LOCUS       sequence-232123-       13115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TetOFF:GFP:CenpA (centromere marker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13115)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 13115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1258
FEATURES             Location/Qualifiers
     source          1..13115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1436..1456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      1696..1948
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2598..3623)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4288..4306
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    4494..4512
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             join(4657..4659,4660..4662,4663..5373)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4657..4659,4660..4662,4663..5373)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4702..4723)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4963..4982)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5310..5331
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6340..6357)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6340..6356)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6349..6371)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6559..6583
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7884..8513
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8942..10015
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10081..10275
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10511..10533)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10619..10759
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10674..10693
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10774..10791)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10945..11533)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11025..11044)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11620..12414)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12839..12863
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12941..13115
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatt cagtcgcagg tacatctcat tattggattg ttgacttgac agcttggggg
       61 ttatatcgtc ggggcagatg tgacagttgg attgcacacg gccattggcg cctgccaacc
      121 atccgaccca cttttacaac cgccatactg aggaaggagg gaatgaggcg tgtcaaggca
      181 actggatcta tcgattagat agattatgtg agaaacaagc attcgtgtat caacgcgtag
      241 gcgaagctgg gagttttctc ttctttcttt ttctttcgcc ccatcaattg tagattttca
      301 cacgagaaaa ccatttcgcg gctttggtac ccggaagtga ctgctgatta accaaaagtg
      361 accccttgtc aaaatcagcg gctggtgggg gtgtcaatta acagaaacgc gctaggtcgt
      421 ggtaagtcgc gttgccagcc ccctgatttt cgcatccagc gcgtgtgccc atattgaaga
      481 tatcgattac aattacccca agcgctttgc acttgcggta ctcttttctt tgtaccattc
      541 actcaaccat ctatcctccg aattgacctt ccgtaacatc tcatttacca acaaacaaac
      601 caaacactca ctcgatctat ccgactggct tccaagaagg gcgccacata tctgcccctg
      661 tttaatttgc aattaaagag ttcccccagc tcctcccgcc ttaatcggct cgaacaagta
      721 ccacagccaa gctaccatcg agctcgctct cacatagctc aggccgagcg cccacccaac
      781 ccagaaacca tatagaaacc gacataaaga attcatggcc cgcctggata agtcaaaggt
      841 catcaactct gccttggagc tgctgaacga ggtcggcatc gagggtctca caacgaggaa
      901 gctggcccag aagctgggtg tcgagcagcc aaccctctac tggcacgtca agaacaagcg
      961 cgccctcctg gacgccctcg ccatcgagat gctggaccgc caccacaccc acttctgccc
     1021 cctggagggc gagagctggc aggacttcct gcgcaacaac gccaagagct tcaggtgcgc
     1081 cctcctgagc cacagggacg gcgccaaggt ccacctcggc acccgcccca ccgagaagca
     1141 gtacgagacc ctggagaacc agctcgcctt cctgtgccag cagggcttca gcctggagaa
     1201 cgccctctac gccctgagcg ccgtcggtca cttcaccctg ggttgcgtcc tggaggacca
     1261 ggagcaccag gtcgccaagg aagagcgcga gaccccgacc accgacagca tgcccccgct
     1321 cctgcgccag gccatcgagc tgttcgacca ccagggtgcc gagcccgcct tcctgttcgg
     1381 ccttgagctt atcatctgcg gcctggagaa gcagctgaag tgcgagagcg gctcgcccaa
     1441 gaagaagcgc aaggtcgagg gcagcgtcag catgcagcca agcctcagga gcgagtacga
     1501 gtacccggtc ttcagccacg tccaggccgg catgttcagc cccgagctgc gcaccttcac
     1561 caagggtgac gccgagcgct gggtcagcgg ctcgccggcc gacgccctgg atgatttcga
     1621 cctggatatg ctccctgccg acgccctgga cgacttcgac ctggatatgc tgtgaggcgc
     1681 ggccggccgc ccggcgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     1741 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     1801 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     1861 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     1921 cgcgcggtgt catctatgtt actagatccg atgataagct gtcaaacatg agaattgcgg
     1981 ccgcgtcgac aagaaggatt acctctaaac aagtgtacct gtgcattctg ggtaaacgac
     2041 tcataggaga gttgtaaaaa agtttcggcc ggcgtattgg gtgttacgga gcattcacta
     2101 ggcaaccatg catggttact attgtatacc catcttagta ggaatgattt tcgaggttta
     2161 tacctacgat gaatgtgtgt cctgtaggct tgagagttca aggaagaaac agtgcaatta
     2221 tctttgcgaa cccaggggct ggtgacggaa ttttcatagt caagctatca gagtaaagaa
     2281 gaggagcatg tcaaagtaca attagagaca aatatatagt cgcgtggagc caagagcgga
     2341 ttcctcagtc tcgtaggtct cttgacgacc gttgatctgc ttgatctcgt ctcccgaaaa
     2401 tgaaaataga ctctgctaag ctattcttct gcttcgccgg agcctgaagg gcgtactagg
     2461 gttgcgaggt ccaatgcatt aatgcattgc agatgagctg tatctggaag aggtaaaccc
     2521 gaaacgcatt ttattcttgt tgacatggag ctattaaatc actagaatta ctcgactggt
     2581 tccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca
     2641 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
     2701 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
     2761 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
     2821 cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag
     2881 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
     2941 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
     3001 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
     3061 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     3121 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     3181 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     3241 ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc
     3301 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg aacggcggga gatgcaatag
     3361 gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     3421 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     3481 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     3541 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     3601 gcggtgagtt caggcttttt catttggatg cttgggtaga ataggtaagt cagattgaat
     3661 ctgaaataaa gggaggaagg gcgaacttaa gaaggtatga ccgggtcgtc cacttacctt
     3721 gcttgacaaa cgcaccaagt tatcgtgcac caagcagcag atgataataa tgtcctcgtt
     3781 cctgtctgct aataagagtc acacttcgag cgccgccgct actgctacaa gtggggctga
     3841 tctgaccagt tgcctaaatg aaccatcttg tcaaacgaca caaattttgt gctcaccgcc
     3901 tggacgacta aaccaaaata ggcattcatt gttgacctcc actagctcca gccaagccca
     3961 aaaagtgctc cttcaatatc ctcgactcta gataaatcat tccactcaac attcaggctc
     4021 ctctgcgcac gtaaagtgcc aaaggcaata ccctgctcgg tggaatgccg ccgggcttgt
     4081 cgattttacg cacataggcg cattcttgac ttgaagcgga ggagttcttc gttgcgggtt
     4141 acagtgtttt aataaaagaa tggtcaaatc aaactgctag atatacctgt cagacactct
     4201 agttgttgac ccctatactc ttaatacatc agacagtaca tgcatgttgc atgatgatgt
     4261 cggtacaact cccactagac atatcctact ctatcattga tagagtgacc cttccataga
     4321 catatcctac tctatcattg atagattgac ccttccacta ggttcacttt tctctatcat
     4381 tgatagggag tggtaaacaa ttgatggaca aaatctatca atgatagaga gtgcatgtta
     4441 cctagtattg ttcttctcta tcattgatag tgactttgcc taatcactta gcaactctat
     4501 cattgataga gttctagtat tagcaaggcc tttcctcaga tcccgatcaa tccgacagcc
     4561 aaacctcatc cactctcact ttcacactct aacttatacg atcacttctc gcccgttctt
     4621 ttgaacatca aatcaactac cttacttcta tcaattatgg tgagcaaggg cgaggagctg
     4681 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     4741 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     4801 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     4861 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4921 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4981 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5041 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5101 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5161 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5221 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     5281 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5341 gggatcactc tcggcatgga cgagctgtac aagggtaccc ggggatcccc accacaaaaa
