LOCUS       sequence-232110-       13958 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to express the T679A mutant form of the SOMA FRET sensor with a
            nuclear localization signal in Arabidopsis thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13958)
  AUTHORS   Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, 
            Zhang S, Krysan PJ
  TITLE     A Forster resonance energy transfer sensor for live-cell imaging of 
            mitogen-activated protein kinase activity in Arabidopsis.
  JOURNAL   Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164.
  PUBMED    30444549
REFERENCE   2  (bases 1 to 13958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2018 Nov 16. doi: 10.1111/tpj.14164."
COMMENT     Sequence Label: pSOMA-T679A-NLS
FEATURES             Location/Qualifiers
     source          1..13958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(672..674,675..677,678..1385)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELY"
     CDS             1821..2168
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             1917..2264
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGXGSAGGSAGGSXSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             2013..2360
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGXGSAGGSAGGSXSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             2109..2456
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGXGSAGGSAGGSXSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             2205..2552
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
     CDS             join(2808..2810,2811..2813,2814..3326)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISGNVYITADKQKNGIK
                     ANFKIRHNIE"
     CDS             3528..3548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3573..3825
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3861..3878)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3861..3877)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3870..3892)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    4080..4104
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5405..6034
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6463..7536
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7602..7796
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8032..8054)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8140..8280
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8195..8214
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8295..8312)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8466..9054)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8546..8565)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10340..11134)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11559..11583
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    11661..11835
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11876..12901)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12968..13644)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(13011..13031)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    13835..13856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13871..13876,13877..13894,13895..13901)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13909..13925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13914..13936
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13933..13949
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13933..13949
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 attcgagctc ggtacccggg gatccttgtc gacgagtcag taataaacgg cgtcaaagtg
       61 gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac ttttcctcaa
      121 cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct caatacacgt
      181 gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct agtcgtcctc
      241 gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc ttcacaattc
      301 agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc gtgatcaagg
      361 taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt cgatcccaat
      421 ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgaagacga ttttctgggt
      481 ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca tccgatttgt
      541 tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct atctagatct
      601 ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt ttctgattaa
      661 cagaattcgg catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      721 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      781 atgccaccta cggcaagctg accctgaagc ttctatgcac caccggcaag ctgcccgtgc
      841 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
      901 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      961 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1021 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1081 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1141 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1201 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1261 ccgacaacca ctacctgagc taccagtccg ccctgttcaa agaccccaac gagaagcgcg
     1321 atcacatggt cctgctggag ttcctgaccg ccgccgggat cactgagggc atgaacgagc
     1381 tgtacctcga gaagttttct caagaacaga tcggcgaaaa cattgtgtgc agggtcattt
     1441 gtaccacggg tcaaattccc atccgagatt tgtcagctga tatttcacaa gtgcttaagg
     1501 aaaaacgatc cataaagaaa gtttggacat ttggtagaaa cccagcctgt gactatcatt
     1561 taggaaacat ttcaagactg tcaaataagc atttccaaat actactagga gaagacggta
     1621 accttttatt gaatgacatt tccactaatg ggacctggtt aaatgggcaa aaagtcgaga
     1681 agaacagcaa tcagttactg tcccaaggtg atgaaataac cgttggtgta ggcgtggaat
     1741 cagatatttt atctctggtc attttcataa acgacaaatt taagcagtgc ctggagcaga
     1801 acaaagttga tcgcggtacc agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg
     1861 ctggtggtag tgctggtggt tccggcagtg ctggtggtag tgctggtggt agtaccagtg
     1921 ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggttccg
     1981 gcagtgctgg tggtagtgct ggtggtagta ccagtgctgg tggtagtgct ggtggtagtg
     2041 ctggtggtag tgctggtggt agtgctggtg gttccggcag tgctggtggt agtgctggtg
     2101 gtagtaccag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg
     2161 ctggtggtts cggcagtgct ggtggtagtg ctggtggtag trccagtgct ggtggtagtg
     2221 ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggttccggc agtgctggtg
     2281 gtagtgctgg tggtagtacc agtgctggtg gtagtgctgg tggtagtgct ggtggtagtg
     2341 ctggtggtag tgctggtggt tccggcagtg ctggtggtag tgctggtggt agtaccagtg
     2401 ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct ggtggttccg
     2461 gcagtgctgg tggtagtgct ggtggtagta ccagtgctgg tggtagtgct ggtggtagtg
     2521 ctggtggtag tgctggtggt agtgctggtg gttccggacc tggttctaag aaaatgtttt
     2581 ggcggtctgc ctcttggtct gcttcacgga ctgcatcaca agttcctgag ggtgatgagc
     2641 aaagcctgaa cattccgtgt gctattagtt ctgggccgag tcgaagatgt ccagctgctc
     2701 ctttggcacc tcgtgatcat cataacagca aggctagagc ttgtttgcca ccattgcagc
     2761 ctcttgccat ttctaggagg agcttagacg agtggcctgg cggccgcatg gtgagcaagg
     2821 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2881 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2941 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3001 tgtcctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     3061 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3121 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3181 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3241 actacatcag cgggaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     3301 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     3361 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3421 agtccgcctt aagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttct
     3481 tgaccgccgc cgggatcact ctcggcatgg acgagctgtc tagacttcct aagaagaaga
     3541 gaaaggttgg aggatagtcg acgaatttcc ccgatcgttc aaacatttgg caataaagtt
     3601 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     3661 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     3721 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     3781 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcggaga tctgtcgacc
     3841 tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     3901 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     3961 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgctag
     4021 agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt
     4081 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     4141 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     4201 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     4261 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     4321 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     4381 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     4441 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     4501 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     4561 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     4621 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     4681 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     4741 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     4801 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     4861 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     4921 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     4981 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     5041 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     5101 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     5161 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc
