LOCUS       sequence-23035-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Samuels Y, Wang Z, Bardelli A, Silliman N, Ptak J, Szabo S, Yan H, 
            Gazdar A, Powell SM, Riggins GJ, Willson JK, Markowitz S, Kinzler 
            KW, Vogelstein B, Velculescu VE
  TITLE     High frequency of mutations of the PIK3CA gene in human cancers.
  JOURNAL   Science. 2004 Apr 23. 304(5670):554.
  PUBMED    15016963
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Apr 23. 304(5670):554."
COMMENT     Sequence Label: PIK3CA - Exon9 E545K (pFastBac)
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             91..108
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             130..150
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ttttgtatac aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc
       61 ggatctcggt ccgaaaccat gtcgtactac catcaccatc accatcacga ttacgatatc
      121 ccaacgaccg aaaacctgta ttttcagggc gccatgggat ccatgcctcc aagaccatca
      181 tcaggtgaac tgtggggcat ccacttgatg cccccaagaa tcctagtaga atgtttacta
      241 ccaaatggaa tgatagtgac tttagaatgc ctccgtgagg ctacattaat aaccataaag
      301 catgaactat ttaaagaagc aagaaaatac cccctccatc aacttcttca agatgaatct
      361 tcttacattt tcgtaagtgt tactcaagaa gcagaaaggg aagaattttt tgatgaaaca
      421 agacgacttt gtgaccttcg gctttttcaa ccctttttaa aagtaattga accagtaggc
      481 aaccgtgaag aaaagatcct caatcgagaa attggttttg ctatcggcat gccagtgtgt
      541 gaatttgata tggttaaaga tccagaagta caggacttcc gaagaaatat tctgaacgtt
      601 tgtaaagaag ctgtggatct tagggacctc aattcacctc atagtagagc aatgtatgtc
      661 tatcctccaa atgtaaaatc ttcaccagaa ttgccaaagc acatatataa taaattagat
      721 aaagggcaa
//
