LOCUS       sequence-229974-         719 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Cerulean (modified CFP; 
            variant of https://www.fpbase.org/protein/cerulean/) - Please see 
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 719)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C13m
FEATURES             Location/Qualifiers
     source          1..719
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..715
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             2..715
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(32..60)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     655..675
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
       61 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      121 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      181 tgtcactact ttgacttggg gtgttcaatg ctttgctaga tacccagatc atatgaaaca
      241 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      301 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      361 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      421 attggaatac aacgctattt cagataatgt atacatcact gcagacaaac aaaagaatgg
      481 aatcaaagct aatttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      541 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      601 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagatc acatggtcct
      661 tcttgagttt gtaacagctg ctgggattac actaggcatg gatgaactat acaaaaggt
//
