LOCUS       sequence-229964-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa 
            N-truncation, 9aa C-truncation, F64L, and C203T (numbered with 
            original sequence); see https://www.fpbase.org/protein/degfp3/).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C1m
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..53)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(293..312)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     640..661
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aatggagctt ttcactggcg ttgttcccat cctggtcgag ctggacggcg acgtaaacgg
       61 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      121 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      181 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      241 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      301 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      361 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      421 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      481 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      541 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      601 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      661 gaccgccgcc gggatctaaa ggt
//
