LOCUS       sequence-229491-       11215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Chloroplast-targeted expression of CURT_fluoE controlled by the CaMV
            35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11215)
  AUTHORS   Behrendorff JBYH, Sandoval-Ibanez OA, Sharma A, Pribil M
  TITLE     Membrane-bound protein scaffolding in diverse hosts using thylakoid 
            protein CURT1A.
  JOURNAL   ACS Synth Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418.
  PUBMED    30884945
REFERENCE   2  (bases 1 to 11215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2019 Mar 18. doi: 10.1021/acssynbio.8b00418."
COMMENT     Sequence Label: pN_35S/CTP-fluoE
FEATURES             Location/Qualifiers
     source          1..11215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(264..266,267..977)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(306..327)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(567..586)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     914..935
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1314..2024
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1727..1746
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2057..2081)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2401..2425
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3756..4385
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4814..5887
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5953..6147
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6383..6405)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6491..6631
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6546..6565
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6646..6663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6817..7405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6897..6916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7651..8442)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    8963..8987
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(9080..9332)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9355..9927)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(9969..10148)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        10870..11214
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11158..11178
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt acaatttact
       61 attctagtcg acctgcaggc ggccgcacta gtgatatcat ggcttcctct atgctctctt
      121 ccgctactat ggttgcctct ccggctcagg ccactatggt cgctcctttc aacggactta
      181 agtcctccgc tgccttccca gccacccgca aggctaacaa cgacattact tccatcacaa
      241 gcaacggcgg aagagttaac tgcgtgagca agggcgagga gctgttcacc ggggtggtgc
      301 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      361 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      421 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc
      481 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      541 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      601 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      661 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      721 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
      781 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      841 tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa gaccccaacg
      901 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      961 tggacgagct gtacaaggga ggtggtggat ctgcttcttc agaagagacc tcgtccattg
     1021 ataccaacga actcatcaca gatttgaagg aaaagtggga tggtcttgag aacaaatcta
     1081 ctgtacttat atatggagga ggagccattg ttgctgtttg gttatcttcc attgttgttg
     1141 gtgccatcaa ctctgttcct ctgcttccga aagttatgga acttgtcgga ctagggtaca
     1201 ctggatggtt tgtctacaga taccttctct tcaagtcaag cagaaaggaa ttggctgagg
     1261 atattgaatc cttgaagaag aagatcgcag gaagcgaagg aggtggaggt tctatggtga
     1321 gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     1381 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     1441 acgaggcctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     1501 gggacatcct gtcccctcag ttcatgtacg gctccaaggt ctacattaag cacccagccg
     1561 acatccccga ctacttcaag ctgtccttcc ccgagggctt caggtgggag cgcgtgatga
     1621 acttcgagga cggcggcatt attcacgtta accaggactc ctccctgcag gacggcgtgt
     1681 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     1741 agaagaccat gggctgggag gcctccgagg agcggatgta ccccgaggac ggcgccctga
     1801 agagcgagat caagaagagg ctgaagctga aggacggcgg ccactacgcc gccgaggtca
     1861 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcgacatca
     1921 agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1981 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaaagctt ggctgttttg
     2041 gcggatgaga gaagtcgttc agctttcttg tacaaagtgg tgatatcccg cggccatgct
     2101 agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag
     2161 aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg
     2221 tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata
     2281 aaatttctaa ttcctaaaac caaaatccag tgacctgcag gcatgcgacg tcgggcccaa
     2341 gcttagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt
     2401 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
     2461 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
     2521 gggttcccct cgggatcaaa gtactttaaa gtactttaaa gtactttaaa gtactttgat
     2581 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
     2641 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
     2701 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
     2761 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
     2821 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
     2881 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
     2941 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
     3001 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
     3061 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     3121 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     3181 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     3241 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     3301 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     3361 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     3421 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     3481 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     3541 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     3601 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     3661 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     3721 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     3781 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     3841 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     3901 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     3961 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     4021 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     4081 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     4141 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     4201 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     4261 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     4321 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     4381 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     4441 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     4501 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     4561 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     4621 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     4681 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     4741 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     4801 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
     4861 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
     4921 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
     4981 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
     5041 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
     5101 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
     5161 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
     5221 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
     5281 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
     5341 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
     5401 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
     5461 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
     5521 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
     5581 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
     5641 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
     5701 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
     5761 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
     5821 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
     5881 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
     5941 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
     6001 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
     6061 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
     6121 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
     6181 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
     6241 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
     6301 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
     6361 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6421 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     6481 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     6541 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     6601 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     6661 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     7261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7441 tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat ctcccaattt
     7501 gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg
     7561 ctgtgagcaa ttatgtgctt agtgcatcta acgcttgagt taagccgcgc cgcgaagcgg
     7621 cgtcggcttg aacgaatttc tagctagaca ttatttgccg actaccttgg tgatctcgcc
     7681 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
     7741 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
     7801 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
     7861 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
     7921 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
     7981 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
     8041 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
     8101 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
     8161 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
     8221 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
     8281 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
     8341 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
     8401 tgatagttga gtcgatactt cggcgatcac cgcttccccc atgatgttta actttgtttt
     8461 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     8521 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
     8581 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     8641 ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta
     8701 tgtccactgg gttcgtgccc gaattgatca caggcagcaa cgctctgtca tcgttacaat
     8761 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     8821 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     8881 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     8941 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     9001 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattgaatt
     9061 atcagcttgc atgccggtcg atctagtaac atagatgaca ccgcgcgcga taatttatcc
     9121 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
     9181 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
     9241 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
     9301 aagaaacttt attgccaaat gtttgaacga tctgcttgac tctaggggtc atcattaggg
     9361 gcagggcatg ctcatgtaga gcgcctgctc gccgtccgag gcggtgccgt cgtacagggc
     9421 ggtgtccagg ccgcagaggg tgaaccccat ccgccggtac gcgtggatcg ccggtgcgtt
     9481 gacgttggtg acctccagcc agaggtgccc ggcaccccgc tcgcgggcga actccgtcgc
     9541 gagccccatc aacgcgcgcc cgaccccgtg cccccggtgc tccggggcga cctcgatgtc
     9601 ctcgacggtc agccggcggt tccagccgga gtacgagacg accacgaagc ccgccaggtc
     9661 gccgtcgtcc ccgtacgcga cgaacgtccg agagtccggg tcgccgtcct ccccgtcgtc
     9721 cgattcgtcg tccgattcgt cgtcggggaa caccttggtc aggggcgggt ccaccggcac
     9781 ctcccgcagg gtgaagccgt ccccggtggc ggtgacgcgg aagacggtgt cggtggtgaa
     9841 ggacccatcc agtgcctcga tggcctcggc gtcccccggg acactggtgc ggtaccggta
     9901 agccgtgtcg tcaagagtgg tacccatggt agatcccctc gatcgagttg agagtgaata
     9961 tgagactcta attggatacc gaggggaatt tatggaacgt cagtggagca tttttgacaa
    10021 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
    10081 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctcagtggct ccttcaacgt
    10141 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
    10201 acgtgactcc cttaattctc cgctcatgat cgataattcg agctctccca tatggtcgac
    10261 tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc tctatctcta
    10321 cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca ccgataatga
    10381 gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta gagaggccta
    10441 cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga tcaaatacct
    10501 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
    10561 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
    10621 tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc ctactgaatc
    10681 aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg tgaagactgg
    10741 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
    10801 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
    10861 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
    10921 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
    10981 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca gtggtcccaa
    11041 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
    11101 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
    11161 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggact
//
