LOCUS       sequence-229423-       11267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in
            zebrafish melanocytes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell 
            CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I
  TITLE     Human tumor genomics and zebrafish modeling identify SPRED1 loss as 
            a driver of mucosal melanoma.
  JOURNAL   Science. 2018 Nov 1. pii: science.aau6509. doi: 
            10.1126/science.aau6509.
  PUBMED    30385465
REFERENCE   2  (bases 1 to 11267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509."
COMMENT     Sequence Label: MiniCoopR mitfa:NRAS
FEATURES             Location/Qualifiers
     source          1..11267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(514..525,526..688)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2991..3015
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3589..3613)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3615..3749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3812..3832)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3840..3856)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3872..4006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(8797..8984,8985..8996)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9030..9046)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9030..9046)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9083..9103)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9083..9101)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9122..9138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9135..9157)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9146..9162
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9170..9176,9177..9194,9195..9200))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9215..9236
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9353..9370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9524..10112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9604..9623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10283..11074,11075..11143))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10906..10925
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11144..11248)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttgtga gtgctaacaa ctgggctgct
     1141 gtgccgctct ttcgaggaaa tgaaaggaac tctggcggcc tatgatgagt taggaattgt
     1201 ctgattggtg aatcatacat aggctaatgt gggaccagct gtagtcagtc ataagcacgt
     1261 gattctctcg aaattagttt ataaataaac ttcactaaac tccaaccgtt cattctgttt
     1321 tagatgttgc ctttaaactt gccttaagat gttaataaag ttgctttttg gttgttgaca
     1381 aagctaggca taggacgata accgttttca aggtataccc cggtttgaaa aagtcaaggg
     1441 tttaaaacca ccaatatttt ctgctatacc gttcctaagg tatatttaag attttttatt
     1501 tattttttat ttttttgttt tttaggacaa cagcattttc agcagaaaag agatacaaaa
     1561 atgcttttta aattgtaaag aaatctgttt ttgaaactaa tgaagacagc agaagtcgat
     1621 cattcattga aattattaag cttgacatgt tttctgttcc aaaatatttt ttcaatgttt
     1681 ctcaaaataa aatatattat gtttaaagtt tttgtttttt actaagacat taaaaaaaat
     1741 aatatatttt agagtggcaa tcacaatacc atcaaactgt gatattttta tcaaaggtta
     1801 tcataccatc agaatcttat accccatgcc tagacacagc tgttctctct atctctctat
     1861 gttaatgcag tttccactcc attgacgaga tggttctctg taccacatac tcatccccat
     1921 tttacaatag ctacatttgc tcaagatgta gtcaactcaa acaatgcaga gacaacaaac
     1981 tgttgactta tcttttctat cgtaattcag cccttttggg aaatgagcat caacacacaa
     2041 tttagagtgg aagttgggtt aaggcagact cattttttac acattacaac cagtgattat
     2101 gtcaattatt atttaaaaaa aattcagtac agtatatgca catgctactt tgtttatgat
     2161 tgttagcttt tttaactaca gttcatttct atgctttctg tgcaaatgca caacagttac
     2221 tgatttactc tgatgataat tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga
     2281 cttacaatca agccccaaag tgttcatacg tctggcaaat tctgacttaa agttactttt
     2341 attcaaccag ctagtttttt tttgactgta aatgacacgt aagataataa gatgatgtac
     2401 atgaggcatc attgtagaaa aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt
     2461 gattttgttt gtgcgcactt acatacgtgt gcacaatatt tcaactctta agaaaaaaat
     2521 gcaattacca aaggcccatc agacaacaaa gttctctttc ctatcttttg ctcttcaaca
     2581 tttcagagat aactccatct taaactgtct tgtttttaga ccaacagtgc tagtgattgt
     2641 atgccggatg ctgagaacaa acaatgtttt atgcaggatg agtggaacta aagatgaaac
     2701 gttagttagc cgtaagccag tttcagttta gacactagta acccatcgtc tcaaagaggc
     2761 ttttgtcgaa tcggaccggt gacgtcaaat gaactttata aaggcataaa ttcatcgttt
     2821 gggtaaaaaa aacaatatga gaagagcaat cccctcccac ccccttaaat acattttgtg
     2881 tcttttcttt ggcattgaca tcttcagctg gccaagacga ctggtcagtt cttgcacatc
