LOCUS       sequence-230443-       13631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The no force control for the F40-based human desmoplakin I tension 
            sensor serves to detect changes in FRET between YPet(short) and 
            mCherry that are tension-independent..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13631)
  AUTHORS   Price AJ, Cost AL, Ungewiss H, Waschke J, Dunn AR, Grashoff C
  TITLE     Mechanical loading of desmosomes depends on the magnitude and 
            orientation of external stress.
  JOURNAL   Nat Commun. 2018 Dec 11;9(1):5284. doi: 10.1038/s41467-018-07523-0.
  PUBMED    30538252
REFERENCE   2  (bases 1 to 13631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-11"; volume: "9(1):5284. doi"; pages: " 
            10.1038/s41467-018-07523-0"
COMMENT     Sequence Label: Desmoplakin I no force control (F40-based)
FEATURES             Location/Qualifiers
     source          1..13631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1565..2164
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1565..1584)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2061..2081
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2339..2642
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2643..2846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2797..2817
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(9089..9108)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             9599..10309
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9744..9762)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10012..10031
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             10370..10963
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(11066..11088)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    11216..11249
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    11309..11342
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     11362..11381
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11462..11479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11633..12221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11713..11732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12392..13183,13184..13252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13015..13034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13253..13357)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13425..13443
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aatgatcccc cgggctgcag gtcggccgcc
     1501 acgaccggtg ccgccaccat cccctgaccc acgcccctga cccctcacaa ggagacgacc
     1561 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc
     1621 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc
     1681 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1741 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1801 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1861 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1921 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1981 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     2041 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     2101 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     2161 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatggctc
     2221 cgaccgaagc cacccggggc ggccccgccg accccgcacc cgcccccgag gcccaccgac
     2281 tctagtaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2341 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2401 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2461 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     2521 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     2581 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     2641 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     2701 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     2761 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     2821 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
     2881 ggactcagat ctgggcccat aaagcttata cgaattcacc atgagctgca acggaggctc
     2941 ccacccgcgg atcaacactc tgggccgcat gatccgcgcc gagtctggcc cggacctgcg
     3001 ctacgaggtg accagcggcg gcgggggcac cagcaggatg tactattctc ggcgcggcgt
