LOCUS       sequence-229439-        6826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  24x Widom 601 nucleosome sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Swygert SG, Senapati S, Bolukbasi MF, Wolfe SA, Lindsay S, Peterson 
            CL
  TITLE     SIR proteins create compact heterochromatin fibers.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Dec 4;115(49):12447-12452. doi: 
            10.1073/pnas.1810647115. Epub 2018 Nov 19.
  PUBMED    30455303
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1810647115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-12-4- 4"; volume: "115"; issue: "49"; pages: 
            "12447-12452"
COMMENT     Sequence Label: pBS_24x-601_MA1+MA3
FEATURES             Location/Qualifiers
     source          1..6826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     52..74
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     66..83
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     67..83
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4822..5613
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5706..6294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6195..6214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6448..6465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(6624..6651)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(join(6743..6826,1..3))
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
       61 gacgttgtaa aacgacggcc agtcttaagc gtctccccct gaacctgacc ccggaccaag
      121 tggtggctat tctagataac tgctcttagg cacaggatgt atatatctga cacgtgcctg
      181 gagactaggg agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt
      241 aagcggtgct agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt
      301 actggagaat tccagttcca tcagaagcac aggatgtata tatctgacac gtgcctggag
      361 actagggagt aatccccttg gcggttaaaa cgcgggggac agcgcgtacg tgcgtttaag
      421 cggtgctaga gctgtctacg accaattgag cggcctcggc accgggattc tccaggtact
      481 ggagaattcg gacgacagtg tgagcacagg atgtatatat ctgacacgtg cctggagact
      541 agggagtaat ccccttggcg gttaaaacgc gggggacagc gcgtacgtgc gtttaagcgg
      601 tgctagagct gtctacgacc aattgagcgg cctcggcacc gggattctcc aggtactgga
      661 gaattcccag ggatccttga gcacaggatg tatatatctg acacgtgcct ggagactagg
      721 gagtaatccc cttggcggtt aaaacgcggg ggacagcgcg tacgtgcgtt taagcggtgc
      781 tagagctgtc tacgaccaat tgagcggcct cggcaccggg attctccagg tactggagaa
      841 ttctgtttac accgacggca caggatgtat atatctgaca cgtgcctgga gactagggag
      901 taatcccctt ggcggttaaa acgcggggga cagcgcgtac gtgcgtttaa gcggtgctag
      961 agctgtctac gaccaattga gcggcctcgg caccgggatt ctccaggtac tggagaattc
     1021 atgctaacga gatcgcacag gatgtatata tctgacacgt gcctggagac tagggagtaa
     1081 tccccttggc ggttaaaacg cggggggtcg accgtacgtg cgtttaagcg gtgctagagc
     1141 tgtctacgac caattgagcg gcctcggcac cgggattctc caggtactgg agaattccgg
     1201 tccgtttacc agcacaggat gtatatatct gacacgtgcc tggagactag ggagtaatcc
     1261 ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt ttaagcggtg ctagagctgt
     1321 ctacgaccaa ttgagcggcc tcggcaccgg gattctccag gtactggaga attcgaaaaa
     1381 gagcgcaagc acaggatgta tatatctgac acgtgcctgg agactaggga gtaatcccct
     1441 tggcggttaa aacgcggggg acagcgcgta cgtgcgttta agcggtgcta gagctgtcta
     1501 cgaccaattg agcggcctcg gcaccgggat tctccaggta ctggagaatt ctacgcatat
     1561 actatgcaca ggatgtatat atctgacacg tgcctggaga ctagggagta atccccttgg
     1621 cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc ggtgctagag ctgtctacga
     1681 ccaattgagc ggcctcggca ccgggattct ccaggtactg gagaattcgc tcaagcttga
     1741 tcgcacagga tgtatatatc tgacacgtgc ctggagacta gggagtaatc cccttggcgg
     1801 ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt gctagagctg tctacgacca
     1861 attgagcggc ctcggcaccg ggattctcca ggtactggag aattcagccc acgcagacgg
     1921 cacaggatgt atatatctga cacgtgcctg gagactaggg agtaatcccc ttggcggtta
     1981 aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     2041 gagcggcctc ggcaccggga ttctccaggt actggagaat tcgcgtgtaa tacactgcac
     2101 aggatgtata tatctgacac gtgcctggag actagggagt aatccccttg gcggttaaaa
     2161 cgcgggggac agcgcgtacg tgcgtttaag cggtgctaga gctgtctacg accaattgag
     2221 cggcctcggc accgggattc tccaggtaga attcactaga taactgctct taggcacagg
     2281 atgtatatat ctgacacgtg cctggagact agggagtaat ccccttggcg gttaaaacgc
     2341 gggggacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc aattgagcgg
     2401 cctcggcacc gggattctcc aggtactgga gaattccagt tccatcagaa gcacaggatg
     2461 tatatatctg acacgtgcct ggagactagg gagtaatccc cttggcggtt aaaacgcggg
     2521 ggacagcgcg tacgtgcgtt taagcggtgc tagagctgtc tacgaccaat tgagcggcct
     2581 cggcaccggg attctccagg tactggagaa ttcggacgac agtgtgagca caggatgtat
     2641 atatctgaca cgtgcctgga gactagggag taatcccctt ggcggttaaa acgcggggga
