LOCUS       sequence-23021-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Hwang PM, Bunz F, Yu J, Rago C, Chan TA, Murphy MP, Kelso GF, Smith 
            RA, Kinzler KW, Vogelstein B
  TITLE     Ferredoxin reductase affects p53-dependent, 5-fluorouracil-induced 
            apoptosis in colorectal cancer cells.
  JOURNAL   Nat Med. 2001 Oct . 7(10):1111-7.
  PUBMED    11590433
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2001 Oct . 7(10):1111-7."
COMMENT     Sequence Label: FDXR KO-2A
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     413..432
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(521..717)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      535..670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcccctgag tgactatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
       61 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
      121 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
      181 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
      241 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
      301 gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
      361 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
      421 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
      481 aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc
      541 caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
      601 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
      661 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg agattgat
//