     3061 gatcaccgac cagaactcgg acggctactg tcaaaccggc acgatgtcca ggcaccagaa
     3121 ccagaacacc atccaggagc tgctgcagaa ctgctccgac tgcttgatgc gagcagagct
     3181 catcgtgcag cctgaattga agtatggaga tggaatacaa ctgactcgga gtcgagaatt
     3241 ggatgagtgt tttgcccagg ccaatgacca aatggaaatc ctcgacagct tgatcagaga
     3301 gatgcggcag atgggccagc cctgtgatgc ttaccagaaa aggcttcttc agctccaaga
     3361 gcaaatgcga gccctttata aagccatcag tgtcccacga gtccgcaggg ccagctccaa
     3421 gggtggtgga ggctacactt gtcagagtgg ctctggctgg gatgagttca ccaaacatgt
     3481 caccagtgaa tgtttggggt ggatgaggca gcaaagggcg gagatggaca tggtggcctg
     3541 gggtgtggac ctggcctcag tggagcagca cattaacagc caccggggca tccacaactc
     3601 catcggcgac tatcgctggc agctggacaa aatcaaagcc gacctgcgcg agaaatctgc
     3661 gatctaccag ttggaggagg agtatgaaaa cctgctgaaa gcgtcctttg agaggatgga
     3721 tcacctgcga cagctgcaga acatcattca ggccacgtcc agggagatca tgtggatcaa
     3781 tgactgcgag gaggaggagc tgctgtacga ctggagcgac aagaacacca acatcgctca
     3841 gaaacaggag gccttctcca tacgcatgag tcaactggaa gttaaagaaa aagagctcaa
     3901 taagctgaaa caagaaagtg accaacttgt cctcaatcag catccagctt cagacaaaat
     3961 tgaggcctat atggacactc tgcagacgca gtggagttgg attcttcaga tcaccaagtg
     4021 cattgatgtt catctgaaag aaaatgctgc ctactttcag ttttttgaag aggcgcagtc
     4081 tactgaagca tacctgaagg ggctccagga ctccatcagg aagaagtacc cctgcgacaa
     4141 gaacatgccc ctgcagcacc tgctggaaca gatcaaggag ctggagaaag aacgagagaa
     4201 aatccttgaa tacaagcgtc aggtgcagaa cttggtaaac aagtctaaga agattgtaca
     4261 gctgaagcct cgtaacccag actacagaag caataaaccc attattctca gagctctctg
     4321 tgactacaaa caagatcaga aaatcgtgca taagggggat gagtgtatcc tgaaggacaa
     4381 caacgagcgc agcaagtggt acgtgacggg cccgggaggc gttgacatgc ttgttccctc
     4441 tgtggggctg atcatccctc ctccgaaccc actggccgtg gacctctctt gcaagattga
     4501 gcagtactac gaagccatct tggctctgtg gaaccagctc tacatcaaca tgaagagcct
     4561 ggtgtcctgg cactactgca tgattgacat agagaagatc agggccatga caatcgccaa
     4621 gctgaaaaca atgcggcagg aagattacat gaagacgata gccgaccttg agttacatta
     4681 ccaagagttc atcagaaata gccaaggctc agagatgttt ggagatgatg acaagcggaa
     4741 aatacagtct cagttcaccg atgcccagaa gcattaccag accctggtca ttcagctccc
     4801 tggctatccc cagcaccaga cagtgaccac aactgaaatc actcatcatg gaacctgcca
     4861 agatgtcaac cataataaag taattgaaac caacagagaa aatgacaagc aagaaacatg
     4921 gatgctgatg gagctgcaga agattcgcag gcagatagag cactgcgagg gcaggatgac
     4981 tctcaaaaac ctccctctag cagaccaggg gtcttctcac cacatcacag tgaaaattaa
     5041 cgagcttaag agtgtgcaga atgattcaca agcaattgct gaggttctca accagcttaa
     5101 agatatgctt gccaacttca gaggttctga aaagtactgc tatttacaga atgaagtatt
     5161 tggactattt cagaaactgg aaaatatcaa tggtgttaca gatggctact taaatagctt
     5221 atgcacagta agggcactgc tccaggctat tctccaaaca gaagacatgt taaaggttta
     5281 tgaagccagg ctcactgagg aggaaactgt ctgcctggac ctggataaag tggaagctta
     5341 ccgctgtgga ctgaagaaaa taaaaaatga cttgaacttg aagaagtcgt tgttggccac
     5401 tatgaagaca gaactacaga aagcccagca gatccactct cagacttcac agcagtatcc
     5461 actttatgat ctggacttgg gcaagttcgg tgaaaaagtc acacagctga cagaccgctg
     5521 gcaaaggata gataaacaga tcgactttag attatgggac ctggagaaac aaatcaagca
     5581 attgaggaat tatcgtgata actatcaggc tttctgcaag tggctctatg atgctaaacg
     5641 ccgccaggat tccttagaat ccatgaaatt tggagattcc aacacagtca tgcggttttt
     5701 gaatgagcag aagaacttgc acagtgaaat atctggcaaa cgagacaaat cagaggaagt
     5761 acaaaaaatt gctgaacttt gcgccaattc aattaaggat tatgagctcc agctggcctc
     5821 atacacctca ggactggaaa ctctgctgaa catacctatc aagaggacca tgattcagtc
     5881 cccttctggg gtgattctgc aagaggctgc agatgttcat gctcggtaca ttgaactact
     5941 tacaagatct ggagactatt acaggttctt aagtgagatg ctgaagagtt tggaagatct
     6001 gaagctgaaa aataccaaga tcgaagtttt ggaagaggag ctcagactgg cccgagatgc
     6061 caactcggaa aactgtaata agaacaaatt cctggatcag aacctgcaga aataccaggc
     6121 agagtgttcc cagttcaaag cgaagcttgc gagcctggag gagctgaaga gacaggctga
     6181 gctggatggg aagtcggcta agcaaaatct agacaagtgc tacggccaaa taaaagaact
     6241 caatgagaag atcacccgac tgacttatga gattgaagat gaaaagagaa gaagaaaatc
     6301 tgtggaagac agatttgacc aacagaagaa tgactatgac caactgcaga aagcaaggca
     6361 atgtgaaaag gagaaccttg gttggcagaa attagagtct gagaaagcca tcaaggagaa
