LOCUS       sequence-229677-        9927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test the effect of sequence on nuclear body formation and 
            splicing of full-length TDP43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Schmidt HB, Barreau A, Rohatgi R
  TITLE     Phase separation-deficient TDP43 remains functional in splicing.
  JOURNAL   Nat Commun. 2019 Oct 25;10(1):4890. doi: 10.1038/s41467-019-12740-2.
  PUBMED    31653829
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-25"; volume: "10(1):4890. doi"; pages: " 
            10.1038/s41467-019-12740-2"
COMMENT     Sequence Label: pHBS1411 GFP-TDP43 FYW-S
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    82..198
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..699
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     725..749
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      839..848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2807..2831)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2867..3455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2920..2940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3338..3349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3469..3968
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3504..3527)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3901..3920
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3989..4588
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3989..4008)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4485..4505
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4726..4959
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5031..5152
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5068..5087)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5122..5141
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5192..5327
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5347..5366)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5348..5366)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5376..5393)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5376..5392)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5385..5407)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5534..5989
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5621..5640)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5831..5852
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6015..6119
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6120..6188,6189..6980)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6338..6357)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7151..7739
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7640..7659
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7893..7910
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8027..8048
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8063..8068,8069..8086,8087..8093)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8101..8117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8106..8128
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8125..8141
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8125..8141
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8160..8180
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8162..8180
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8208..8434
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8435..8615
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8662..8787
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9280..9513
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9698..9742
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
       61 atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg
      121 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
      181 tacaaaattc aaaattttat cgataagctt gggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      781 tccatagaag acaccgactc tagtccagtg tggtggaatt ctgcagatta ccaccggtgc
      841 caccatggga agtaaaggag aggaactatt tacaggtact gtgcctatca aagtcgagct
      901 ggacggcgac gtaaacggcc acaagttcag cgtgcgtggc gagggcgagg gcgatgccac
      961 cgagggcaag ctgaccctga agttcatctg caccaccggc aagctgccgg tgccgtggcc
     1021 gaccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc cggaccacat
     1081 gaagcgtcac gacttcttca agtccgccat gccggaaggc tacgtccagg agcgcaccat
     1141 cgagttcaag gacgacggca cctacaagac ccgcgcggag gtgaagttcg agggcgacac
     1201 cctggtgaac cgcatcgagc tgaagggcaa cgacttcaag gaggacggca acatcctggg
     1261 tcacaagctg gagtacaacc acaacagcca caacgtccgt atcgaagccg acaagcagaa
     1321 gaacggcatc aaggctaact tcaagatccg ccacaacgtt gaggacggca gccagcagga
     1381 agccgaccac aaacagcaga acaccccgat cggcgacggc ccggtgcgcc tgccggacaa
     1441 ccactatctg agcacccaga ccacgctgag caaagacccg aacgagaagc gcgatcacat
     1501 ggtcctgaag gagttcgtga cggcagccgg tatcactaaa ggtgaagatg aaagtggatc
     1561 catgtctgaa tatattcggg taaccgaaga tgagaacgat gagcccattg aaataccatc
     1621 ggaagacgat gggacggtgc tgctctccac ggttacagcc cagtttccag gggcgtgtgg
     1681 gcttcgctac aggaatccag tgtctcagtg tatgagaggt gtccggctgg tagaaggaat
     1741 tctgcatgcc ccagatgctg gctggggaaa tctggtgtat gttgtcaact atccaaaaga
     1801 taacaaaaga aaaatggatg agacagatgc ttcatcagca gtgaaagtga aaagagcagt
     1861 ccagaaaaca tccgatttaa tagtgttggg tctcccatgg aaaacaaccg aacaggacct
     1921 gaaagagtat tttagtacct ttggagaagt tcttatggtg caggtcaaga aagatcttaa
     1981 gactggtcat tcaaaggggt ttggctttgt tcgttttacg gaatatgaaa cacaagtgaa
     2041 agtaatgtca cagcgacata tgatagatgg acgatggtgt gactgcaaac ttcctaattc
     2101 taagcaaagc caagatgagc ctttgagaag cagaaaagtg tttgtggggc gctgtacaga
     2161 ggacatgact gaggatgagc tgcgggagtt cttctctcag tacggggatg tgatggatgt
     2221 cttcatcccc aagccattca gggcctttgc ctttgttaca tttgcagatg atcagattgc
     2281 gcagtctctt tgtggagagg acttgatcat taaaggaatc agcgttcata tatccaatgc
     2341 cgaacctaag cataatagca acagacaact cgaacgaagc gggcggagtg ggggcaaccc
     2401 cggcggatct ggtaatcaag gcggaagcgg caatagccgg ggtgggggag ccggtctcgg
     2461 caataatcaa ggatctaaca tgggcggcgg tatgaactct ggcgcatcca gtatcaaccc
     2521 ggccatgatg gcagcagcac aggccgccct gcaaagttca tggggaatga tgggtatgtt
     2581 ggcaagtcag cagaaccagt caggaccttc cgggaataat caaaaccaag ggaacatgca
     2641 acgcgaaccc aatcaagcct caggatcagg caataatagc agttccggct caaattccgg
     2701 cgcggcgata gggtctggct ccgcctcaaa tgcgggaagc ggatcaggga gtaacggagg
     2761 atcaggctca tctatggatt caaaatccag cggcagtggt atgtagaccc agctttcttg
     2821 tacaaagtgg ttgatcctct atccagcaca gtggcggccg ctcgacaatc aacctctgga
     2881 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2941 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3001 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     3061 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     3121 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     3181 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     3241 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     3301 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3361 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3421 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctaccgg gtaggggagg
     3481 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     3541 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     3601 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     3661 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     3721 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     3781 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     3841 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     3901 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     3961 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     4021 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     4081 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     4141 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     4201 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4261 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4321 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     4381 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     4441 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     4501 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     4561 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     4621 aaggagcgca cgaccccatg catctcgagg gcccggtacc tttaagacca atgacttaca
     4681 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc
     4741 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     4801 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     4861 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     4921 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     4981 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5161 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5221 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5281 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     5341 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     5401 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5461 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5521 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5581 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5641 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5701 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     5761 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5821 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5881 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5941 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     6001 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6061 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7261 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7681 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7801 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7861 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7921 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7981 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8041 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8101 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     8161 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     8221 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     8281 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     8341 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     8401 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     8461 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8521 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8581 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     8641 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     8701 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     8761 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     8821 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     8881 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     8941 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     9001 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     9061 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     9121 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9181 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9241 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9301 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9361 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9421 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9481 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9541 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     9601 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     9661 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     9721 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9781 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9841 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9901 aaccccgagg ggacccgaca ggcccga
//
