LOCUS       sequence-232106-        8684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to express the T679A mutant form of the SOMA FRET sensor in E. 
            coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, 
            Zhang S, Krysan PJ
  TITLE     A Forster resonance energy transfer sensor for live-cell imaging of 
            mitogen-activated protein kinase activity in Arabidopsis.
  JOURNAL   Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164.
  PUBMED    30444549
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Nov 16. doi: 10.1111/tpj.14164."
COMMENT     Sequence Label: pET-SOMA-T679A
FEATURES             Location/Qualifiers
     source          1..8684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..466
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(98..117)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     308..329
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        492..596
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(597..665,666..1457)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(815..834)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1628..2216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2117..2136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2370..2387
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2402..2541
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2467..2486)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2627..2649
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2643..2834)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3647..4729)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4691..4710
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4730..4807)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5013..5032
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     5120..5139
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5120..5138
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5139..5163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5178..5200
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5208..5534
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             5556..5573
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5583..5600
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5607..5651
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             join(5664..5666,5667..5669,5670..6377)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             6813..7160
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             6909..7256
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGCGSAGGSAGGSXSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             7005..7352
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGCGSAGGSAGGSXSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSXSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             7101..7448
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGCGSAGGSAGGSXSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSXSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             7197..7544
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSXSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(7800..7802,7803..7805,7806..8318)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             8524..8541
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8594..8612)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8608..8655
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
       61 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      121 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      181 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      241 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      301 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
      361 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
      421 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
      481 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      541 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
      601 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      661 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      721 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      781 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      841 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      901 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      961 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1021 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1081 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1141 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1201 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1261 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1321 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1381 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1441 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1501 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1561 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1621 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1681 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1741 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1801 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1861 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1921 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1981 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2041 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2101 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2161 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2221 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     2281 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     2341 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     2401 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc aatggtgcac
     2461 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     2521 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2581 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2641 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     2701 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     2761 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     2821 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     2881 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     2941 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     3001 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     3061 cagcatcctg cgatgcagat ccggccggaa cataatggtg cagggcgctg acttccgcgt
     3121 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     3181 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
     3241 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
     3301 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
     3361 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
     3421 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
     3481 cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat
     3541 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
     3601 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
     3661 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3721 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     3781 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     3841 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     3901 cggtatcgtc gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa
     3961 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     4021 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     4081 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     4141 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     4201 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     4261 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     4321 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     4381 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     4441 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     4501 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     4561 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     4621 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     4681 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     4741 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     4801 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     4861 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     4921 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     4981 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     5041 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     5101 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
     5161 tcccctctag taataatttt gtttaacttt aagaaggaga tatacatatg agcgataaaa
     5221 ttattcacct gactgacgac agttttgaca cggatgtact caaagcggac ggggcgatcc
     5281 tcgtcgattt ctgggcagag tggtgcggtc cgtgcaaaat gatcgccccg attctggatg
     5341 aaatcgctga cgaatatcag ggcaaactga ccgttgcaaa actgaacatc gatcaaaacc
     5401 ctggcactgc gccgaaatat ggcatccgtg gtatcccgac tctgctgctg ttcaaaaacg
     5461 gtgaagtggc ggcaaccaaa gtgggtgcac tgtctaaagg tcagttgaaa gagttcctcg
     5521 acgctaacct ggccggttct ggttctggcc atatgcacca tcatcatcat cattcttctg
     5581 gtctggtgcc acgcggttct ggtatgaaag aaaccgctgc tgctaaattc gaacgccagc
     5641 acatggacag cccagaattc ggcatggtga gcaagggcga ggagctgttc accggggtgg
     5701 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     5761 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gcttctatgc accaccggca
     5821 agctgcccgt gccctggccc accctcgtga ccaccctggg ctacggcctg cagtgcttcg
     5881 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     5941 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     6001 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     6061 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     6121 tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgc cacaacatcg
     6181 aggacggcgg cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     6241 ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgttc aaagacccca
     6301 acgagaagcg cgatcacatg gtcctgctgg agttcctgac cgccgccggg atcactgagg
     6361 gcatgaacga gctgtacctc gagaagtttt ctcaagaaca gatcggcgaa aacattgtgt
     6421 gcagggtcat ttgtaccacg ggtcaaattc ccatccgaga tttgtcagct gatatttcac
     6481 aagtgcttaa ggaaaaacga tccataaaga aagtttggac atttggtaga aacccagcct
     6541 gtgactatca tttaggaaac atttcaagac tgtcaaataa gcatttccaa atactactag
     6601 gagaagacgg taacctttta ttgaatgaca tttccactaa tgggacctgg ttaaatgggc
     6661 aaaaagtcga gaagaacagc aatcagttac tgtcccaagg tgatgaaata accgttggtg
     6721 taggcgtgga atcagatatt ttatctctgg tcattttcat aaacgacaaa tttaagcagt
     6781 gcctggagca gaacaaagtt gatcgcggta ccagtgctgg tggtagtgct ggtggtagtg
     6841 ctggtggtag tgctggtggt agtgctggtg gttccggcag tgctggtggt agtgctggtg
     6901 gtagtaccag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg
     6961 ctggtggttc cggcagtgct ggtggtagtg ctggtggtag taccagtgct ggtggtagtg
     7021 ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggttccggc agtgctggtg
     7081 gtagtgctgg tggtagtacc agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg
     7141 ctggtggtag tgctggtggt tgyggcagtg ctggtggtag tgctggtggt agtrccagtg
     7201 ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggttccg
     7261 gcagtgctgg tggtagtgct ggtggtagtr ccagtgctgg tggtagtgct ggtggtagtg
     7321 ctggtggtag tgctggtggt agtgctggtg gttccggcag tgctggtggt agtgctggtg
     7381 gtagtaccag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg
     7441 ctggtggttc cggcagtgct ggtggtagtg ctggtggtag taccagtgct ggtggtagtg
     7501 ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggttccgga cctggttcta
     7561 agaaaatgtt ttggcggtct gcctcttggt ctgcttcacg gactgcatca caagttcctg
     7621 agggtgatga gcaaagcctg aacattccgt gtgctattag ttctgggccg agtcgaagat
     7681 gtccagctgc tcctttggca cctcgtgatc atcataacag caaggctaga gcttgtttgc
     7741 caccattgca gcctcttgcc atttctagga ggagcttaga cgagtggcct ggcggccgca
     7801 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     7861 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     7921 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     7981 tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     8041 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     8101 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     8161 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     8221 agctggagta caactacatc agcgggaacg tctatatcac cgccgacaag cagaagaacg
     8281 gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg
     8341 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     8401 acctgagcac ccagtccgcc ttaagcaaag accccaacga gaagcgcgat cacatggtcc
     8461 tgctggagtt cttgaccgcc gccgggatca ctctcggcat ggacgagctg tctagacgtc
     8521 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
     8581 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     8641 ttgaggggtt ttttgctgaa aggaggaact atatccggcc ggat
//
