LOCUS       sequence-230581-        2270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pCin-LacO (Promoter activated
            by CinR and repressed by LacI; encodes  logic). Please see 
            Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2270)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P16m
FEATURES             Location/Qualifiers
     source          1..2270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    144..160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    178..198
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(204..221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(375..963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(455..474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1134..1925,1926..1994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1757..1776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1995..2099)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2167..2185
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2189..2232)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2235..2256
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagcggaac tcttgtgcgt ggcgcatgtc gttatcgcaa aaccgctgca cacttttgcg
       61 cgacatgctc tgatccccct catctggggg ggcctatctg agggaattta cttgtgagcg
      121 ctcacaattg ataatgagca caattgtgag cggataacaa ttgcattact agagacctac
      181 tagtagcggc cgctgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
      241 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
      301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
      361 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
      421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
      481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
      541 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
      601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
      661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
      721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
      781 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
      841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
      901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
      961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     1021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     1081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     1141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     1201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     1261 gcaatgatac cgcgcgaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     1321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     1381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     1441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     1501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     1561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     1621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     1681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     1741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     1801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     1861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     1921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     1981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     2041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     2101 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     2161 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     2221 aaggatgatt tctggaattc gcggccgctt ctagagacta gtgggtctca
//
