LOCUS       sequence-231822-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A-to-G base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Huang TP, Zhao KT, Miller SM, Gaudelli NM, Oakes BL, Fellmann C, 
            Savage DF, Liu DR
  TITLE     Circularly permuted and PAM-modified Cas9 variants broaden the 
            targeting scope of base editors.
  JOURNAL   Nat Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y.
  PUBMED    31110355
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 May 20. pii: 10.1038/s41587-019-0134-y. doi: 
            10.1038/s41587-019-0134-y."
COMMENT     Sequence Label: VRER-ABEmax
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        122..501
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        502..705
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     656..676
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     747..766
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        747..765
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             815..835
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2024..6124
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKEYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6167..6187
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6196..6213
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6236..6253)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6242..6466
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6537..6553)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6537..6553)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6550..6572)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6561..6577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6585..6591,6592..6609,6610..6615))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6630..6651
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6768..6785)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6939..7527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7019..7038)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7698..8489,8490..8558))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8321..8340
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8559..8663)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8742..8761)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
       61 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      121 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
      181 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      241 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      301 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      361 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      421 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      481 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      541 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      601 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      661 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
      721 cagatccgct agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat
      781 gaaacggaca gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga
      841 agtcgagttt agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg
      901 ggatgaaaga gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga
      961 gggatggaac aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact
     1021 gaggcaggga ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac
     1081 actggagcca tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt
     1141 gttcggagca cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca
     1201 ccccggcatg aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc
     1261 cctgctgagc gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca
     1321 gagctccacc gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac
     1381 aagcgagtcc gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga
     1441 gttttcccac gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgcgatga
     1501 gagggaggtg cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg
     1561 gaacagagcc atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca
     1621 gggcggcctg gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga
     1681 gccttgcgtg atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg
     1741 cgtgaggaac gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc actaccccgg
     1801 catgaatcac cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct
     1861 gtgctatttc tttcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc
     1921 caccgactcc ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga
     1981 gagcgcaaca cctgaaagca gcgggggcag cagcgggggg tcagacaaga agtacagcat
     2041 cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
     2101 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
     2161 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
     2221 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
     2281 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
     2341 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
     2401 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
     2461 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
     2521 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
     2581 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
     2641 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
     2701 ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg
     2761 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
     2821 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
     2881 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
     2941 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
     3001 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
     3061 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc
     3121 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
     3181 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
     3241 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
     3301 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
     3361 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
     3421 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc
     3481 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
     3541 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt
     3601 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc
     3661 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
     3721 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
     3781 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
     3841 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
     3901 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
     3961 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
     4021 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
     4081 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
     4141 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
     4201 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
     4261 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
     4321 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
     4381 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
     4441 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
     4501 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct
     4561 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag
     4621 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct
     4681 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg
     4741 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
     4801 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
     4861 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
     4921 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
     4981 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
     5041 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
     5101 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
     5161 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
     5221 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
     5281 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
     5341 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
     5401 ccctaagaag tacggcggct tcgtgagccc caccgtggcc tattctgtgc tggtggtggc
     5461 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
     5521 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
     5581 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
     5641 aaacggccgg aagagaatgc tggcctcagc cagagaactg cagaagggaa acgaactggc
     5701 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
     5761 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
     5821 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
     5881 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
     5941 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
     6001 tgacaccacc atcgaccgga aggagtacag aagcaccaaa gaggtgctgg acgccaccct
     6061 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
     6121 tgactctggc ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag
     6181 gaaagtctaa ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     6241 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6301 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6361 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6421 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6481 agaaccagct ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6541 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6601 agcataaagt gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6661 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6721 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6781 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6841 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6901 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6961 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     7021 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     7081 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     7141 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     7201 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     7261 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     7321 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     7381 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7441 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7501 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7561 actcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7621 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7681 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7741 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7801 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7861 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7921 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7981 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     8041 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     8101 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     8161 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     8221 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     8281 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     8341 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     8401 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8461 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8521 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8581 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8641 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     8701 tcgggagatc gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc
     8761 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga g
//
