LOCUS       sequence-230539-        8901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized 
            AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, 
            Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, 
            Aryee MJ, Joung JK
  TITLE     Engineered CRISPR-Cas12a variants with increased activities and 
            improved targeting ranges for gene, epigenetic and base editing.
  JOURNAL   Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0.
  PUBMED    30742127
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 
            10.1038/s41587-018-0011-0."
COMMENT     Sequence Label: 
            pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA 
            (AAS1065)
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          112..1128
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1052..1074
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1136..1155
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1227..5147
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNVEKNRGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4867..4876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5148..5195
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5202..5282
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5372..5391)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5437..5492
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5491..5510)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5853..5869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5853..5869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5866..5888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5877..5893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5901..5907,5908..5925,5926..5931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5946..5967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6026..6221
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6072..6207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6227..6361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6264..6283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6318..6337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6429..6446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6600..7188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6680..6699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7359..8150,8151..8219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7982..8001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8220..8324)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8355..8734
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 rggcggrgag gkgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
       61 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
      121 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
      181 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
      241 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
      301 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
      361 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
      421 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
      481 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
      541 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
      601 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
      661 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
      721 cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga
      781 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
      841 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
      901 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
      961 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     1021 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     1081 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     1141 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tctgcagtcg acggtaccgc
     1201 gggcccggga tccaccggtc gccaccatga cacagttcga gggctttacc aacctgtatc
     1261 aggtgagcaa gacactgcgg tttgagctga tcccacaggg caagaccctg aagcacatcc
     1321 aggagcaggg cttcatcgag gaggacaagg cccgcaatga tcactacaag gagctgaagc
     1381 ccatcatcga tcggatctac aagacctatg ccgaccagtg cctgcagctg gtgcagctgg
     1441 attgggagaa cctgagcgcc gccatcgact cctatagaaa ggagaaaacc gaggagacaa
     1501 ggaacgccct gatcgaggag caggccacat atcgcaatgc catccacgac tacttcatcg
     1561 gccggacaga caacctgacc gatgccatca ataagagaca cgccgagatc tacaagggcc
     1621 tgttcaaggc cgagctgttt aatggcaagg tgctgaagca gctgggcacc gtgaccacaa
     1681 ccgagcacga gaacgccctg ctgcggagct tcgacaagtt tacaacctac ttctccggct
     1741 tttatgagaa caggaagaac gtgttcagcg ccgaggatat cagcacagcc atcccacacc
     1801 gcatcgtgca ggacaacttc cccaagttta aggagaattg tcacatcttc acacgcctga
     1861 tcaccgccgt gcccagcctg cgggagcact ttgagaacgt gaagaaggcc atcggcatct
     1921 tcgtgagcac ctccatcgag gaggtgtttt ccttcccttt ttataaccag ctgctgacac
     1981 agacccagat cgacctgtat aaccagctgc tgggaggaat ctctcgggag gcaggcaccg
     2041 agaagatcaa gggcctgaac gaggtgctga atctggccat ccagaagaat gatgagacag
     2101 cccacatcat cgcctccctg ccacacagat tcatccccct gtttaagcag atcctgtccg
     2161 ataggaacac cctgtctttc atcctggagg agtttaagag cgacgaggaa gtgatccagt
     2221 ccttctgcaa gtacaagaca ctgctgagaa acgagaacgt gctggagaca gccgaggccc
     2281 tgtttaacga gctgaacagc atcgacctga cacacatctt catcagccac aagaagctgg
     2341 agacaatcag cagcgccctg tgcgaccact gggatacact gaggaatgcc ctgtatgagc
     2401 ggagaatctc cgagctgaca ggcaagatca ccaagtctgc caaggagaag gtgcagcgca
     2461 gcctgaagca cgaggatatc aacctgcagg agatcatctc tgccgcaggc aaggagctga
     2521 gcgaggcctt caagcagaaa accagcgaga tcctgtccca cgcacacgcc gccctggatc
     2581 agccactgcc tacaaccctg aagaagcagg aggagaagga gatcctgaag tctcagctgg
     2641 acagcctgct gggcctgtac cacctgctgg actggtttgc cgtggatgag tccaacgagg
     2701 tggaccccga gttctctgcc cggctgaccg gcatcaagct ggagatggag ccttctctga
     2761 gcttctacaa caaggccaga aattatgcca ccaagaagcc ctactccgtg gagaagttca
     2821 agctgaactt tcagatgcct acactggcca gaggctggga cgtgaatgtg gagaagaaca
     2881 gaggcgccat cctgtttgtg aagaacggcc tgtactatct gggcatcatg ccaaagcaga
     2941 agggcaggta taaggccctg agcttcgagc ccacagagaa aaccagcgag ggctttgata
     3001 agatgtacta tgactacttc cctgatgccg ccaagatgat cccaaagtgc agcacccagc
     3061 tgaaggccgt gacagcccac tttcagaccc acacaacccc catcctgctg tccaacaatt
     3121 tcatcgagcc tctggagatc acaaaggaga tctacgacct gaacaatcct gagaaggagc
