LOCUS       sequence-231143-        2217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for RiboJ-UTR1 (very strong RBS, 
            prefixed by self-cleaving RiboJ for upstream context independence). 
            Please see Supplemental Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2217)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U25m
FEATURES             Location/Qualifiers
     source          1..2217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..144
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(148..191)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    194..215
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_RNA        236..285
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             315..337
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    354..374
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(380..397)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(551..1139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(631..650)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1310..2101,2102..2170))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1933..1952
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(2171..2217,1..58))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
       61 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
      121 ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta
      181 aggatgattt ctggaattcg cggccgcttc tagagactag tgggtctcat actagctgtc
      241 accggatgtg ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt
      301 ttgtttaaaa taattttgtt taactttaag aaggagatat acaatgagag acctactagt
      361 agcggccgct gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
      421 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      481 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      541 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      601 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      661 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      721 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      781 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      841 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      901 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      961 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     1021 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1081 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1141 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1201 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     1261 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1321 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1381 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1441 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1501 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1561 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1621 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1681 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1741 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1801 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     1861 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     1921 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     1981 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2041 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2101 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2161 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtc
//