     2941 acctaaattc acaaagggaa tacttttttg aagctaacac atagttgaac gcaagtttgt
     3001 acaaaaaagt tggcaccatg actgagtaca aactggtggt ggttggagca ggtggtgttg
     3061 ggaaaagcgc actgacaatc cagctaatcc agaaccactt tgtagatgaa tatgatccca
     3121 ccatagagga ttcttacaga aaacaagtgg ttatagatgg tgaaacctgt ttgttggaca
     3181 tactggatac agctggacga gaagagtaca gtgccatgag agaccaatac atgaggacag
     3241 gcgaaggctt cctctgtgta tttgccatca ataatagcaa gtcatttgcg gatattaacc
     3301 tctacaggga acagattaag cgagtaaaag actcggatga tgtacctatg gtgctagtgg
     3361 gaaacaagtg tgatttgcca acaaggacag ttgatacaaa acaagcccac gaactggcca
     3421 agagttacgg gattccattc attgaaacct cagccaagac cagacagggt gttgaagatg
     3481 ctttttacac actggtaaga gaaatacgcc agtaccgaat gaaaaaactc aacagcagtg
     3541 atgatgggac tcagggttgt atgggattgc catgtgtggt gatgtaattg ccaactttct
     3601 tgtacaaagt gggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     3661 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     3721 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     3781 aggttcaggg ggaggtgtgg gaggtttttt ccaactttat tatacatagt tgataattca
     3841 ctggccgtcg ttttacggta ccatcgatga tgatccagac atgataagat acattgatga
     3901 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3961 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4021 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     4081 cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctcag attgtatcca
     4141 aaatatcatt ttttgtgttt taaagatgaa caaaggtctc agggaattgg aacgacatga
     4201 ggatgattaa ttaataaaaa aaaattcatt tttgggtgaa ctatccctaa aatcaaagca
     4261 atttgggcat tttttttcta attattatgg agaacttgca tcacagctct tccactagaa
     4321 aataaaacaa atttcagaaa tactgcagaa atacttttgg acttaatgtt aaataacaat
     4381 gtaaaaagtt tttccagggc atcgtggtgg tgcagtgggt agcattgtcg ccttacagca
     4441 agaaggtcgc tggttcgaga cttggttggg tcatttctgt gtggagtttg catgttctcc
     4501 ccatgttcgt gtgggtttcc tccgggtgct ccagtttcac ctacagccca aagacatgcg
     4561 ctataggtga attgggtaag ctaaattgtc taaagtgaat ggtactgtgg tgggtgtttc
     4621 ccagtgatgg gttgcagctg caagggcatc cgctggattt gtttgttggc ataagttggc
     4681 agttcattca actgtggcaa cccctgatta ataaaggggc taagccaaaa agaaaatgaa
     4741 tgactgaatg aaagtgaatc cagttgtaca tgcctagaca tgtcctccac ccttgcaaga
     4801 tgtacacccc aagaaatgga gctgcatttg aggacaacaa taagccaagc ctgcaaataa
     4861 aagacacctc aacagttttt attcaagcta ctctttatat catccgcgag aatccttcaa
     4921 aaatcaacta attgtttaca cgatttcatt cttgactaga ctctgacatg ataaatgaaa
     4981 aaatatatac acaagcacac taaatattct gaaatacaag attaatactt tacgaaattg
     5041 cgctcttcat gatctggatg taacactggt acttacaaga cacacacaca cacaaatcta
     5101 attgtctaac tttggttcat gaaatttagt tggcattgct aacagccatt gtcatgttcg
     5161 tccatactgc tgctgccgct actgttggaa ggtactgggg atccggagga caacagcggg
     5221 tcgctgctgc ccaccggaga cagggtgttg tccataagca tgtcctccag cttggtggag
     5281 gccttgtttg ggctgtcgta gacccccggc tcgccgtcct ccgtgggact gtcattgtag
     5341 ctgatggtgc cgttgttgag gtccagagtg gtaggacggg acatgtcagg actgggaagg
     5401 tgtgggtaca aattggatgt gcagtccccc atcccaggct cttgtttgat tgctcgagcc
     5461 actaactcag cggaataaag gctggaagaa gctacaacgg tgagtccatg agcacgggct
     5521 tgcatttcaa gttcctgaat acggagcagg agatgtctgt ttgcgtgttc tagtctcttc
     5581 tgtctgtttt ccagctcttt tgctttctgc tgctctttct gcaatttcct aatgtaatcc
     5641 actgatgctt tcaggatggt gcctttattc cacctcatgt ctggatcatt tgacttggga
     5701 attaaagtcc ccagctcctt aattctgtca ttgatattaa accgccttct tcgttcaatg
     5761 aggttgtggt tgtccttctt ttgtctctct tttgccaggg ctctgacttc tgcttctact
     5821 ggaaagccat caggtctttg ataagagtca aacttgccac atggtccagc tttgtccatt
     5881 atgtgcatca tgcccggaga tggagtaacg gataattccc ttttgacggc cggcaggctt
     5941 gaggggcagg agttactgat ggaaactcca gctggaggaa gagcatgatt gctgtacatg
     6001 tccagcaggt ttgctgaaac tggaatcgtg tttgtcattt gaagtcctgc atccatgaac
     6061 ccaagaatgt catcactgta gcttgattcc aaacttataa tgtcttcaat tacatcgtcc
     6121 atctctttct cacagttgag ggtgagaagg gccatagggc tgttgggagc gctggctccg
     6181 ggtcccgggg tcatgccgtg ctcggcgggc tggctggggc cgggccctgt gctggcctgt
     6241 gggctcagct tggctcccag tgcactggac aggtagtgct tcacctgctg cctctggctc
     6301 tgctggatgt ggtacttcga gggggtttcc aggtgggtct gaacctggta gtgactgtac
     6361 tcgagcatct ccaacatgtt caactatgtg ttagcttcaa aaaagtattc cctttgtgaa
     6421 tttaggtgat gtgcaagaac tgaccagtcg tcttggccag ctgaagatgt caatgccaaa
     6481 gaaaagacac aaaatgtatt taagggggtg ggaggggatt gctcttctca tattgttttt
     6541 tttacccaaa cgatgaattt atgcctttat aaagttcatt tgacgtcacc ggtccgattc
     6601 gacaaaagcc tctttgagac gatgggttac tagtgtctaa actgaaactg gcttacggct