     3181 caaagaagtt tcagacagcc tacgccaaga aaaccggcga ccagaagggc tacagagagg
     3241 ccctgtgcaa gtggatcgac ttcacaaggg attttctgtc caagtatacc aagacaacct
     3301 ctatcgatct gtctagcctg cggccatcct ctcagtataa ggacctgggc gagtactatg
     3361 ccgagctgaa tcccctgctg taccacatca gcttccagag aatcgccgag aaggagatca
     3421 tggatgccgt ggagacaggc aagctgtacc tgttccagat ctataacaag gactttgcca
     3481 agggccacca cggcaagcct aatctgcaca cactgtattg gaccggcctg ttttctccag
     3541 agaacctggc caagacaagc atcaagctga atggccaggc cgagctgttc taccgcccta
     3601 agtccaggat gaagaggatg gcacaccggc tgggagagaa gatgctgaac aagaagctga
     3661 aggatcagaa aaccccaatc cccgacaccc tgtaccagga gctgtacgac tatgtgaatc
     3721 acagactgtc ccacgacctg tctgatgagg ccagggccct gctgcccaac gtgatcacca
     3781 aggaggtgtc tcacgagatc atcaaggata ggcgctttac cagcgacaag ttctttttcc
     3841 acgtgcctat cacactgaac tatcaggccg ccaattcccc atctaagttc aaccagaggg
     3901 tgaatgccta cctgaaggag caccccgaga cacctatcat cggcatcgat cggggcgaga
     3961 gaaacctgat ctatatcaca gtgatcgact ccaccggcaa gatcctggag cagcggagcc
     4021 tgaacaccat ccagcagttt gattaccaga agaagctgga caacagggag aaggagaggg
     4081 tggcagcaag gcaggcctgg tctgtggtgg gcacaatcaa ggatctgaag cagggctatc
     4141 tgagccaggt catccacgag atcgtggacc tgatgatcca ctaccaggcc gtggtggtgc
     4201 tggagaacct gaatttcggc tttaagagca agaggaccgg catcgccgag aaggccgtgt
     4261 accagcagtt cgagaagatg ctgatcgata agctgaattg cctggtgctg aaggactatc
     4321 cagcagagaa agtgggaggc gtgctgaacc cataccagct gacagaccag ttcacctcct
     4381 ttgccaagat gggcacccag tctggcttcc tgttttacgt gcctgcccca tatacatcta
     4441 agatcgatcc cctgaccggc ttcgtggacc ccttcgtgtg gaaaaccatc aagaatcacg
     4501 agagccgcaa gcacttcctg gagggcttcg actttctgca ctacgacgtg aaaaccggcg
     4561 acttcatcct gcactttaag atgaacagaa atctgtcctt ccagaggggc ctgcccggct
     4621 ttatgcctgc atgggatatc gtgttcgaga agaacgagac acagtttgac gccaagggca
     4681 cccctttcat cgccggcaag agaatcgtgc cagtgatcga gaatcacaga ttcaccggca
     4741 gataccggga cctgtatcct gccaacgagc tgatcgccct gctggaggag aagggcatcg
     4801 tgttcaggga tggctccaac atcctgccaa agctgctgga gaatgacgat tctcacgcca
     4861 tcgacaccat ggtggccctg atccgcagcg tgctgcagat gcggaactcc aatgccgcca
     4921 caggcgagga ctatatcaac agccccgtgc gcgatctgaa tggcgtgtgc ttcgactccc
     4981 ggtttcagaa cccagagtgg cccatggacg ccgatgccaa tggcgcctac cacatcgccc
     5041 tgaagggcca gctgctgctg aatcacctga aggagagcaa ggatctgaag ctgcagaacg
     5101 gcatctccaa tcaggactgg ctggcctaca tccaggagct gcgcaacaaa aggccggcgg
     5161 ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc ctacccatac gatgttccag
     5221 attacgctta tccctacgac gtgcctgatt atgcataccc atatgatgtc cccgactatg
     5281 cctaaagcgg ccgcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     5341 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     5401 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     5461 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     5521 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     5581 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     5641 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     5701 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     5761 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     5821 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     5881 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     5941 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6001 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
     6061 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     6121 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     6181 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
     6241 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6301 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     6361 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     6421 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     6481 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     6541 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     6601 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6661 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6721 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6781 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6841 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6901 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6961 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     7021 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     7081 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     7141 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     7201 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     7261 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     7321 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     7381 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7441 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7501 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7561 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7621 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7681 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7741 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7801 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7861 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7921 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7981 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     8041 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     8101 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     8161 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     8221 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     8281 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     8341 gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     8401 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     8461 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     8521 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     8581 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     8641 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     8701 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     8761 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     8821 attttgtgca gcgatggggg cggggggggg gggggnsgcg cgccrggsgg ggsggggsgg
     8881 ggsgrggggs ggggsggggs g
//
