggaaatcca ggaccagaac gacaaccggc ccaccttcag cgcccagagc cagaccgtga
     1561 ccctgagcga ggccaccacc gtgggcaccc tgatcagcat caacgccgcc accgacgccg
     1621 acatcgaccc cgagaaccag atccagcggt acaccctggt ggaacacacc ggcaccttca
     1681 agctggacct gagcggcctg cccaccgtgc ggctggaact gctcagaccc ctggaccggg
     1741 aacaggtgtc cgagtacaag gccagcctgg aagcctgcga ccctggcgcc tgtacccggc
     1801 agaccctgga aattcagatc ctggacgaga acgataacag ccccagactg gccaacaccc
     1861 ggttcaacaa gagactgatc gagaacatcc ctgtgggcac cgtggtgctg cagctgaacg
     1921 ccaccgacat cgatagcggc gagcggggca gagtgatgta cagcttccac ggcaacatcg
     1981 acagcgacct gaccgagaca ttcgaactcg tgcgggacac aggcgagatc aagctgagaa
     2041 ggcccctggc cgccaacatc cgggacagct acgagttcaa agtgatcgcc tgcgacgccg
     2101 tgaaccgggc ctgcagcggc aacgagaaca gcaccgccga catcaccttc accgtggaag
     2161 atgtgaacaa cttccccccc tccatccacg ccgtggctgc tggcgctgcc ctggtcacaa
     2221 gcagcagcga ccagaacaaa gtgggcgaga gcaagaccgg cgagagcgac cacaacagct
     2281 tcaacgagcc caagcccggc gacgagctga gcatcctgga aaacagcccc cccagccaga
     2341 tcgccgtgct gaccgtgcgg gacgacgaca ccggcgacaa cagcaaggtg tcctgctccc
     2401 tgaacgacac caacagcaac atcctgagca acggcgagaa aaccacccag aacttcatcc
     2461 tgacccctag cacccccggc atctacagcc tgcggaccgc caaggtgttc gactacgaga
     2521 gcgagagcac catccggacc accgtcgtgt gccacgacta cggcaagccc cagcagctga
     2581 ccagcgccag agtgatcacc gtgcagatca tcgacgtgaa cgagttccag cccgagttca
     2641 gcagacgggt gtacgtgtcc ggcgtgcccg agaacgcccc tcccggcatg gatatcctga
     2701 ccaccaccgc cctggacaga gacagagccg ccgtgctgtc ctacaagttc gccccctcca
     2761 gcaccctgag cttcagcgag ggcgagaagg acagccacaa gagcgacagc atccagatcg
     2821 acgacgaagt gaacaagcac ttcatcatcg agtcccagac cggcatcatc cggacctccc
     2881 agatccccct ggatcgcgag aagatcggct ctgtgaccct ggtggtgctg gtcaccgaca
     2941 gcgtgtcccc ccccatcttc accgccacca ccacagtgtc catcgagatt ctggatgaga
     3001 atgacaacgc ccccgtgttc accaaccccc ccgtgcggtc tagcgaggcc ccccagggcg
     3061 acaacgagaa gttcgccttc accatcatgg aaaatgcccc cagattcacc cagctgagcc
     3121 agcggctgga agccaaggac cccgaccagg gcgataacgg ccgggtgcag tttagcctgc
     3181 tggccaccta cgccctgacc aagagcagcc ggcggaacca gttcatcggc agcagcccca
     3241 atatgctgac cagcttcgag cactttatcg acagacccgg ctccgacctg aacagcgagg
     3301 ccggcatgac ccggcacgtg gtggaccagc ccatcttccg gatcaccagc aacggcgtga
     3361 tcgagacact ggtggaactg gaccgcgaga cagtgcccgt gtacatcctg aagatcggcg
     3421 tgcgcgacag aggcgcccag cctctggcct ccaccaccat gctgagagtg aacgtgcagg
     3481 acgccaacga caatgcccct gtgtgggtgt tccccacccc cgtggaccgg accatcaacc
     3541 tgaccaccgg catgaagccc ggcagcctgg ccggcagact gagagccgag gacgccgata
     3601 gctttgaggc cggcaaggtg gaatacctgt tcctgggccc cagaggcgag acaatcgagg
     3661 gcgtgaccct ggaagagggc ctgctgaacg tgctgaaccc ccccaagcct ggcctgacaa
     3721 agaccgtgaa gcctggccag aagctgagca gcaacagcga cgacctgaag ggctacagag
     3781 ccggccctct gtacctgaac ggcacaaccg gggagatctg ggtggcccag gccctgacca
     3841 gcggcaccat taacctgcac ctgagggccg aggatcaggg cacccccaga tcccacaccg
     3901 acgcctggct gaccatcaac gtgttcatcg accccagcga gggcatcggc tttctgagct
     3961 tcggcggcga tggcactggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     4021 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     4081 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     4141 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     4201 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     4261 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     4321 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     4381 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     4441 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     4501 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggcagcctg caccacatcc
     4561 tggacgccca gaagatgctg tggaaccaca gggacaggaa cctgcctcct ctggcccctc
     4621 tgggccccca ccaccatcac caccattgag tctagacacg tgattaatta aggatccccc
     4681 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     4741 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     4801 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     4861 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     4921 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     4981 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     5041 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     5101 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     5161 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     5221 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     5281 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     5341 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     5401 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     5461 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     5521 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     5581 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     5641 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     5701 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     5761 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     5821 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     5881 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     5941 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     6001 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     6061 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     6121 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     6181 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     6241 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     6301 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     6361 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     6421 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     6481 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     6541 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     6601 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     6661 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     6721 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     6781 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     6841 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     6901 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     6961 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     7021 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7081 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7141 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7201 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7261 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7321 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7381 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     7441 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7501 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7561 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7621 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7681 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     7741 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7801 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7861 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7921 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7981 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8041 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8101 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8341 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8881 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8941 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9001 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     9061 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     9121 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     9181 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     9241 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     9301 agaactggta ggtatggaag atctatacat tgaatcaata ttggcaatta gccatattag
     9361 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
     9421 atcataatat gtacatttat attggctcat gtccaatatg accgc
//