     6421 ggagtacgag attgaaaggt tgagggttct actgcaggaa gaaggcaccc ggaagagaga
     6481 atatgaaaat gagctggcaa aggtaagaaa ccactataat gaggagatga gtaatttaag
     6541 gaacaagtat gaaacagaga ttaacattac gaagaccacc atcaaggaga tatccatgca
     6601 aaaagaggat gattccaaaa atcttagaaa ccagcttgat agactttcaa gggaaaatcg
     6661 agatctgaag gatgaaattg tcaggctcaa tgacagcatc ttgcaggcca ctgagcagcg
     6721 aaggcgagct gaagaaaacg cccttcagca aaaggcctgt ggctctgaga taatgcagaa
     6781 gaagcagcat ctggagatag aactgaagca ggtcatgcag cagcgctctg aggacaatgc
     6841 ccggcacaag cagtccctgg aggaggctgc caagaccatt caggacaaaa ataaggagat
     6901 cgagagactc aaagctgagt ttcaggagga ggccaagcgc cgctgggaat atgaaaatga
     6961 actgagtaag gtaagaaaca attatgatga ggagatcatt agcttaaaaa atcagtttga
     7021 gaccgagatc aacatcacca agaccaccat ccaccagctc accatgcaga aggaagagga
     7081 taccagtggc taccgggctc agatagacaa tctcacccga gaaaacagga gcttatctga
     7141 agaaataaag aggctgaaga acactctaac ccagaccaca gagaatctca ggagggtgga
     7201 agaagacatc caacagcaaa aggccactgg ctctgaggtg tctcagagga aacagcagct
     7261 ggaggttgag ctgagacaag tcactcagat gcgaacagag gagagcgtaa gatataagca
     7321 atctcttgat gatgctgcca aaaccatcca ggataaaaac aaggagatag aaaggttaaa
     7381 acaactgatc gacaaagaaa caaatgaccg gaaatgcctg gaagatgaaa acgcgagatt
     7441 acaaagggtc cagtatgacc tgcagaaagc aaacagtagt gcgacggaga caataaacaa
     7501 actgaaggtt caggagcaag aactgacacg cctgaggatc gactatgaaa gggtttccca
     7561 ggagaggact gtgaaggacc aggatatcac gcggttccag aactctctga aagagctgca
     7621 gctgcagaag cagaaggtgg aagaggagct gaatcggctg aagaggaccg cgtcagaaga
     7681 ctcctgcaag aggaagaagc tcgaggaaga gctggaaggc atgaggaggt cgctgaagga
     7741 gcaagccatc aaaatcacca acctgaccca gcagctggag caggcatcca ttgttaagaa
     7801 gaggagtgag gatgacctcc ggcagcagag ggacgtgctg gatggccacc tgagggaaaa
     7861 gcagaggacc caggaagagc tgaggaggct ctcttctgag gtcgaggccc tgaggcggca
     7921 gttactccag gaacaggaaa gtgtcaaaca agctcacttg aggaatgagc atttccagaa
     7981 ggcgatagaa gataaaagca gaagcttaaa tgaaagcaaa atagaaattg agaggctgca
     8041 gtctctcaca gagaacctga ccaaggagca cttgatgtta gaagaagaac tgcggaacct
     8101 gaggctggag tacgatgacc tgaggagagg acgaagcgaa gcggacagtg ataaaaatgc
     8161 aaccatcttg gaactaagga gccagctgca gatcagcaac aaccggaccc tggaactgca
     8221 ggggctgatt aatgatttac agagagagag ggaaaatttg agacaggaaa ttgagaaatt
     8281 ccaaaagcag gctttagagg catctaatag gattcaggaa tcaaagaatc agtgtactca
     8341 ggtggtacag gaaagagaga gccttctggt gaaaatcaaa gtcctggagc aagacaaggc
     8401 aaggctgcag aggctggagg atgagctgaa tcgtgcaaaa tcaactctag aggcagaaac
     8461 cagggtgaaa cagcgcctgg agtgtgagaa acagcaaatt cagaatgacc tgaatcagtg
     8521 gaagactcaa tattcccgca aggaggaggc tattaggaag atagaatcgg aaagagaaaa
     8581 gagtgagaga gagaagaaca gtcttaggag tgagatcgaa agactccaag cagagatcaa
     8641 gagaattgaa gagaggtgca ggcgtaagct ggaggattct accagggaga cacagtcaca
     8701 gttagaaaca gaacgctccc gatatcagag ggagattgat aaactcagac agcgcccata
     8761 tgggtcccat cgagaggtcg agatggtgag caaaggcgaa gagctgttca ccggcgtggt
     8821 gcccatcctg gtggagctgg acggcgacgt gaacggccac aagttcagcg tgagcggcga
     8881 gggcgagggc gacgccacct acggcaagct gaccctgaag ctgctgtgca ccaccggcaa
     8941 gctgcccgtg ccctggccca ccctggtgac caccctgggc tacggcgtgc agtgcttcgc
     9001 ccggtacccc gaccacatga agcagcacga cttcttcaag agcgccatgc ccgagggcta
     9061 cgtgcaggag cggaccatct tcttcaagga cgacggcaac tacaagaccc gggccgaggt
     9121 gaagttcgag ggcgacaccc tggtgaaccg gatcgagctg aagggcatcg acttcaagga
     9181 ggacggcaac atcctgggcc acaagctgga gtacaactac aacagccaca acgtgtacat
     9241 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccggc acaacatcga
     9301 ggacggcggc gtgcagctgg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     9361 cgtgctgctg cccgacaacc actacctgag ctaccagagc gccctgttca aggaccccaa
     9421 cgagaagcgg gaccacatgg tgctgctgga gttcctgacc gccgccggat ccgggccagg
     9481 tggagcaggg ccaggtggtg cagggccagg tggtgcaggg ccaggtggtg cagggcccgg
     9541 tggtgcaggt ccaggtggtg caggtccagg tggtgctggt ccaggtggtg ctggatccat