     2701 cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac gaccaattga gcggcctcgg
     2761 caccgggatt ctccaggtac tggagaattc ccagggatcc ttgagcacag gatgtatata
     2821 tctgacacgt gcctggagac tagggagtaa tccccttggc ggttaaaacg cgggggacag
     2881 cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac caattgagcg gcctcggcac
     2941 cgggattctc caggtactgg agaattctgt ttacaccgac ggcacaggat gtatatatct
     3001 gacacgtgcc tggagactag ggagtaatcc ccttggcggt taaaacgcgg gggacagcgc
     3061 gtacgtgcgt ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg
     3121 gattctccag gtactggaga attcatgcta acgagatcgc acaggatgta tatatctgac
     3181 acgtgcctgg agactaggga gtaatcccct tggcggttaa aacgcggggg gtcgaccgta
     3241 cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat
     3301 tctccaggta ctggagaatt ccggtccgtt taccagcaca ggatgtatat atctgacacg
     3361 tgcctggaga ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgtacgt
     3421 gcgtttaagc ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattct
     3481 ccaggttgcg tgggcgggaa agaattcgct agcacaggat gtatatatct gacacgtgcc
     3541 tggagactag ggagtaatcc ccttggcggt taaaacgcgg gggacagcgc gtacgtgcgt
     3601 ttaagcggtg ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg gattctccag
     3661 gtactggaga attctacgca tatactatgc acaggatgta tatatctgac acgtgcctgg
     3721 agactaggga gtaatcccct tggcggttaa aacgcggggg acagcgcgta cgtgcgttta
     3781 agcggtgcta gagctgtcta cgaccaattg agcggcctcg gcaccgggat tctccaggta
     3841 ctggagaatt cgctcaagct tgatcgcaca ggatgtatat atctgacacg tgcctggaga
     3901 ctagggagta atccccttgg cggttaaaac gcgggggaca gcgcgtacgt gcgtttaagc
     3961 ggtgctagag ctgtctacga ccaattgagc ggcctcggca ccgggattct ccaggtactg
     4021 gagaattcag cccacgcaga cggcacagga tgtatatatc tgacacgtgc ctggagacta
     4081 gggagtaatc cccttggcgg ttaaaacgcg ggggacagcg cgtacgtgcg tttaagcggt
     4141 gctagagctg tctacgacca attgagcggc ctcggcaccg ggattctcca ggtactggag
     4201 aattcgcgtg taatacactg cacaggatgt atatatctga cacgtgcctg gagactaggg
     4261 agtaatcccc ttggcggtta aaacgcgggg gacagcgcgt acgtgcgttt aagcggtgct
     4321 agagctgtct acgaccaatt gagcggcctc ggcaccggga ttctccaggt agaattcact
     4381 agtgcggcat gcggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
     4441 tgccaggacc atggccgaga cgtctagacc agccaggaca gaaatgcctc gacttcgctg
     4501 ctacccaagg ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg
     4561 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg
     4621 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt
     4681 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac
     4741 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc
     4801 cctaaaacaa agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     4861 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     4921 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     4981 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     5041 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     5101 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     5161 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     5221 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     5281 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     5341 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     5401 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     5461 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     5521 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     5581 aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc
     5641 cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5701 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5761 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5821 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     5881 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5941 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6001 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6061 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
     6121 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6181 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6241 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6301 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6361 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6421 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6481 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6541 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
     6601 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
     6661 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
     6721 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
     6781 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttat
//
