LOCUS       sequence-230580-        2267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pCin-TetO (Promoter activated
            by CinR and repressed by TetR; encodes  logic). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2267)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P15m
FEATURES             Location/Qualifiers
     source          1..2267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    113..131
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    175..195
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(201..218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(372..960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(452..471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1131..1922,1923..1991))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1754..1773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1992..2096)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2164..2182
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2186..2229)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2232..2253
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagcggaac tcttgtgcgt ggcgcatgtc gttatcgcaa aaccgctgca cacttttgcg
       61 cgacatgctc tgatccccct catctggggg ggcctatctg agggaattta cttccctatc
      121 agtgatagag ataatgagca catccctatc agtgatagag atgtactaga gacctactag
      181 tagcggccgc tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      241 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      301 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      361 ttgctggcgt ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      421 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      481 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      541 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      601 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      661 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      721 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      781 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      841 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      901 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      961 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1021 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1081 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1141 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1201 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1261 atgataccgc gcgacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1321 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1381 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1441 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1501 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1561 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1621 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1681 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1741 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1801 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1861 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1921 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1981 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2041 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2101 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2161 aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc tttcgctaag
     2221 gatgatttct ggaattcgcg gccgcttcta gagactagtg ggtctca
//
