LOCUS       sequence-229175-        7074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_194840-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1524..1535
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2238..2255
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2307..2362
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2361..2380)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4598..4616)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4684..4788
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4789..4857,4858..5649)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5007..5026)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5820..6408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6309..6328
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6562..6579
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6696..6717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6732..6737,6738..6755,6756..6762)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6770..6786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6775..6797
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6794..6810
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6794..6810
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 agcctttcag agactgcggc agcaagttcg gcaagctgta cagcctgacc gtgaccccct
     1261 gcgacagaac cccctgcgcc ctgtacaagg gccagaacgc caccatcacc atcgagttca
     1321 caacccagga aaccgtcaag gacggccaca tcagcgtgca cggcgtgatc gcccacgtgc
     1381 ccatcccttt cgccctggac aacagcaacc tgtgcgagtt cgtgtcccct acctgccccc
     1441 tgatccccag catcggcaag tacacccaca cctacagcct gtacgtgaag gccatgtacc
     1501 ccagcatcag cctgaccatc agatgggagc tgcaggacag cagcaacgag gacatcgtgt
     1561 gcgtggaatt ccccgtgcag ctgattggcg cgccgtcgac ctccatcacg gcctataaga
     1621 gtgaggggga gtcagcggag ttctccttcc cactcaacct tggagaggaa agcctgcagg
     1681 gagagttgag atggaaggca gagaaggctc cttcttccca gtcctggatc accttctccc
     1741 taaagaacca aaaggtgtct gtgcagaagt ctactagcaa ccccaagttc cagctgtccg
     1801 aaacgctccc actcaccctt cagatacccc aggtctccct tcagtttgct ggttctggca
     1861 acctgaccct gactctggac agagggatac tgtatcagga agtgaacctg gtggtgatga
     1921 aagtgactca gcccgacagc aacactttga cctgtgaggt gatgggaccc acctcaccca
     1981 agatgagact gatcttgaag caggagaatc aggaggccag ggtctccagg caggagaaag
     2041 tgattcaagt gcaggcccct gaagcagggg tgtggcaatg tctactgagt gaaggtgaag
     2101 aggtcaagat ggactccaag atccaggttt tatccaaagg gttgaattcc ggcagcctgc
     2161 accacatcct ggacgcccag aagatgctgt ggaaccacag ggacaggaac ctgcctcctc
     2221 tggcccctct gggcccccac caccatcacc accattgagt ctagacacgt gattaattaa
     2281 ggatcccccg acctcgacct ctggctaata aaggaaattt attttcattg caatagtgtg
     2341 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttggtcga
     2401 gatccctcgg agatctctag ctagaggatc gatccccgcc ccggacgaac taaacctgac
     2461 tacgacatct ctgccccttc ttcgcggggc agtgcatgta atcccttcag ttggttggta
     2521 caacttgcca actgggccct gttccacatg tgacacgggg ggggaccaaa cacaaagggg
     2581 ttctctgact gtagttgaca tccttataaa tggatgtgca catttgccaa cactgagtgg
     2641 ctttcatcct ggagcagact ttgcagtctg tggactgcaa cacaacattg cctttatgtg
     2701 taactcttgg ctgaagctct tacaccaatg ctgggggaca tgtacctccc aggggcccag
     2761 gaagactacg ggaggctaca ccaacgtcaa tcagaggggc ctgtgtagct accgataagc
     2821 ggaccctcaa gagggcatta gcaatagtgt ttataaggcc cccttgttaa ccctaaacgg
     2881 gtagcatatg cttcccgggt agtagtatat actatccaga ctaaccctaa ttcaatagca
     2941 tatgttaccc aacgggaagc atatgctatc gaattagggt tagtaaaagg gtcctaagga
     3001 acagcgatat ctcccacccc atgagctgtc acggttttat ttacatgggg tcaggattcc
     3061 acgagggtag tgaaccattt tagtcacaag ggcagtggct gaagatcaag gagcgggcag
     3121 tgaactctcc tgaatcttcg cctgcttctt cattctcctt cgtttagcta atagaataac
     3181 tgctgagttg tgaacagtaa ggtgtatgtg aggtgctcga aaacaaggtt tcaggtgacg
     3241 cccccagaat aaaatttgga cggggggttc agtggtggca ttgtgctatg acaccaatat
     3301 aaccctcaca aaccccttgg gcaataaata ctagtgtagg aatgaaacat tctgaatatc
     3361 tttaacaata gaaatccatg gggtggggac aagccgtaaa gactggatgt ccatctcaca
     3421 cgaatttatg gctatgggca acacataatc ctagtgcaat atgatactgg ggttattaag
     3481 atgtgtccca ggcagggacc aagacaggtg aaccatgttg ttacactcta tttgtaacaa
     3541 ggggaaagag agtggacgcc gacagcagcg gactccactg gttgtctcta acacccccga
     3601 aaattaaacg gggctccacg ccaatggggc ccataaacaa agacaagtgg ccactctttt
     3661 ttttgaaatt gtggagtggg ggcacgcgtc agcccccaca cgccgccctg cggttttgga
     3721 ctgtaaaata agggtgtaat aacttggctg attgtaaccc cgctaaccac tgcggtcaaa
     3781 ccacttgccc acaaaaccac taatggcacc ccggggaata cctgcataag taggtgggcg
     3841 ggccaagata ggggcgcgat tgctgcgatc tggaggacaa attacacaca cttgcgcctg
     3901 agcgccaagc acagggttgt tggtcctcat attcacgagg tcgctgagag cacggtgggc
     3961 taatgttgcc atgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat
     4021 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     4081 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     4141 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     4201 ctgtatccgg gtagcatatg ctatcctaat agagattagg gtagtatatg ctatcctaat
     4261 ttatatctgg gtagcatata ctacccaaat atctggatag catatgctat cctaatctat
     4321 atctgggtag catatgctat cctaatctat atctgggtag cataggctat cctaatctat
     4381 atctgggtag catatgctat cctaatctat atctgggtag tatatgctat cctaatttat
     4441 atctgggtag cataggctat cctaatctat atctgggtag catatgctat cctaatctat
     4501 atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat cctcatgata
     4561 agctgtcaaa catgagaatt ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     4621 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     4681 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4741 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4801 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4861 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4921 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4981 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     5041 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5101 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5161 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5221 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5281 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     5341 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5401 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5461 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5521 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5581 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5641 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5701 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5761 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5821 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5881 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5941 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6001 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6061 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6121 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6181 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6241 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6301 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6361 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6421 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6481 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6541 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6601 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6661 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6721 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6781 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta gctagaggtc
     6841 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     6901 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     6961 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     7021 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgc
//