     5221 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     5281 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     5341 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     5401 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     5461 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     5521 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     5581 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     5641 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     5701 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     5761 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     5821 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     5881 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     5941 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     6001 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     6061 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     6121 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     6181 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     6241 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     6301 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     6361 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg
     6421 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     6481 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     6541 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     6601 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     6661 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     6721 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     6781 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     6841 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     6901 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     6961 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     7021 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     7081 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     7141 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     7201 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     7261 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     7321 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     7381 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     7441 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     7501 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     7561 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
     7621 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     7681 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     7741 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     7801 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     7861 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     7921 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     7981 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     8041 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     8101 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     8161 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     8221 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8281 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8341 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8401 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8461 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8521 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8581 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8641 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8701 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8761 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8821 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8881 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8941 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9001 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9061 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9121 ttggtcatgc attctaggga aggtgcgaac aagtccctga tatgagatca tgtttgtcat
     9181 ctggagccat agaacagggt tcatcatgag tcatcaactt accttcgccg acagtgaatt
     9241 cagcagtaag cgccgtcaga ccagaaaaga gattttcttg tcccgcatgg agcagattct
     9301 gccatggcaa aacatggtgg aagtcatcga gccgttttac cccaaggctg gtaatggccg
     9361 gcgaccttat ccgctggaaa ccatgctacg cattcactgc atgcagcatt ggtacaacct
     9421 gagcgatggc gcgatggaag atgctctgta cgaaatcgcc tccatgcgtc tgtttgcccg
     9481 gttatccctg gatagcgcct tgccggaccg caccaccatc atgaatttcc gccacctgct
     9541 ggagcagcat caactggccc gccaattgtt caagaccatc aatcgctggc tggccgaagc
     9601 aggcgtcatg atgactcaag gcaccttggt cgatgccacc atcattgagg cacccagctc
     9661 gaccaagaac aaagagcagc aacgcgatcc ggagatgcat cagaccaaga aaggcaatca
     9721 gtggcacttt ggcatgaagg cccacattgg tgtcgatgcc aagagtggcc tgacccacag
     9781 cctggtcacc accgcggcca acgagcatga cctcaatcag ctgggtaatc tgctgcatgg
     9841 agaggagcaa tttgtctcag ccgatgccgg ctaccaaggg gcgccacagc gcgaggagct
     9901 ggccgaggtg gatgtggact ggctgatcgc cgagcgcccc ggcaaggtaa gaaccttgaa
     9961 acagcatcca cgcaagaaca aaacggccat caacatcgaa tacatgaaag ccagcatccg
    10021 ggccagggtg gagcacccat ttcgcatcat caagcgacag ttcggcttcg tgaaagccag
    10081 atacaagggg ttgctgaaaa acgataacca actggcgatg ttattcacgc tggccaacct
    10141 gtttcgggcg gaccaaatga tacgtcagtg ggagagatct cactaaaaac tggggataac
    10201 gccttaaatg gcgaagaaac ggtctaaata ggctgattca aggcatttac gggagaaaaa
    10261 atcggctcaa acatgaagaa atgaaatgac tgagtcagcc gagaagaatt tccccgctta
    10321 ttcgcacctt ccctaggtac taaaacaatt catccagtaa aatataatat tttattttct
    10381 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
    10441 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
    10501 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
    10561 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
    10621 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
    10681 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
    10741 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
    10801 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
    10861 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
    10921 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
    10981 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
    11041 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
    11101 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
    11161 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
    11221 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
    11281 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct
    11341 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
    11401 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
    11461 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
    11521 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
    11581 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt
    11641 aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt taggaattag
    11701 aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc
    11761 tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac
    11821 attattatgg agaaactcga gcttgtcgat cgacagatcc cggtcggcat ctactctatt
    11881 tctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac
    11941 agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg
    12001 ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc
    12061 cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagtc
    12121 gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac
    12181 aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca
    12241 tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg
    12301 agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca
    12361 gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt
    12421 gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga
    12481 ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg
    12541 catccatagc ctccgcgacc ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt
    12601 gcaacgtgac accctgtgaa cggcgggaga tgcaataggt caggctctcg ctaaactccc
    12661 caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac
    12721 gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta
    12781 catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc
    12841 tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca
    12901 tatctcattg ccccccggat ctgcgaaagc tcgagagaga tagatttgta gagagagact
    12961 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
    13021 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
    13081 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
    13141 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
    13201 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
    13261 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
    13321 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
    13381 gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
    13441 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
    13501 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
    13561 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
    13621 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
    13681 ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac
    13741 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    13801 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    13861 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    13921 aacaatttca cacaggaaac agctatgaca tgattacg
//
