LOCUS       sequence-231704-        9441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knockdown of NF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9441)
  AUTHORS   Cui Y, Morrison H
  TITLE     Construction of cloning-friendly minigenes for mammalian expression 
            of full-length human NF1 isoforms.
  JOURNAL   Hum Mutat. 2018 Nov 8. doi: 10.1002/humu.23681.
  PUBMED    30408279
REFERENCE   2  (bases 1 to 9441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mutat. 
            2018 Nov 8. doi: 10.1002/humu.23681."
COMMENT     Sequence Label: pLV-H1-SGIPZ_NF1 sh1miR
FEATURES             Location/Qualifiers
     source          1..9441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             68..701
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    748..873
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1370..1603
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1788..1832
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2130..2247
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2312..2686)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2683..2692
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2696..2714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2706..2753)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2781..2871
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2792..2811
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     enhancer        2970..3273
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3274..3477
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3428..3448
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3474..3498
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3563..3572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3569..4267
                     /codon_start=1
                     /product="improved green fluorescent protein from 
                     Pontellina plumata (Evdokimov et al., 2006)"
                     /label=TurboGFP
                     /note="mammalian codon-optimized"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     misc_feature    4294..4866
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4461..4478)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4688..4707
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4869..5471
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     5368..5388
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5778..6366
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5831..5851)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6249..6260)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6573..6806
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6907..6924)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6913..7024
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7235..7823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7315..7334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7994..8785,8786..8854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8617..8636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8855..8959)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9015..9150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9207..9341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9244..9263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9298..9317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
       61 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
      121 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
      181 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
      241 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
      301 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
      361 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
      421 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
      481 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
      541 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      601 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      661 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
      721 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
      781 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
      841 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
      901 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
      961 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     1021 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     1081 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     1141 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     1201 gcacagcaag cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1261 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1321 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1381 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1441 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1501 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1561 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1621 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1681 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1741 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1801 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1861 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1921 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1981 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2041 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2101 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2161 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2221 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2281 tggttagtac cgggcccgct ctagtccgga atcagtcctg ctcctcggcc acgaagtgca
     2341 cgcagttgcc ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct
     2401 cggtcatggc cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg
     2461 cgtacagctc gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct
     2521 ggtcctggac cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt
     2581 cctccacgaa gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga
     2641 cgtcgcgcgc ggtgagcacc ggaacggcac tggtcaactt ggccatggtg gccctcctat
     2701 agtgagtcgt attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct
     2761 gcaggtccga ggttctagta atatttgcat gtcgctatgt gttctgggaa atcaccataa
     2821 acgtgaaatg tctttggatt tgggaatctt ataagttctg tatgagacca catcgattct
     2881 ttttttctag acgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca
     2941 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     3001 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     3061 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     3121 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     3181 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     3241 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     3301 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     3361 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     3421 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     3481 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     3541 caccgactct actagaggat ctgccaccat ggagagcgac gagagcggcc tgcccgccat
     3601 ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg
     3661 cggagagggc acccccgagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc
     3721 cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg
     3781 cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac
     3841 caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg
     3901 ctacgaggcc ggccgcgtga tcggcgactt caaggtgatg ggcaccggct tccccgagga
     3961 cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc
     4021 catgggcgat aacgatctgg atggcagctt cacccgcacc ttcagcctgc gcgacggcgg
     4081 ctactacagc tccgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat
     4141 cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggaggatc acagcaacac
     4201 cgagctgggc atcgtggagt accagcacgc cttcaagacc ccggatgcag atgccggtga
     4261 agaataatgt acaagtagcg gccgcaaatt ccgcccctct ccctcccccc cccctaacgt
     4321 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4381 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4441 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4501 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4561 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4621 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4681 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4741 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4801 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4861 ataataccat ggccaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     4921 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     4981 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5041 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5101 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggctcg cgcatggccg
     5161 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5221 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5281 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gctggggtgc
     5341 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     5401 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     5461 ccggtgcctg agtttgtttg aatgaggctt cagtacttta cagaatcgtt gcctgcacat
     5521 cttggaaaca cttgctggga ttacttcttc aggttaaccc aacagaattc tcttctgaag
     5581 aaaatatatt tctttttatt catagctctt ctcgaggtaa ccggatccag ttgtgtttta
     5641 atgtatatta atgttactaa tgtgttttat ttaaatcaat gtcagcagtg cctgctggca
     5701 gtttcaaacg taaaagattc taaaatttta cgtttgaaac tgccagcaag taaggttgac
     5761 cataacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     5821 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     5881 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5941 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6001 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6061 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6121 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     6181 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6241 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6301 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6361 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag
     6421 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6481 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6541 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
     6601 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6661 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6721 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6781 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6841 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattgtt taaacccgct
     6901 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     6961 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     7021 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     7081 agggggagga ttgggaagac aatagcaggg tatcagctca ctcaaaggcg gtaatacggt
     7141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8281 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8581 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacgaagctt
     9001 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     9061 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     9121 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     9181 tgactaactc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
     9241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9301 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     9361 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     9421 ttcatttact ctaaacctgt g
//