     6661 aactaacgtt tcatctttag ttccactcat cctgcataaa acattgtttg ttctcagcat
     6721 ccggcataca atcactagca ctgttggtct aaaaacaaga cagtttaaga tggagttatc
     6781 tctgaaatgt tgaagagcaa aagataggaa agagaacttt gttgtctgat gggcctttgg
     6841 taattgcatt tttttcttaa gagttgaaat attgtgcaca cgtatgtaag tgcgcacaaa
     6901 caaaatcaca gacgtacagt tcatattcag attcatattc attttttttt ctacaatgat
     6961 gcctcatgta catcatctta ttatcttacg tgtcatttac agtcaaaaaa aaactagctg
     7021 gttgaataaa agtaacttta agtcagaatt tgccagacgt atgaacactt tggggcttga
     7081 ttgtaagtct tttttttttt tttgttgttg ttggatgtac tggcacaatt atcatcagag
     7141 taaatcagta actgttgtgc atttgcacag aaagcataga aatgaactgt agttaaaaaa
     7201 gctaacaatc ataaacaaag tagcatgtgc atatactgta ctgaattttt tttaaataat
     7261 aattgacata atcactggtt gtaatgtgta aaaaatgagt ctgccttaac ccaacttcca
     7321 ctctaaattg tgtgttgatg ctcatttccc aaaagggctg aattacgata gaaaagataa
     7381 gtcaacagtt tgttgtctct gcattgtttg agttgactac atcttgagca aatgtagcta
     7441 ttgtaaaatg gggatgagta tgtggtacag agaaccatct cgtcaatgga gtggaaactg
     7501 cattaacata gagagataga gagaacagct gtgtctaggc atggggtata agattctgat
     7561 ggtatgataa cctttgataa aaatatcaca gtttgatggt attgtgattg ccactctaaa
     7621 atatattatt ttttttaatg tcttagtaaa aaacaaaaac tttaaacata atatatttta
     7681 ttttgagaaa cattgaaaaa atattttgga acagaaaaca tgtcaagctt aataatttca
     7741 atgaatgatc gacttctgct gtcttcatta gtttcaaaaa cagatttctt tacaatttaa
     7801 aaagcatttt tgtatctctt ttctgctgaa aatgctgttg tcctaaaaaa caaaaaaata
     7861 aaaaataaat aaaaaatctt aaatatacct taggaacggt atagcagaaa atattggtgg
     7921 ttttaaaccc ttgacttttt caaaccgggg tataccttga aaacggttat cgtcctatgc
     7981 ctagctttgt caacaaccaa aaagcaactt tattaacatc ttaaggcaag tttaaaggca
     8041 acatctaaaa cagaatgaac ggttggagtt tagtgaagtt tatttataaa ctaatttcga
     8101 gagaatcacg tgcttatgac tgactacagc tggtcccaca ttagcctatg tatgattcac
     8161 caatcagaca attcctaact catcataggc cgccagagtt cctttcattt cctcgaaaga
     8221 gcggcacagc agcccagttg ttagcactgt tgcctcaaag caagaacgtc actggtccaa
     8281 gtctctaaca ggccagtcca tggttctgtg tggagtttac atgttgtcct cgtgctcatg
     8341 tggatatccc ctgggttctc cggttttctc ctacagtcca aaaacttgcg acataggtga
     8401 attgaacaaa accaaattag catagacaag ctcctaatca gcagaatatc tctccgtagc
     8461 aatctcttat tcgagatctg cgaagatacg gccacgggtg ctcttgatcc tgtggctgat
     8521 tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact
     8581 gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga
     8641 tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt
     8701 gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat
     8761 tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac
     8821 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
     8881 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
     8941 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc
     9001 ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc
     9061 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     9121 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9181 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9241 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9301 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9421 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9961 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    10021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10141 ctgacgctca gtggaacgaa aactcacgtt aaggcatttt ggtcatgaga ttatcaaaaa
    10201 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10261 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10321 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10381 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10441 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10501 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10561 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10621 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10681 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10741 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10801 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10861 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10921 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10981 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    11041 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    11101 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11161 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11221 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtg
//
