LOCUS       sequence-230577-        2185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pOR1-OR2 (lambda 
            cI-repressable strong promoter) - Please see Supplemental Documents 
            for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2185)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P9m
FEATURES             Location/Qualifiers
     source          1..2185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    93..113
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(119..136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(290..878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(370..389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1049..1840,1841..1909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1672..1691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1910..2014)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2082..2100
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2104..2147)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2150..2171
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagtgctgt tccgctgggc atgctgagct aacaccgtgc gtgttgacaa ttttacctct
       61 ggcggtgata atggttgcag ctactagaga cctactagta gcggccgctg caggcttcct
      121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
      301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      721 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgc gacccacgct
     1201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1381 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1441 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1501 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1561 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1621 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1681 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1741 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1801 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1861 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1921 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1981 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     2041 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     2101 agaatttcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc
     2161 cgcttctaga gactagtggg tctca
//
