LOCUS       sequence-231789-        9021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-to-T base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9021)
  AUTHORS   Huang TP, Zhao KT, Miller SM, Gaudelli NM, Oakes BL, Fellmann C, 
            Savage DF, Liu DR
  TITLE     Circularly permuted and PAM-modified Cas9 variants broaden the 
            targeting scope of base editors.
  JOURNAL   Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y.
  PUBMED    31110355
REFERENCE   2  (bases 1 to 9021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y."
COMMENT     Sequence Label: CP1300-CBEmax
FEATURES             Location/Qualifiers
     source          1..9021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        107..486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        487..690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     641..661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     732..751
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        732..750
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             800..820
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             821..1504
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             1868..3583
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5792..6040
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6071..6319
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6362..6382
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6391..6408
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6431..6448)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6437..6661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6732..6748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6732..6748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6745..6767)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6756..6772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6780..6786,6787..6804,6805..6810))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6825..6846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6963..6980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7134..7722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7214..7233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7893..8684,8685..8753))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8516..8535
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8754..8858)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8937..8956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
       61 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
      121 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      181 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      241 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      301 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      361 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      421 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      481 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      541 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      601 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      661 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagaga
      721 tccgcggccg ctaatacgac tcactatagg gagagccgcc accatgaaac ggacagccga
      781 cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc tcctcagaga ctgggcctgt
      841 cgccgtcgat ccaaccctgc gccgccggat tgaacctcac gagtttgaag tgttctttga
      901 cccccgggag ctgagaaagg agacatgcct gctgtacgag atcaactggg gaggcaggca
      961 ctccatctgg aggcacacct ctcagaacac aaataagcac gtggaggtga acttcatcga
     1021 gaagtttacc acagagcggt acttctgccc caataccaga tgtagcatca catggtttct
     1081 gagctggtcc ccttgcggag agtgtagcag ggccatcacc gagttcctgt ccagatatcc
     1141 acacgtgaca ctgtttatct acatcgccag gctgtatcac cacgcagacc caaggaatag
     1201 gcagggcctg cgcgatctga tcagctccgg cgtgaccatc cagatcatga cagagcagga
     1261 gtccggctac tgctggcgga acttcgtgaa ttattctcct agcaacgagg cccactggcc
     1321 taggtaccca cacctgtggg tgcgcctgta cgtgctggag ctgtattgca tcatcctggg
     1381 cctgccccct tgtctgaata tcctgcggag aaagcagccc cagctgacct tctttacaat
     1441 cgccctgcag tcttgtcact atcagaggct gccaccccac atcctgtggg ccacaggcct
     1501 gaagtctgga ggatctagcg gaggatcctc tggcagcgag acaccaggaa caagcgagtc
     1561 agcaacacca gagagcagtg gcggcagcag cggcggcagc aagcccatca gagagcaggc
     1621 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     1681 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
     1741 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     1801 aggcgacggc ggatcaggag gctctggcgg ttcaggtgga tcaggcggta gcggaggttc
     1861 aggtggtgac aagaagtaca gcatcggcct ggccatcggc accaactctg tgggctgggc
     1921 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     1981 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     2041 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     2101 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     2161 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     2221 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     2281 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     2341 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     2401 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     2461 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     2521 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     2581 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     2641 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     2701 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     2761 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     2821 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     2881 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     2941 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     3001 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     3061 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     3121 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     3181 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     3241 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     3301 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     3361 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     3421 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
     3481 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     3541 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     3601 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     3661 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     3721 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     3781 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     3841 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
     3901 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
     3961 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
     4021 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     4081 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     4141 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     4201 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     4261 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     4321 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     4381 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     4441 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     4501 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     4561 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     4621 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     4681 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     4741 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     4801 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     4861 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     4921 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     4981 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     5041 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     5101 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     5161 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     5221 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
     5281 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     5341 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     5401 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     5461 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
     5521 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     5581 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     5641 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     5701 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     5761 tagcggcggg agcggcggga gcggggggag cactaatctg agcgacatca ttgagaagga
     5821 gactgggaaa cagctggtca ttcaggagtc catcctgatg ctgcctgagg aggtggagga
     5881 agtgatcggc aacaagccag agtctgacat cctggtgcac accgcctacg acgagtccac
     5941 agatgagaat gtgatgctgc tgacctctga cgcccccgag tataagcctt gggccctggt
     6001 catccaggat tctaacggcg agaataagat caagatgctg agcggaggat ccggaggatc
     6061 tggaggcagc accaacctgt ctgacatcat cgagaaggag acaggcaagc agctggtcat
     6121 ccaggagagc atcctgatgc tgcccgaaga agtcgaagaa gtgatcggaa acaagcctga
     6181 gagcgatatc ctggtccata ccgcctacga cgagagtacc gacgaaaatg tgatgctgct
     6241 gacatccgac gccccagagt ataagccctg ggctctggtc atccaggatt ccaacggaga
     6301 gaacaaaatc aaaatgctgt ctggcggctc aaaaagaacc gccgacggca gcgaattcga
     6361 gcccaagaag aagaggaaag tctaaccggt catcatcacc atcaccattg agtttaaacc
     6421 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6481 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6541 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6601 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6661 gcttctgagg cggaaagaac cagctggggc tcgataccgt cgacctctag ctagagcttg
     6721 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6781 aacatacgag ccggaagcat aaagtgtaaa gcctagggtg cctaatgagt gagctaactc
     6841 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6901 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7561 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7741 tctacggggt ctgacactca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     7921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     7981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     8821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     8881 cctgacgtcg acggatcggg agatcgatct cccgatcccc tagggtcgac tctcagtaca
     8941 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     9001 gctgagtagt gcgcgagcaa a
//
