LOCUS       sequence-22923-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V28 pCD-6FLAGtag
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(66..89)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     5'UTR           complement(103..145)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     intron          181..277
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(316..645)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      330..465
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     630..650
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtttngttgg actcgtatac gactcactat agttctagag gctcgagagg ccttgaattc
       61 gagatcttat cgtcgtcatc cttgtaatcc atggtggatc tgccaaagtt gagcgtttat
      121 tctgagcttc tgcaaaaaga acaagcaagc ttagagcaga agtaacactt ccgtacaggc
      181 ctagaagtaa aggcaacatc cactgaggag cagttctttg atttgcacca ccaccggatc
      241 cgggacctga aataaaagac aaaaagacta aacttaccag ttaactttct ggtttttcag
      301 ttcctcgagg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
      361 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
      421 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
      481 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
      541 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
      601 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagccaagc
      661 ttatcgatga taagctgtca acatgagaat tggtcgactg gcgtatagcg aaaggccgcc
//
