LOCUS       sequence-229825-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gli protein-dependent EGFP reporter for analysis of Hedgehog 
            signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Hyman JM, Firestone AJ, Heine VM, Zhao Y, Ocasio CA, Han K, Sun M, 
            Rack PG, Sinha S, Wu JJ, Solow-Cordero DE, Jiang J, Rowitch DH, Chen
            JK
  TITLE     Small-molecule inhibitors reveal multiple strategies for Hedgehog 
            pathway blockade.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):14132-7. doi: 
            10.1073/pnas.0907134106. Epub 2009 Aug 5.
  PUBMED    19666565
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0907134106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-08-18- 18"; volume: "106"; issue: "33"; pages: "14132-7"
COMMENT     Sequence Label: pGL3b-8XGliBS:EGFP
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    78..126
                     /note="synthetic polyadenylation signal"
     misc_feature    140..231
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     180..199
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    246..380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(283..302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     337..356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      896..905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag
       61 gtacttggag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
      121 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
      181 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgatagg
      241 taccgaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      301 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      361 tatcttatca tgtctggatc cccgggaaca gattcgcgat cgacctcgac aagcagggaa
      421 cacccaagta gaagttccga caagcaggga acacccaagt agaagctctc gacctcgaca
      481 agcagggaac acccaagtag aagctccgac aagcagggaa cacccaagta gaagctctcg
      541 acctcgacaa gcagggaaca cccaagtaga agctccgaca agcagggaac acccaagtag
      601 aagctctcga cctcgacaag cagggaacac ccaagtagaa gctccgacaa gcagggaaca
      661 cccaagtaga agctctcgat cgcgaatctg ttcccgggga tcctctagag tcgacctgca
      721 gggccagccc aggctccggg gcacgtaaaa gcggggctgt gagaccggag agcacggagc
      781 gaccagccag ggctgagctg cggagacggt gagcagggct gtgcgggcag ccaagctagc
      841 ccgggctcga gatctgcgat ctaagtaagc ttggcattcc ggtactgttg gtaaagccac
      901 catggtgagc aagggc
//
