LOCUS       sequence-230108-         117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, 
            prefixed by self-cleaving RiboJ for upstream context independence). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 117)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U25m
FEATURES             Location/Qualifiers
     source          1..117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        7..56
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             86..108
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tactagctgt caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc
       61 tacaaataat tttgtttaaa ataattttgt ttaactttaa gaaggagata tacaatg
//
