LOCUS       sequence-229673-        9927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on nuclear body formation and 
            splicing of full-length TDP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1410 GFP-TDP43 F-S
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    144..260
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        283..586
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        587..790
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     741..761
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     787..811
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2869..2893)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2929..3517
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2982..3002)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3400..3411)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3531..4030
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3566..3589)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3963..3982
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4051..4650
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4051..4070)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4547..4567
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4788..5021
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5093..5214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5130..5149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5184..5203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5254..5389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5409..5428)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5410..5428)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5438..5455)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5438..5454)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5447..5469)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5596..6051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5683..5702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5893..5914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6077..6181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6182..6250,6251..7042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6400..6419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7213..7801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7702..7721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7955..7972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8089..8110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8125..8130,8131..8148,8149..8155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8163..8179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8168..8190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8187..8203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8187..8203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8222..8242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8224..8242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8270..8496
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8497..8677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8724..8849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9342..9575
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9760..9804
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
       61 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
      121 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
      181 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      241 attacaaaat tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt
      301 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      361 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      421 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      481 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      541 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      601 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      661 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      721 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      781 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      841 cctccataga agacaccgac tctagtccag tgtggtggaa ttctgcagat taccaccggt
      901 gccaccatgg gaagtaaagg agaggaacta tttacaggta ctgtgcctat caaagtcgag
      961 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgtg gcgagggcga gggcgatgcc
     1021 accgagggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc ggtgccgtgg
     1081 ccgaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta cccggaccac
     1141 atgaagcgtc acgacttctt caagtccgcc atgccggaag gctacgtcca ggagcgcacc
     1201 atcgagttca aggacgacgg cacctacaag acccgcgcgg aggtgaagtt cgagggcgac
     1261 accctggtga accgcatcga gctgaagggc aacgacttca aggaggacgg caacatcctg
     1321 ggtcacaagc tggagtacaa ccacaacagc cacaacgtcc gtatcgaagc cgacaagcag
     1381 aagaacggca tcaaggctaa cttcaagatc cgccacaacg ttgaggacgg cagccagcag
     1441 gaagccgacc acaaacagca gaacaccccg atcggcgacg gcccggtgcg cctgccggac
     1501 aaccactatc tgagcaccca gaccacgctg agcaaagacc cgaacgagaa gcgcgatcac
     1561 atggtcctga aggagttcgt gacggcagcc ggtatcacta aaggtgaaga tgaaagtgga
     1621 tccatgtctg aatatattcg ggtaaccgaa gatgagaacg atgagcccat tgaaatacca
     1681 tcggaagacg atgggacggt gctgctctcc acggttacag cccagtttcc aggggcgtgt
     1741 gggcttcgct acaggaatcc agtgtctcag tgtatgagag gtgtccggct ggtagaagga
     1801 attctgcatg ccccagatgc tggctgggga aatctggtgt atgttgtcaa ctatccaaaa
     1861 gataacaaaa gaaaaatgga tgagacagat gcttcatcag cagtgaaagt gaaaagagca
     1921 gtccagaaaa catccgattt aatagtgttg ggtctcccat ggaaaacaac cgaacaggac
     1981 ctgaaagagt attttagtac ctttggagaa gttcttatgg tgcaggtcaa gaaagatctt
     2041 aagactggtc attcaaaggg gtttggcttt gttcgtttta cggaatatga aacacaagtg
     2101 aaagtaatgt cacagcgaca tatgatagat ggacgatggt gtgactgcaa acttcctaat
     2161 tctaagcaaa gccaagatga gcctttgaga agcagaaaag tgtttgtggg gcgctgtaca
     2221 gaggacatga ctgaggatga gctgcgggag ttcttctctc agtacgggga tgtgatggat
     2281 gtcttcatcc ccaagccatt cagggccttt gcctttgtta catttgcaga tgatcagatt
     2341 gcgcagtctc tttgtggaga ggacttgatc attaaaggaa tcagcgttca tatatccaat
     2401 gccgaaccta aacataattc aaaccggcag ttggaaagat cggggcgcag tggtggcaac
     2461 ccaggcggtt ctggtaatca gggcggctcg ggaaattcca gaggcggggg tgcagggctt
     2521 ggcaacaatc aggggtccaa tatgggcggt ggtatgaact caggtgccag ctcgattaat
     2581 ccggccatga tggcggcagc gcaggcggcg ctgcagtctt cctggggtat gatgggtatg
     2641 ttagcatccc aacagaacca gagtggcccg tcggggaata accaaaatca ggggaatatg
     2701 cagcgcgaac cgaatcaagc ttcaggtagt ggtaacaaca gttatagtgg atcgaacagt
     2761 ggcgccgcaa ttggatgggg ttccgcatcc aatgcaggct caggcagcgg ctcgaatggc
     2821 ggctcaggtt ctagtatgga ttcgaagagc tctggttggg gaatgtagac ccagctttct
     2881 tgtacaaagt ggttgatcct ctatccagca cagtggcggc cgctcgacaa tcaacctctg
     2941 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3001 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3061 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3121 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3181 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3241 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3301 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3361 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3421 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3481 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga
     3541 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     3601 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     3661 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     3721 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     3781 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     3841 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     3901 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     3961 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     4021 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     4081 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     4141 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     4201 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     4261 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     4321 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     4381 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     4441 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     4501 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     4561 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     4621 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     4681 gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta
     4741 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     4801 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     4861 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     4921 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     4981 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     5041 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     5101 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5161 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5221 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5281 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5341 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     5401 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     5461 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     5521 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     5581 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5641 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5701 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5761 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5821 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5881 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5941 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6001 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6061 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     6181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     6241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     6301 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     6361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     6421 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     6481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     6541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     6601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     6661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     6721 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     7561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8041 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8101 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     8161 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     8221 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     8281 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     8341 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     8401 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     8461 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     8521 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     8581 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     8641 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     8701 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     8761 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     8821 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     8881 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     8941 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     9001 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     9061 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     9121 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     9181 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     9241 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     9301 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9361 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9421 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     9481 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     9541 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     9601 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     9661 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     9721 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     9781 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     9841 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     9901 ctttctatag tgaatagagt taggcag
//
