LOCUS       sequence-230138-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MoClo golden gate assembly AE destination vector DVCK-AE-GFP 
            (ColE1/kan resistance destination vector with constitutive GFP 
            expression for screening..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V35m
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        49..83
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     CDS             114..827
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(144..172)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     767..787
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      846..917
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      933..960
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ggagagagac ctgcaccata tgcggtgaga ctagtggaag acatggagtt gacagctagc
       61 tcagtcctag gtactgtgct agctactaga gtcacacagg aaagtactaa atgatgcgta
      121 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
      181 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
      241 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
      301 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
      361 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
      421 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
      481 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
      541 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
      601 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
      661 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
      721 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga tcacatggtc cttcttgagt
      781 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taaaggtcca
      841 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      901 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      961 tttatagctt atgtcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     1021 gctgcattaa tgaatcggcc aacgcgcggg ggtctctgct t
//
