LOCUS       sequence-229400-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Part of GoldenGate Yarrowia lipolytica toolkit for assembling an 
            expression plasmid for Y. lipolytic yeast. Sequence for homologous 
            integration into Y. lipolytica genome. Linearization of cassette by 
            NotI restriction enzyme after the assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Larroude M, Park YK, Soudier P, Kubiak M, Nicaud JM, Rossignol T
  TITLE     A modular Golden Gate toolkit for Yarrowia lipolytica synthetic 
            biology.
  JOURNAL   Microb Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427.
  PUBMED    31148366
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 May 31. doi: 10.1111/1751-7915.13427."
COMMENT     Sequence Label: GGE253
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtctctgcc tggcctgatc cgagccatgg cagtcatatt cgaggcatag ctgtgaagac
       61 atgtacgagg acaatgcgaa atttgcatct gtatccacat ccgggctctt tgagagcaac
      121 gcacgctcag tatttctaca cgtacatgca caatcgtcga cataaactac tgtactctga
      181 agatcaccgc aagcaaatgt cactagctac agctacgagt gagataccac cacagggacg
      241 tggaatatgc gactccgatc cactcccgct aaaacaatgt gtccaattat tccgaaacct
      301 gcttttggac agctgatggc ccgcacgttg catctacttt ttgccgcggc ctaccccggc
      361 agcagcttgc attaatccgt gctgggctgg tgggatcggg tcgaatggga gtgaccaaga
      421 caagagatgc aaacgggtcc aagtctccgg gtgaataatg gggccatgtg tcgggttggg
      481 gctgacttga cctggatttg gagcgaccaa cgaggccaaa atcaacgtga atcgcgccca
      541 cagagcccac aatatccctg ggtcgtgtac gtacgataca atactagagc cactagcgtc
      601 aacttaagtc tcgagtttgt gcggggaagt cacaccatag tgcagaatga ccaaggagcg
      661 gtacgcacgt taccaacact ataagatcga ctagtctgga agaggtggaa gtgaataacg
      721 aacacagagg ggaaatacga tggagatatt cccctcccct gggcctgtag aatatggcta
      781 tgggtacagg acgtgcatag tcctcgcact ccacacaaga cccatcaggc ccccaacagg
      841 cagcccaaac tttgagccac ataggttgaa actctacctg tcaaagtgac tgtacttgaa
      901 cactgcgagt ttagccttaa tagtgaggtt tgttcccatt gctccgctat tgacaccaca
      961 acccccaact cttcctcttt ctctctcgca acaaccgatt ccaacaggta gagacc
//
