LOCUS       sequence-230172-       13405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for 3xFLAG-tagged MGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13405)
  AUTHORS   Stielow B, Finkernagel F, Stiewe T, Nist A, Suske G
  TITLE     MGA, L3MBTL2 and E2F6 determine genomic binding of the non-canonical
            Polycomb repressive complex PRC1.6.
  JOURNAL   PLoS Genet. 2018 Jan 30;14(1):e1007193. doi: 
            10.1371/journal.pgen.1007193.
  PUBMED    29381691
REFERENCE   2  (bases 1 to 13405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2018-01-30"; volume: "14(1):e1007193. doi"; pages: "
            10.1371/journal.pgen.1007193"
COMMENT     Sequence Label: pN3-MGA
FEATURES             Location/Qualifiers
     source          1..13405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             155..949
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             155..949
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(209..228)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     819..838
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1137..1156)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1181..1228
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1557..2145
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2046..2065
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2320..2623
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2624..2827
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2778..2798
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2882..2947
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     RBS             8201..8209
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    12452..12573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12489..12508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12543..12562
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(12580..13035)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12717..12738)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12929..12948
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        13062..13166
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(13168..13405,1..120)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        13168..13359
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(13191..13211)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(13376..13405,1..106)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
       61 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
      121 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
      181 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
      241 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
      301 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg
      361 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
      421 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
      481 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
      541 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
      601 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
      661 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg
      721 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
      781 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
      841 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
      901 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
      961 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     1021 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     1081 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa
     1141 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat
