LOCUS       sequence-229238-        6957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_181070-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2121..2138
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2190..2245
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2244..2263)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4481..4499)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4567..4671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4672..4740,4741..5532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4890..4909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5703..6291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6192..6211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6445..6462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6579..6600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6615..6620,6621..6638,6639..6645)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6653..6669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6658..6680
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6677..6693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6677..6693
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgcca
     1201 agcccagcct ggaaatcgac gccaacgaca tccagtgcga catctgcatg ggcgtggtgt
     1261 ccctgggcaa gctgttcctg gtgtccccca tcatggacag catcaagaaa atgctggtgg
     1321 aaaagctgtg caccctgccc ggcatcatgc ggcggagatg catgcactgg ggctacgacc
     1381 agatcgacga gatggtggtg cacttcctga agcagaagtc cagcaagccc tgcaagtact
     1441 tcctgctgtg cagcaccaac accagccagg gcgcgccgtc gacctccatc acggcctata
     1501 agagtgaggg ggagtcagcg gagttctcct tcccactcaa ccttggagag gaaagcctgc
     1561 agggagagtt gagatggaag gcagagaagg ctccttcttc ccagtcctgg atcaccttct
     1621 ccctaaagaa ccaaaaggtg tctgtgcaga agtctactag caaccccaag ttccagctgt
     1681 ccgaaacgct cccactcacc cttcagatac cccaggtctc ccttcagttt gctggttctg
     1741 gcaacctgac cctgactctg gacagaggga tactgtatca ggaagtgaac ctggtggtga
     1801 tgaaagtgac tcagcccgac agcaacactt tgacctgtga ggtgatggga cccacctcac
     1861 ccaagatgag actgatcttg aagcaggaga atcaggaggc cagggtctcc aggcaggaga
     1921 aagtgattca agtgcaggcc cctgaagcag gggtgtggca atgtctactg agtgaaggtg
     1981 aagaggtcaa gatggactcc aagatccagg ttttatccaa agggttgaat tccggcagcc
     2041 tgcaccacat cctggacgcc cagaagatgc tgtggaacca cagggacagg aacctgcctc
     2101 ctctggcccc tctgggcccc caccaccatc accaccattg agtctagaca cgtgattaat
     2161 taaggatccc ccgacctcga cctctggcta ataaaggaaa tttattttca ttgcaatagt
     2221 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttggt
     2281 cgagatccct cggagatctc tagctagagg atcgatcccc gccccggacg aactaaacct
     2341 gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg
     2401 gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc aaacacaaag
     2461 gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc caacactgag
     2521 tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca ttgcctttat
     2581 gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct cccaggggcc
     2641 caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta gctaccgata
     2701 agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt taaccctaaa
     2761 cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc taattcaata
     2821 gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa agggtcctaa
     2881 ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg gggtcaggat
     2941 tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc aaggagcggg
     3001 cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag ctaatagaat
     3061 aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg
     3121 acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct atgacaccaa
     3181 tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa cattctgaat
     3241 atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga tgtccatctc
     3301 acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac tggggttatt
     3361 aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact ctatttgtaa
     3421 caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct ctaacacccc
     3481 cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag tggccactct
     3541 tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc ctgcggtttt
     3601 ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac cactgcggtc
     3661 aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat aagtaggtgg
     3721 gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac acacttgcgc
     3781 ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga gagcacggtg
     3841 ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat atgctatcct
     3901 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     3961 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     4021 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     4081 aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat atgctatcct
     4141 aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc tatcctaatc
     4201 tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     4261 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     4321 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     4381 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctcatg
     4441 ataagctgtc aaacatgaga attttcttga agacgaaagg gcctcgtgat acgcctattt
     4501 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     4561 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4621 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4681 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4741 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4801 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4861 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     4921 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4981 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5041 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5101 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     5161 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     5221 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     5281 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     5341 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5401 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5461 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5521 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6481 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6541 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6601 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6661 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct ctagctagag
     6721 gtcgaccaat tctcatgttt gacagcttat catcgcagat ccgggcaacg ttgttgccat
     6781 tgctgcaggc gcagaactgg taggtatgga agatctatac attgaatcaa tattggcaat
     6841 tagccatatt agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata
     6901 cgttgtatct atatcataat atgtacattt atattggctc atgtccaata tgaccgc
//
