LOCUS       sequence-230589-        2164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed 
            promoter, as used in DOI:10.1038/35002125). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2164)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P30m
FEATURES             Location/Qualifiers
     source          1..2164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..47
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    72..92
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(98..115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(269..857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(349..368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1028..1819,1820..1888))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1651..1670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1889..1993)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2061..2079
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2083..2126)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2129..2150
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggagataaat gtgagcggat aacattgaca ttgtgagcgg ataacaagat actgagcaca
       61 tactagagac ctactagtag cggccgctgc aggcttcctc gctcactgac tcgctgcgct
      121 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      181 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      241 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc
      301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1141 ctggccccag tgctgcaatg ataccgcgcg acccacgctc accggctcca gatttatcag
     1201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1321 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1621 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1861 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     1921 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1981 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     2041 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2101 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag actagtgggt
     2161 ctca
//