     1201 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac
     1261 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc
     1321 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca
     1381 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt
     1441 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1501 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1561 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1621 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1681 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1741 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1801 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1861 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1921 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1981 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2041 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2101 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2161 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2221 tcccctgatt ctgtggataa ccgtattacc gccatgcatt agttattaat agtaatcaat
     2281 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     2341 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2401 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2461 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2521 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2581 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2641 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2701 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2761 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2821 cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggactcaga tctccaccat
     2881 ggactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga
     2941 tgacaagaag cttcgaattc tgcagtcgag ggaggagaaa cagcagatta tattggctaa
     3001 tcaagatggt ggaacagtgg caggagcagc acctaccttc tttgtcatct taaagcagcc
     3061 aggaaatggc aaaactgatc aaggaatttt ggttactaat caggatgcct gtgctttggc
     3121 tagtagtgtg tcatcaccag taaaatctaa agggaagatt tgccttccag ctgattgtac
     3181 tgtgggtgga atcactgtta ccctcgataa caatagtatg tggaatgagt tctatcatcg
     3241 aagcacagag atgattctga ccaagcaagg aagacgcatg tttccttact gtcgttattg
     3301 gataacaggt ttagattcaa atttgaagta tattcttgtc atggatatat ctcctgtgga
     3361 taaccatcgt tataagtgga atggtcgttg gtgggaacct agtgggaagg ctgaacctca
     3421 tgttttgggg agggttttca ttcatccaga atctccttcc acaggtcatt attggatgca
     3481 tcaaccagta tctttctata aactcaaact taccaacaat acactggacc aagaagggca
     3541 tatcatcttg cactctatgc atcgttacct gccgaggctt catttggtgc ctgcagaaaa
     3601 ggctgtggag gtgatacaat taaatggccc tggtgtccac acttttacct tcccacagac
     3661 tgaattcttt gcagtaacag cttatcagaa cattcagatt actcagctga aaatagatta
     3721 caatccattt gccaaaggct ttcgggatga tgggctgaat aataagcccc agagagatgg
     3781 aaaacaaaag aacagctctg accaagaagg gaataatatt tccagttctt ctggtcatcg
     3841 ggtccgtctt acagaaggtc aggggtcaga gatacaacca ggtgatttgg atcctttgtc
     3901 aaggggtcat gaaacatcag gcaagggttt ggagaagact tcccttaata taaaacgaga
     3961 ctttcttggt ttcatggata ctgattcagc acttagtgaa gttcctcaat tgaagcaaga
     4021 gatttctgaa tgtcttattg ccagcagttt tgaagatgac tcccgtgtag cctcaccgtt
     4081 agaccagaac ggaagcttca atgttgttat taaagaggaa cctctagatg attatgacta
     4141 cgaacttggt gagtgcccag aaggggtcac tgtgaaacag gaagagacag atgaagagac
     4201 ggatgtatac tcaaacagtg atgatgatcc tatactagag aaacagctaa agaggcacaa
     4261 taaagttgac aacccagaag ctgaccatct atcttctaaa tggcttccaa gcagcccatc
     4321 aggtgttgct aaagctaaaa tgttcaaatt agacactgga aagatgccag tagtctatct
     4381 ggagccctgt gctgtcacca gaagcacagt taagatttct gaactccccg ataacatgct
     4441 ttccacatct cgaaaggata aatcttctat gttggcagaa ttggaatatt tgcctacata
