LOCUS       sequence-22995-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Kohli M, Rago C, Lengauer C, Kinzler KW, Vogelstein B
  TITLE     Facile methods for generating human somatic cell gene knockouts 
            using recombinant adeno-associated viruses.
  JOURNAL   Nucleic Acids Res. 2004  . 32(1):e3.
  PUBMED    14704360
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2004  . 32(1):e3."
COMMENT     Sequence Label: pAAV-Neo-PTEN
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..549)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(98..117)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     491..514
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    561..594
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tgcgtgcaat ccatcttgtt caatggccga tcccatattg gctgcaggtc gaaaggcccg
       61 gagatgagga agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg
      121 ggcctccggg ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc
      181 cggacccacc ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc
      241 cgctgcccca aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac
      301 tagtgagacg tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg
      361 ggaacttcct gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag
      421 ccggttggcg cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc
      481 gccaagtgcc cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct
      541 cccctacccg gtagggcgcg ataacttcgt ataatgtatg ctatacgaag ttatccgcgg
      601 tggagctcca gcttttgttc cctt
//
