LOCUS       sequence-230453-        6295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express MLL/KMT2A promoter for luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai 
            DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G,
            Mackay JP, Kinney JB, Shi J, Vakoc CR
  TITLE     A Transcription Factor Addiction in Leukemia Imposed by the MLL 
            Promoter Sequence.
  JOURNAL   Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 
            10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29.
  PUBMED    30503706
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.10.015"; journalName: "Cancer Cell"; date: 
            "2018-12-10- 10"; volume: "34"; issue: "6"; pages: "970-981.e8"
COMMENT     Sequence Label: pGL421_MLL_promoter
FEATURES             Location/Qualifiers
     source          1..6295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1323..1442
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    1494..1615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1505..1524)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1559..1578
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1809..2166
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1809..2000
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1832..1852)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2017..2152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2079..2098
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2197..2796
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2821..2869
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(3025..3042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3196..3784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3276..3295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3984..4775,4776..4844))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4949..4997
                     /note="synthetic polyadenylation signal"
     misc_feature    5011..5102
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5051..5070
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      5959..5968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5965..6295,1..1319)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga
       61 aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca
      121 tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc
      181 atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa
      241 ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac
      301 cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga
      361 tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca
      421 ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg
      481 agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac
      541 tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg
      601 agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct
      661 tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga
      721 tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg
      781 ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt
      841 gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc
      901 tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc
      961 acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc
     1021 cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg
     1081 gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta
     1141 aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga
     1201 agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg
     1261 acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtga
     1321 attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg cccatgagct
     1381 gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc aggatcaacg
     1441 tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
     1501 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1561 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     1621 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     1681 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtttgcgt attgggcgct
     1741 cttccgctga tctgcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta
     1801 gctaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     1861 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     1921 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     1981 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     2041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     2101 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     2161 tgcaaaaagc tcgattcttc tgacactagc gccaccatga ccgagtacaa gcctaccgtg
     2221 cgcctggcca ctcgcgatga tgtgccccgc gccgtccgca ctctggccgc cgctttcgcc
     2281 gactaccccg ctacccggca caccgtggac cccgaccggc acatcgagcg tgtgacagag
     2341 ttgcaggagc tgttcctgac ccgcgtcggg ctggacatcg gcaaggtgtg ggtagccgac
     2401 gacggcgcgg ccgtggccgt gtggactacc cccgagagcg ttgaggccgg cgccgtgttc
     2461 gccgagatcg gcccccgaat ggccgagctg agcggcagcc gcctggccgc ccagcagcaa
     2521 atggagggcc tgcttgcccc ccatcgtccc aaggagcctg cctggtttct ggccactgta
     2581 ggagtgagcc ccgaccacca gggcaagggc ttgggcagcg ccgtcgtgtt gcccggcgta
     2641 gaggccgccg aacgcgccgg tgtgcccgcc tttctcgaaa caagcgcacc aagaaacctt
     2701 ccattctacg agcgcctggg cttcaccgtg accgccgatg tcgaggtgcc cgagggacct
     2761 aggacctggt gtatgacacg aaaacctggc gcctaatgat ctagaaccgg tcatggccgc
     2821 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgt tcgaactaga
     2881 tgctgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     2941 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     3001 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3061 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg
     3961 caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg
     4021 atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt
     4081 cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg
     4141 gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct
     4201 gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt
     4261 tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc
     4321 tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga
     4381 tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt
     4441 aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc
     4501 atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag
     4561 tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca
     4621 catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca
     4681 aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct
     4741 tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc
     4801 gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa
     4861 tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt
     4921 aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg
     4981 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     5041 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     5101 ctggcctaac tggccggtac ctgagctcgc tagcctcgag gatatccgag tgcacagtag
     5161 agcgaggagg tggcaggaga cctgccactc ggtgtgtgct ttttggaaag agctccagca
     5221 tgaaaatggg aggggctcct tttttatttt ttaaagtgcg ttcattgagg gcctttctgt
     5281 tttgcatata gtaaactggg cagcgcttct gttttgcatg tagtacaata agggctcttt
     5341 tattctagaa gcgttcaatt cgggctaacc catcttgtat ccgtgccctg gggcggagga
     5401 gaaggctcac tgctccccga cccagccgcc gcgttatact ggaagctgct ctcccgggct
     5461 gttcgattcc cagcgcgtcc cgggaacgtg tgtaatcggc ctctcggcgc tcaaggctgc
     5521 aggcggcccg gctccctacg cctccatcct ccgcgtagtc ctcgccccct ccccctctct
     5581 ccggaacccg cgctctgctc cgggctcccg ggcttccttc ccccctcctt ttcccgagca
     5641 atgcctctcc cggagggcgg ggcatgcagt tatccaggtt gcggggccgg cgggcgggca
     5701 ggaggcggcg gcccgggcct cgggatggag ttgcagcgcc caggcgtcag aggcggaggc
     5761 ccgaggccgc tatacagatt gcggggctgg cgggcgcggc ccggcgctct gcatagcggc
     5821 cggcagggtg caggcggccg ggcggcgcag agctggttag gcaggttccg gggccccgtg
     5881 cccccccctc cgcctccccg cccccctgtg ttgtcgcctc tccctctcga agcttggcaa
     5941 tccggtactg ttggtaaagc caccatggaa gatgccaaaa acattaagaa gggcccagcg
     6001 ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc
     6061 tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc
     6121 tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg
     6181 aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg
     6241 ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacg
//