     4501 cattgaaaat tccaatgaga ctgccttctg cttaggcaag gaatcagaaa atggtcttag
     4561 aaaacattca ccagatctca gagtggtaca aaaatatccc ttactgaaag agcctcagtg
     4621 gaaatatcct gatatatctg acagcattag cacagaaaga atactcgacg attcaaagga
     4681 ttcagttgga gactcacttt caggaaaaga ggacttgggc agaaagagaa caactatgct
     4741 taagattgca acagccgcaa aggtagtgaa tgctaatcag aatgcctctc caaatgtccc
     4801 tggaaaaaga ggaaggccac gaaaattgaa actctgtaag gcaggacgac cacctaagaa
     4861 cacaggaaag tctttaattt ctacaaagaa tacacctgta agccctggga gtacctttcc
     4921 agatgtgaag cctgatctgg aagatgtgga tggtgttctc tttgtttcct ttgaatcaaa
     4981 ggaagctcta gacattcatg cagttgatgg gacaacagaa gaatcttcta gtctccaggc
     5041 atcaaccaca aatgactcag gttacagagc aagaatttcc cagttggaaa aggaattgat
     5101 agaagatttg aagactttgc ggcacaagca ggtgatacat cctggtcttc aagaagtggg
     5161 cttaaaattg aattcagtgg atccaacaat gagcattgat cttaaatact tgggagtaca
     5221 gttacctttg gctccagcta ctagctttcc tttttggaac cttacaggaa ccaaccctgc
     5281 ctctcctgat gcgggatttc cctttgtttc taggacaggg aaaaccaatg atttcactaa
     5341 gatcaaggga tggaggggaa aatttcatag tgcttctgca tctaggaatg aaggtggaaa
     5401 ttcagaaagt tcactgaaaa atcgttctgc tttctgtagt gataagctag atgaatactt
     5461 ggaaaatgaa ggcaagctga tggaaacaag catgggtttt tcttctaatg ctcccacatc
     5521 tcctgtggtg taccagcttc ccactaagag taccagttat gtacgaacac ttgatagtgt
     5581 actaaagaag caatctacta tttccccttc tacctcttat tctttgaaac ctcattctgt
     5641 accccctgtc tctcgaaagg caaagtctca aaacagacag gcaactttca gtggccgaac
     5701 taaatcatct tataaatcca ttttaccata ccctgtttca ccaaagcaga aatactctca
     5761 tgtgattcta ggagataagg ttaccaagaa ttcttcaggc atcatctcag aaaatcaggc
     5821 gaataacttt gttgtgccaa ctttggatga aaatatattt ccaaagcaga ttagtttgcg
     5881 gcaggcacag cagcagcagc aacagcaaca gggaagtcgc cctccaggct tgtctaaatc
     5941 tcaggtgaag ctaatggacc tggaagactg tgcactttgg gaaggaaaac caaggacata
     6001 catcacagaa gagcgagcag atgtatcctt aacaactcta cttacagctc aagcatccct
     6061 caaaactaaa cctatccaca caatcataag gaaacgagcc cctccctgca acaatgactt
     6121 ctgtcgactg ggttgtgtat gttccagtct agctttggag aagcgccaac ctgctcactg
     6181 ccgccgacca gactgcatgt ttggttgtac ttgtttgaaa agaaaagttg tacttgttaa
     6241 aggaggatcc aaaactaagc attttcagag gaaggctgct catcgagatc cagtatttta
     6301 tgatactctg ggagaggagg caagggagga ggaagaagga atcagggagg aggaggaaca
     6361 attgaaagag aaaaagaaga gaaagaagct agaatacact atatgtgaga cagagcctga
     6421 acagcctgtt cgacattacc cattatgggt aaaagtagaa ggtgaagtag atccagaacc
     6481 agtttatatc cccacgcctt ctgtcattga gcctatgaaa ccattgttat tgcctcagcc
     6541 agaagtttta tctcctactg tgaagggcaa actgctcact ggaattaaat ctccacggtc
     6601 atatactccc aaacccaatc ctgtgattcg ggaagaggac aaagatccag tctacttgta
     6661 ctttgaaagt atgatgactt gtgctcgagt tcgagtatat gagcgaaaaa aagaggacca
     6721 gagacaacca tcttcctcct cctccccatc tccatcattt cagcagcaaa cttcatgtca
     6781 ttctagccct gagaaccata ataatgcaaa ggaacctgat tctgaacagc agcccttaaa
     6841 acaactcacc tgtgacttgg aggatgattc tgataaatta caagaaaaga gctggaagtc
     6901 ttcctgcaat gaaggagaat cctcttctac ttcttatatg catcagaggt cacctggtgg
     6961 tcccaccaaa ctgattgaga tcatctcaga ctgcaactgg gaggaagatc ggaacaagat
     7021 tttgagcatc ttatcccagc acatcaatag caacatgcca caatcactta aggtgggcag
     7081 cttcatcatt gagttggctt ctcagcgaaa gagccggggt gagaagaacc ctcctgttta
     7141 ttcttctcgt gtgaaaatct ctatgccatc atgtcaagac caagatgata tggctgagaa
     7201 atctggatca gagactcctg atggtccatt gtcccctggg aaaatggagg atatctctcc
     7261 tgtgcagaca gatgccctgg attcagtgag ggagagatta catggaggca aaggtctgcc
     7321 tttttatgca gggctttctc ctgcagggaa gcttgtggcc tataaacgta aacccagttc
