LOCUS       sequence-231031-        2206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for mSpinach (fluorescent RNA 
            aptamer for DFHBI, suitable for in vivo use; see DOI: 
            10.1021/sb400089c). Please see Supplemental Documents for annotated 
            Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2206)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C22m
FEATURES             Location/Qualifiers
     source          1..2206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    114..134
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(140..157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(311..899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(391..410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1070..1861,1862..1930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1693..1712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1931..2035)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2103..2121
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2125..2168)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2171..2192
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 aatggacgca actgaatgaa atggtgaagg acgggtccag gtgtggctgc ttcggcagtg
       61 cagcttgttg agtagagtgt gagctccgta actagtcgcg tcaggtagag acctactagt
      121 agcggccgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      181 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      241 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      301 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      361 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      421 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      481 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      541 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      601 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      661 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      721 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      781 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      841 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
      901 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
      961 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1021 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1081 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1141 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1201 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1261 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1321 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1381 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1441 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1501 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1561 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     1621 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     1681 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     1741 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     1801 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     1861 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1921 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1981 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2041 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2101 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
     2161 atgatttctg gaattcgcgg ccgcttctag agactagtgg gtctca
//
