LOCUS       sequence-231129-        2192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for BCD13 (Medium expression 
            bi-cistronic RBS, engineered for downstream context-independence; 
            see https://doi.org/10.1038/nmeth.2404)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2192)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U3m
FEATURES             Location/Qualifiers
     source          1..2192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(156..260)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     328..346
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(350..393)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    396..417
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    531..551
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(557..574)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(728..1316)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(808..827)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1487..2192,1..86,87..155))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2110..2129
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
       61 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      121 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      181 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
      241 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
      301 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
      361 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact
      421 agtgggtctc atactgggcc caagttcact taaaaaggag atcaacaatg aaagcaattt
      481 tcgtactgaa acatcttaat catgcaatgg aggctttcta atgagagacc tactagtagc
      541 ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      721 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      781 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      841 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      901 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      961 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     1021 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     1081 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     1141 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     1201 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     1261 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     1321 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1381 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1441 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1501 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1561 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1621 taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1681 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1741 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1801 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1861 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1921 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1981 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     2041 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     2101 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     2161 gttcttcggg gcgaaaactc tcaaggatct ta
//