     7381 aagtacatct gggcttatcc aggttaatgg taagagttac ccgcaggcta agttgctatt
     7441 gggacagatg ggggcattgc atccagccaa taggctagct gcttatatca caggcaggtt
     7501 gcgaccctca gtcctggacc tctcaaccct cagtactgtc atctccaagg tagcatccaa
     7561 tgccaaggtg gctgcatcca ggaaaccacg taccctgttg ccttcaacat ccaattccaa
     7621 aatggcatcc tcctctggca ctgcaacaaa tcgccctggg aagaatctga aggcgtttgt
     7681 cccagcaaaa cggccaattg cggctcgacc ctctcctggt ggtgtgttca cacagtttgt
     7741 gatgagtaaa gttggagcct tgcagcagaa gatacctgga gttagcacac cccaaaccct
     7801 ggcagggaca cagaagttca gtatcagacc ttctccagta atggtcgtca cacctgtggt
     7861 ttcttctgag ccagttcagg tgtgcagccc tgtgactgct gctgtcacta ctaccacccc
     7921 tcaagtgttt ttagaaaata ctactgctgt gacacctatg actgctattt ctgacgtgga
     7981 aactaaagaa actacttatt cttctggtgc caccactaca ggggttgttg aggtctctga
     8041 aactaatacc agcacctctg taacatctac ccagtctaca gccactgtga accttaccaa
     8101 aaccactggg ataactaccc ctgtggcttc agttgctttt cctaagtctt tggtagcatc
     8161 tccttcaacc ataactcttc ctgttgcttc cactgcttcc acctccttag tcgtggtgac
     8221 tgcagctgca tcttcctcca tggtgaccac accaacttca tctctgggct ctgttcctat
     8281 tatactctca ggaattaatg ggagtccacc agtgagccag agaccagaaa atgctgctca
     8341 aattccagtg gctactccac aggtctctcc taacacagtg aaacgtgctg gacctcgatt
     8401 gttgttgatt ccagtgcagc agggttctcc tactcttaga cctgtctcaa acacacaact
     8461 tcagggacat cggatggtct tgcagcctgt taggagtcca agtggaatga acttattcag
     8521 gcaccctaat gggcagattg tccagcttct acctttgcat cagcttcgag gctctaatac
     8581 ccagcccaac ttacagcctg tcatgtttcg gaacccaggg tctgtgatgg gaatccggtt
     8641 acctgctcct tccaaaccct ctgagactcc gccatcttcc acttcgtcct ctgctttctc
     8701 tgtcatgaat cctgtaattc aagctgttgg gtcttcttca gcagtgaatg ttatcactca
     8761 ggcaccatca ttgctttcct ctggagctag ttttgtgtct caggctggta cattgaccct
     8821 gaggatttct cctcctgaac cacaaagctt tgcaagtaaa acaggctctg aaaccaaaat
     8881 aacttatagc tcaggaggac agcctgttgg tacagccagt cttattcctc tccagtctgg
     8941 tagttttgcc ttgttacagc tcccaggaca aaagcctgtt cctagctcca ttcttcagca
     9001 tgttgcttcc cttcagatga agagagaatc tcagaatcca gaccagaaag atgaaacaaa
     9061 ctcaataaaa agagagcaag aaacgaagaa ggttctacag tcagaaggag aggctgtaga
     9121 ccctgaggct aatgtaataa aacaaaactc aggagctgct acctcagaag aaactctgaa
     9181 tgattccttg gaagataggg gtgatcattt ggatgaagaa tgccttccag aagaaggttg
     9241 tgcaactgtc aaaccatctg agcattcctg tatcactggg tcacatacag atcaagatta
     9301 taaagatgtt aatgaagaat atggggctag gaatcgtaag agttccaaag aaaaagtggc
     9361 tgttctggaa gttaggacca tttctgaaaa agccagtaat aagacagtcc aaaatttaag
     9421 taaagtacag catcaaaaac ttggtgatgt gaaggtggaa cagcagaaag gatttgacaa
     9481 tccagaagaa aactcaagtg aatttccagt cacctttaag gaagaaagta aatttgaatt
     9541 gtcaggaagc aaagttatgg agcagcaatc taatctacag ccagaggcca aagagaagga
     9601 atgtggagac tctctggaga aagacaggga aagatggaga aaacatctga agggcccctt
     9661 aaccaggaaa tgtgttggag cttcacagga atgtaagaaa gaggcagacg agcagttaat
     9721 taaagaaaca aagacatgtc aggaaaattc agatgtgttt cagcaagaac aaggcatctc
     9781 tgacttactt ggaaaaagtg gaattactga agatgccaga gttttgaaaa ctgaatgtga
     9841 ttcttggagt aggatttcta atccttcagc cttctccatt gttcctagga gagctgcaaa
     9901 aagcagcaga gggaatggac attttcaggg tcacttactg ctacctggag aacagataca
     9961 accaaagcaa gagaagaagg gtgggagaag cagtgctgac ttcactgttt tggatttgga
    10021 agaagatgat gaagatgata atgagaaaac tgatgattct attgatgaaa ttgtggatgt
    10081 tgtttctgac taccagagtg aggaggttga tgatgtagaa aagaataact gtgtagaata
    10141 cattgaggat gatgaggagc acgtggacat tgagactgta gaagagctct cagaggaaat
    10201 taatgttgct cacctgaaga ccacagcggc ccacacacag tcattcaaac agccgtcctg
    10261 tactcacatc tctgcagatg aaaaagcagc tgaaaggagt cgaaaggctc caccaattcc