     5401 gtaaagaagg caggggccaa aaaaaccgtc ccaggcgcat caagagacag cggggtaatg
     5461 cgtacgttcc gattcattct ctcgcatcgt gtgtgtgtat atctaattcc agaccaaaac
     5521 taactccaat ccctctcttg acaaaacagc tggcgacccg atcccacaaa agaagtcgag
     5581 acgctatcgc ccgggcaccc tcgctctgcg cgagatcagg cgttaccaaa agagcaccga
     5641 cctgcttatg cgcaagcttc cctttgcccg cctggtgcgc gaggtcgcac tcagcgtccg
     5701 ccccgtcgac gagggcttca ggtggcagtc tcacgcaatc ctggccctgc aggaggccgc
     5761 cgaggccttt ttggtcaagc tgtttgagga cacgaacctg tgcgccatcc acgccaagcg
     5821 cgtcaccatc atgcaaaagg atatacagct tgccaggcgg atacgtgccg atggcatttg
     5881 agggacagat gcttcttttt ctttttctct ttcttttttt acctttgttt tcaaaaacat
     5941 gttgagatac ccaggatcgt cggtggtctt gctcgcggtg gtgtgggctt ccgtggactg
     6001 acttgattta agagtaggct acattattta cagctgcctc gcttaattgg acaagctgga
     6061 tgagcgattc tcatggtggc gcagggcgtt gtttggagtt attttttggg gtcaatgtct
     6121 aaccggtgca tacgcaatgt tgactggcgt tattggatgg ctttatattt ttttacttct
     6181 tattttctac attgtatgta atttacggct ttgatatttg gggtgtcgca tggcgatgag
     6241 ctgcggatag tttacggtat aaaacgtaat atactcatat cgtgaagtta ctctggggac
     6301 tgcttttcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg ttttacaacg
     6361 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6421 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6481 cctgaatggc gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca
     6541 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     6601 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttg gtccatttgt
     6661 atgtccatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     6721 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     6781 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     6841 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     6901 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     6961 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     7021 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     7081 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     7141 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     7201 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     7261 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     7321 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     7381 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     7441 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     7501 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     7561 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     7621 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     7681 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     7741 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     7801 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     7861 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     7921 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     7981 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     8041 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     8101 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     8161 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     8221 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     8281 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     8341 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     8401 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     8461 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     8521 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     8581 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     8641 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     8701 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     8761 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     8821 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     8881 gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     8941 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     9001 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     9061 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     9121 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     9181 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     9241 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     9301 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     9361 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     9421 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     9481 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     9541 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     9601 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     9661 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     9721 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     9781 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     9841 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     9901 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     9961 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
    10021 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
    10081 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
    10141 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
    10201 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
    10261 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
    10321 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
    10381 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
    10441 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
    10501 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    10561 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
    10621 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
    10681 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10741 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
    10801 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
    10861 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
    10921 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
    10981 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    11041 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    11101 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    11161 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    11221 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    11281 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    11341 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
    11401 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    11461 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    11521 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    11581 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
    11641 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
    11701 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
    11761 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
    11821 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
    11881 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
    11941 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
    12001 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
    12061 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
    12121 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
    12181 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
    12241 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
    12301 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
    12361 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
    12421 tttccttcct cttttctata gtatttaaag ataccccaag aagctaatta taacaagacg
    12481 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
    12541 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
    12601 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
    12661 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
    12721 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
    12781 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
    12841 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
    12901 cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
    12961 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
    13021 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
    13081 tatctgggaa ctactcacac attattatgg agaaa
//
