LOCUS       sequence-231800-        8943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses firefly luciferase under the control of a strong 
            ubiquitous promoter EF1a along with a WPRE cassette. Backbone is 
            compatible with both baculoviral and human production methods of 
            AAV..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Rumachik NG, Malaker SA, Poweleit N, Maynard LH, Adams CM, Leib RD, 
            Cirolia G, Thomas D, Stamnes S, Holt K, Sinn P, May AP, Paulk NK
  TITLE     Methods Matter: Standard Production Platforms for Recombinant AAV 
            Produce Chemically and Functionally Distinct Vectors.
  JOURNAL   Mol Ther Methods Clin Dev. 2020 May 22;18:98-118. doi: 
            10.1016/j.omtm.2020.05.018. eCollection 2020 Sep 11.
  PUBMED    32995354
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2020-05-22"; volume: "18:98-118. doi"; 
            pages: " 10.1016/j.omtm.2020.05.018. eCollection 2020 Sep 11"
COMMENT     Sequence Label: ssAAV-EF1a-FLuc-WPRE-HgHpA_bac_293
FEATURES             Location/Qualifiers
     source          1..8943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  183..348
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    377..511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(401..420)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     455..474
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   678..807
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        908..2086
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1139..2077
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2034..2054
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      2093..2102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2099..3751
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIRKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGETLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    3776..4364
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3829..3849)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4247..4258)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4396..4872
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4595..4614)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4912..5052
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4912..5041
                     /label=AAV2 ITR
     promoter        join(5392..5397,5398..5414,5415..5420)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5609..6142
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  6209..6433
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(6738..7326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6818..6837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7497..8288,8289..8357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8120..8139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8358..8462)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8488..8942)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8625..8646)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8836..8855
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccattcgcca ttcaggctgc aaataagcgt tgatattcag tcaattacaa acattaataa
       61 cgaagagatg acagaaaaat tttcattctg tgacagagaa aaagtagccg aagatgacgg
      121 tttgtcacat ggagttggca ggatgtttga ttaaaaacat aacaggaaga aaaatgcccc
      181 gctgtgggcg gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt
      241 atttagggaa gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa
      301 aattaaaaat gacaaaatag tttggaacta gatttcactt atctggttcg gatctcctag
      361 gcgatatcag tgatcagatc cagacatgat aagatacatt gatgagtttg gacaaaccac
      421 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      541 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      601 tatggctgat tatgatcctc tagtacttct cgacaagctt acattattga agcatttatc
      661 agggttattg tctcagacct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg
      721 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag
      781 tggccaactc catcactagg ggttcctgcg gccgcacgcg taagctttgc aaagatggat
      841 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
      901 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      961 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     1021 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     1081 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     1141 aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc
     1201 ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag
     1261 tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga
     1321 ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct
     1381 cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt
     1441 tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt
     1501 tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg
     1561 cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct
     1621 ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc
     1681 ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa
     1741 atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc
     1801 ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca
     1861 cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta
     1921 tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt
     1981 gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc
     2041 tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgaggta ccgccaccat
     2101 ggaagatgcc aaaaacatta ggaagggccc agcgccattc tacccactcg aagacgggac
     2161 cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc
     2221 ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt
     2281 tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg
     2341 cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc
     2401 tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag
     2461 ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa
     2521 gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact accagggctt
     2581 ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt
     2641 cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag
     2701 taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca
     2761 tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt
     2821 gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg
     2881 ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa
     2941 gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat
     3001 cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa
     3061 ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg
     3121 cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc
     3181 agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtgagac
     3241 actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta
     3301 cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg
     3361 cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct
     3421 gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca
     3481 ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc
     3541 cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta
     3601 tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga
     3661 ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa
     3721 ggccaagaag ggcggcaaga tcgccgtgta agaattcgat atcaagctta tcgataatca
     3781 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     3841 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3901 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3961 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     4021 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     4081 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     4141 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgccta
     4201 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     4261 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     4321 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgagcgct
     4381 gctcgagaga tctacgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg
     4441 gaagttgcca ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg
     4501 tctgactagg tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg
     4561 caagttggga agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca
     4621 gtggcacaat cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct
     4681 cagcctcccg agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt
     4741 ttttggtaga gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag
     4801 gtgatctacc caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc
     4861 ttccctgtcc ttctgatttt gtaggtaacc acgtgcggac cgagcggccg caggaacccc
     4921 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4981 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     5041 gctgcctgca ggcatgcaag ctgtagccaa ccactagaac tatagctaga gtcctgggcg
     5101 aacaaacgat gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc
     5161 accaccggca agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc
     5221 gtgcacagca ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag
     5281 accgaaacct tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc
     5341 aaggttgccg ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gttgacataa
     5401 gcctgttcgg ttcgtaaact gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     5461 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     5521 tgtttttttg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     5581 cgatgtttga tgttatggag cagcaacgat gttacgcagc agcaacgatg ttacgcagca
     5641 gggcagtcgc cctaaaacaa agttaggtgg ctcaagtatg ggcatcattc gcacatgtag
     5701 gctcggccct gaccaagtca aatccatgcg ggctgctctt gatcttttcg gtcgtgagtt
     5761 cggagacgta gccacctact cccaacatca gccggactcc gattacctcg ggaacttgct
     5821 ccgtagtaag acattcatcg cgcttgctgc cttcgaccaa gaagcggttg ttggcgctct
     5881 cgcggcttac gttctgccca agtttgagca gccgcgtagt gagatctata tctatgatct
     5941 cgcagtctcc ggcgagcacc ggaggcaggg cattgccacc gcgctcatca atctcctcaa
     6001 gcatgaggcc aacgcgcttg gtgcttatgt gatctacgtg caagcagatt acggtgacga
     6061 tcccgcagtg gctctctata caaagttggg catacgggaa gaagtgatgc actttgatat
     6121 cgacccaagt accgccacct aacaattcgt tcaagccgag atcggcttcc cggccgcgga
     6181 gttgttcggt aaattgtcac aacgccgcga atatagtctt taccatgccc ttggccacgc
     6241 ccctctttaa tacgacgggc aatttgcact tcagaaaatg aagagtttgc tttagccata
     6301 acaaaagtcc agtatgcttt ttcacagcat aactggactg atttcagttt acaactattc
     6361 tgtctagttt aagactttat tgtcatagtt tagatctatt ttgttcagtt taagacttta
     6421 ttgtccgccc acacccgctt acgcagggca tccatttatt actcaaccgt aaccgatttt
     6481 gccaggttac gcggctggtc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     6541 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8101 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8461 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     8521 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     8581 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     8641 gtccactatt aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     8701 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     8761 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     8821 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     8881 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     8941 gtc
//