    10321 tctaaaactg aagcctgatt actggagtga caaactacag aaagaagcag aagcgtttgc
    10381 ttattatcgc cggacacaca ctgccaatga gcggcggcgg cgtggtgaaa tgagggatct
    10441 ctttgagaaa ttaaagatca cattgggatt acttcattct tccaaggttt ccaaaagtct
    10501 cattcttact cgagccttca gtgaaattca gggactaaca gatcaggcag acaaattgat
    10561 aggacagaaa aatctcctga ctcgaaaacg gaatattctg atacggaaag tatcgtctct
    10621 ttcaggtaag acagaagaag tggtcctgaa gaagctagag tatatttatg caaaacagca
    10681 agcactagag gcacaaaaaa gaaaaaagaa gatgggatca gatgagtttg acatatctcc
    10741 cagaattagc aaacagcagg aaggatcttc tgcatcatct gtagatcttg gacagatgtt
    10801 tataaataac aggaggggga aacctttgat tctttccaga aaaaaagacc aggccacaga
    10861 aaatacctca cccttgaaca ctccacacac ctctgccaac cttgtgatga ctccgcaagg
    10921 gcaattgctc accctaaaag gtcccctatt ctcaggacca gtggtagctg tttctcctga
    10981 tctcttagaa tctgatctta agcctcaagt tgccggtagt gctgtggctc taccagaaaa
    11041 tgacgactta tttatgatgc cacgaattgt taatgtgaca tcattggcca cagagggagg
    11101 tttggtagat atgggtggca gcaaatatcc tcatgaagtt cctgatagca agccatctga
    11161 ccatctgaaa gacaccgtca ggaatgaaga taattcctta gaggataagg gtagaatctc
    11221 ttccagagga aacagagatg gcagagtgac gttgggtcca acgcaggttt ttctggcaaa
    11281 caaagattct ggttatccac aaatagttga cgtttccaat atgcagaaag cacaagagtt
    11341 cttacctaaa aagatttctg gtgatatgag agggattcag tataaatgga aagagagtga
    11401 atcaagaggg gagagagtga agtcaaagga ttcttcattt cataaattaa agatgaaaga
    11461 tctcaaggac tcaagcatag agatggaact gaggaaagta acatcagcta tagaggaagc
    11521 agctcttgat tccagtgaac tgctgactaa catggaagat gaggatgata ctgatgagac
    11581 actgacttca ctgctcaatg aaattgcctt tcttaatcaa caactaaatg atgactctgt
    11641 tggcctggct gaactaccca gctctatgga tacagagttc ccaggggatg ctcggcgggc
    11701 ttttattagt aaggttcctc ctggaagcag agcaactttc caggttgagc acttgggaac
    11761 tggtttgaaa gagttgcctg atgttcaagg ggagagtgac tctatcagtc ccctcctctt
    11821 gcacttggaa gacgatgact tttctgagaa tgaaaaacaa cttgcagaac cagcctctga
    11881 gccagatgtc cttaagattg ttattgactc tgaaataaag gattccctcc tttccaacaa
    11941 gaaagctatt gatggaggga agaatacttc tggcctccct gcagagcccg aaagtgtgtc
    12001 ctcacccccc accctacaca tgaagactgg cttggagaac agcaacagca cagacacttt
    12061 gtggaggcct atgccaaagt tggcccctct aggtttaaaa gtagctaatc cttccagtga
    12121 tgcagatggt cagagtctca aggtgatgcc ttgtttggca cctatagctg ccaaagttgg
    12181 gtcagttgga cacaaaatga acttaacagg gaatgaccag gaaggccggg aaagcaaggt
    12241 gatgcctaca ttggcacctg ttgtggctaa attgggcaac tcgggggcct caccaagttc
    12301 tgcagggaaa tgaacttact gggcccggga tcggccgcga ctctagatca taatcagcca
    12361 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
    12421 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    12481 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    12541 ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt
    12601 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
    12661 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
    12721 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
    12781 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
    12841 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
    12901 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
    12961 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
    13021 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    13081 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    13141 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
    13201 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    13261 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    13321 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    13381 gcccctaact ccgcccagtt ccgcc
//