     9601 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     9661 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     9721 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     9781 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     9841 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     9901 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     9961 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
    10021 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
    10081 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
    10141 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
    10201 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
    10261 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag gcggccgcct
    10321 atacatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
    10381 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata
    10441 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg
    10501 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
    10561 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
    10621 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
    10681 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
    10741 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
    10801 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
    10861 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
    10921 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
    10981 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg
    11041 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    11101 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    11161 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tactgataac
    11221 ttcgtataat gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa
    11281 gctagggccg cgatccggaa cccttaatat aacttcgtat aatgtatgct atacgaagtt
    11341 atcagtactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    11401 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
    11461 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    11521 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    11581 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    11641 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    11701 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    11761 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    11821 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    11881 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    11941 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    12001 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
    12061 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
    12121 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    12181 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    12241 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    12301 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    12361 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    12421 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    12481 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    12541 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    12601 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    12661 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
    12721 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    12781 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    12841 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    12901 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    12961 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
    13021 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    13081 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    13141 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    13201 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    13261 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    13321 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    13381 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    13441 ggccctttcg tcttcaagaa ttgctagcaa ttgctagcaa ttgctagcaa ttcataccag
    13501 atcaccgaaa actgtcctcc aaatgtgtcc ccctcacact cccaaattcg cgggcttctg
    13561 cctcttagac cactctaccc tattccccac actcaccgga gccaaagccg cggcccttcc
    13621 gtttctttgc t
//
