LOCUS       sequence-229237-        7401 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_123550-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7401
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2565..2582
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2634..2689
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2688..2707)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4925..4943)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5011..5115
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5116..5184,5185..5976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5334..5353)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6147..6735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6636..6655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6889..6906
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7023..7044
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7059..7064,7065..7082,7083..7089)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7097..7113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7102..7124
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7121..7137
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7121..7137
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccc
     1201 agcctaagga cgcccagctg agaaagctgc tgcagtacca caacgagctg cggcggaacc
     1261 tgaccgcctg caagctggaa ggccagcccc ctgccaagaa cctgctggac ctgaagtggg
     1321 acaatgagct ggccagcaag gccaaggacc tggccaacga gtgctacttc caccacaacg
     1381 acgtggacct gcccgagaag tggcagtaca tcggccagaa tatcgccggc taccagacca
     1441 tcgagcaggc cttcgacgcc tggaaggacg agtacaagca gtacaactac tacagcaaga
     1501 gctgcagcgg cgtgtgcggc cactacaccc agctcgtgtg gcagaacacc acccacgtgg
     1561 gctgcggcat caccaactgt accggcagct acagcttccc ctacggcctg agcgtcgtgt
     1621 gcaactacgg ccctggcggc aactacgagg gcagataccc ctacgaggcc aagagccagg
     1681 atgagtgcta cgccaccacc accaagcggc ccaccaccac aaaaagaccc acaaccacta
     1741 agaggcccag cacaactacc accaagaggc ctggcaccac ccccacccag aaacccggcg
     1801 tgcccaagca gatccccaag cccatctggc ccagcatcat cagcacctgg aacgagtacg
     1861 ccaccagcaa catgatccag ggcatcgtga cccagacctg catctgcgtg aaaggcgcgc
     1921 cgtcgacctc catcacggcc tataagagtg agggggagtc agcggagttc tccttcccac
     1981 tcaaccttgg agaggaaagc ctgcagggag agttgagatg gaaggcagag aaggctcctt
     2041 cttcccagtc ctggatcacc ttctccctaa agaaccaaaa ggtgtctgtg cagaagtcta
     2101 ctagcaaccc caagttccag ctgtccgaaa cgctcccact cacccttcag ataccccagg
     2161 tctcccttca gtttgctggt tctggcaacc tgaccctgac tctggacaga gggatactgt
     2221 atcaggaagt gaacctggtg gtgatgaaag tgactcagcc cgacagcaac actttgacct
     2281 gtgaggtgat gggacccacc tcacccaaga tgagactgat cttgaagcag gagaatcagg
     2341 aggccagggt ctccaggcag gagaaagtga ttcaagtgca ggcccctgaa gcaggggtgt
     2401 ggcaatgtct actgagtgaa ggtgaagagg tcaagatgga ctccaagatc caggttttat
     2461 ccaaagggtt gaattccggc agcctgcacc acatcctgga cgcccagaag atgctgtgga
     2521 accacaggga caggaacctg cctcctctgg cccctctggg cccccaccac catcaccacc
     2581 attgagtcta gacacgtgat taattaagga tcccccgacc tcgacctctg gctaataaag
     2641 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     2701 atatgggagg gcaaatcatt tggtcgagat ccctcggaga tctctagcta gaggatcgat
     2761 ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc gcggggcagt
     2821 gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt ccacatgtga
     2881 cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc ttataaatgg
     2941 atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     3001 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     3061 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     3121 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     3181 taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt agtatatact
     3241 atccagacta accctaattc aatagcatat gttacccaac gggaagcata tgctatcgaa
     3301 ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg agctgtcacg
     3361 gttttattta catggggtca ggattccacg agggtagtga accattttag tcacaagggc
     3421 agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct gcttcttcat
     3481 tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt gtatgtgagg
     3541 tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg ggggttcagt
     3601 ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca ataaatacta
     3661 gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg tggggacaag
     3721 ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca cataatccta
     3781 gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag acaggtgaac
     3841 catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac agcagcggac
     3901 tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca atggggccca
     3961 taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc acgcgtcagc
     4021 ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac ttggctgatt
     4081 gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa tggcaccccg
     4141 gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc tgcgatctgg
     4201 aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg tcctcatatt
     4261 cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat actacccaaa
     4321 tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     4381 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     4441 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     4501 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctaataga
     4561 gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta cccaaatatc
     4621 tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4681 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     4741 tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc
     4801 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc
     4861 cgggtagcat atgctatcct catgataagc tgtcaaacat gagaattttc ttgaagacga
     4921 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4981 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5041 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5101 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5161 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5221 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5281 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5341 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     5401 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5461 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5521 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5581 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5641 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5701 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5761 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5821 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5881 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5941 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6001 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6061 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6481 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6541 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6601 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6661 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6721 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6781 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6841 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6901 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6961 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     7021 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     7081 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     7141 gattacgcca agctctagct agaggtcgac caattctcat gtttgacagc ttatcatcgc
     7201 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     7261 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     7321 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     7381 gctcatgtcc aatatgaccg c
//
