LOCUS       sequence-232156-       11478 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rfp ""test"" strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan 
            YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, 
            Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
  TITLE     Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
  JOURNAL   Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z.
  PUBMED    30617347
REFERENCE   2  (bases 1 to 11478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 
            10.1038/s41564-018-0327-z."
COMMENT     Sequence Label: pJMP1069
FEATURES             Location/Qualifiers
     source          1..11478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(58..849,850..918))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     681..700
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(919..1023)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1091..1109
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1147..1169)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1269..1288
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1702..1718
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1703..1719
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      1747..1841
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      1944..2030
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    2081..2128
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(2196..2432)
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     promoter        complement(join(2758..2763,2764..2780,2781..2786))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    2877..2924
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(3062..3739)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             3746..3757
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(4087..4093,4094..4110,4111..4116))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     CDS             complement(4538..5416)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        5443..5727
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     RBS             5754..5776
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(5788..5814,5815..5817,5818..8304,8305..8307,
                     8308..9891)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9901..9990
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     mobile_element  10146..10311
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            10470..10578
                     /note="incP origin of transfer"
     rep_origin      10847..11235
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
       61 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      121 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      181 tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag caataaacca
      241 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
      301 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
      361 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
      421 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
      481 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
      541 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
      601 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
      661 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
      721 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
      781 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
      841 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
      901 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
      961 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1021 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     1081 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgacgacgg
     1141 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     1201 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     1261 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     1321 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     1381 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctag aggaccagcc
     1441 gcgtaacctg gcaaaatcgg ttacggttga gtaataaatg gatgccctgc gtaagcaggt
     1501 gtgggcggac aataaagtct taaactgaac aaaatagatc taaactatga caataaagtc
     1561 ttaaactaga cagaatagtt gtaaactgaa atcagtccag ttatgctgtg aaaaagcata
     1621 ctggactttt gttatggcta aagcaaactc ttcattttct gaagtgcaaa ttgcccgtcg
     1681 tattaaagag gggcgtgggg ttcgaggtcg acggtatcga taagctagct taattagctg
     1741 agcttggact cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca
     1801 gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa tccaagctag actgcgatga
     1861 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggggt
     1921 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1981 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgctag
     2041 gagcttgcgg cccggacgat gagctcgaat tggggatctt gaagtaccta ttccgaagtt
     2101 cctattctct agaaagtata ggaacttcag agcgcttttg aagctgatgc tcgagtgctt
     2161 aaaaacttac tcaatggaat aattctagat aattcttagg ccacacgttc aagtgcagcc
     2221 acaggataaa tttgcactga gcctgggtgg gattcggact cgaccgcata gccttcagga
     2281 gtgagttttg tgcaatacca accgacgact tgaccctgcc aagcggcacc agatttcttg
     2341 cgtacgcgat cccctaagcc aaaggtggca ctcaggggaa gcgcaaactg ccctgcaacg
     2401 ggagcgttgg cttcatcgct actttgaccc atgtcgaatc cttcttgtga atctattatg
     2461 gcgacaagca aatcgagctc tgactgccta ccccacaaca actatcagaa agcaccagca
     2521 caacggctgc ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     2581 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     2641 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     2701 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     2761 ttacgaaccg aacaggctta tgtcaactgg gttcgtgaat tatccattgc tgttgacaaa
     2821 gggaatcagg ctcgagagtt caacagacag ccttcgttct tatggccgtt caggaggaag
     2881 ttcctattcc gaagttccta ttctctagaa agtataggaa cttcttgaga agagaaaaga
     2941 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa
     3001 gttttttggc tgaaacgaat gacgaaaagg ctgtctgagc aaatccagga ggtcgttttt
     3061 attaagcacc ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt
     3121 cttcgttgtg ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa
     3181 ccggtttttt agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca
     3241 gtttcagacg cattttgatt tcacctttca gagcaccgtc ttccgggtac atacgttcgg
     3301 tggaagcttc ccaacccatg gtttttttct gcataaccgg accgtcggac gggaagttgg
     3361 taccacgcag tttaactttg tagatgaact caccgtcttg cagggaggag tcctgggtaa
     3421 cggtaacaac accaccgtct tcgaagttca taacacgttc ccatttgaaa ccttccggga
     3481 aggacagttt caggtagtcc gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt
     3541 actggaactg cggggacagg atgtcccaag cgaacggcag cggaccacct ttggtaactt
     3601 tcagtttagc ggtctgggta ccttcgtacg gacgaccttc accttcacct tcgatttcga
     3661 actcgtgacc gttaacggaa ccttccatac gaactttgaa acgcatgaac tctttgataa
     3721 cgtcttcgct actcgccatg gtacctttct cctctttaat gaattcagat ctattatacc
     3781 taggactgag ctagctgtca aattcaccac cctgaattga ctctcaaacc gtattagccc
     3841 ggtattttgg aaatagcgga agcactgact tttgttatca ataaaaaagg ccccccgtta
     3901 gggaggcctt attgttcgtc tactcggaag agcgagagac aacagaacgg tcagccacat
     3961 gaattcaaaa aaaaagcacc gactcggtgc cactttttca agttgataac ggactagcct
     4021 tatttaaact tgctatgctg tttccagcat agctcttaaa cagaccgcta aactgaaagt
     4081 tccacacatt atacgagccg gatgattaat tgtcaacagc tcatttcaga atatttgcca
     4141 gaaccggaat tctttcagct cagtcgatag gtagtaggca agagtagtcg cacctttggt
     4201 cgaaaaaaaa agcccgcact gtcaggtgcg ggcttttttc tgtgtttccc caaaagtaaa
     4261 aacccgccga agcgggtttt tacgtaaaac aggtgaaact gaccagacga gaaggctttg
     4321 gaggtgataa tggggctcaa ggacccggga ggcagatagg taggcatggc ccccattttc
     4381 aatacaagca acgcatgaga aagcccccgg aagatcacct tccgggggct tttttattgc
     4441 gctccggcaa ttaaaaaagc ggctaaccac gccgcttttt ttacgtctgc agagctcata
     4501 ggcaagcgaa tcgtgatgcc tcttagccag tactagttta tgacaacttg acggctacat
     4561 cattcacttt ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt
     4621 taaatacccg cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga
     4681 taggcatccg ggtggtgctc aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc
     4741 agcttaagac gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca
     4801 agcaaacatg ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga
     4861 tgtactgaca agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg
     4921 cttccatgcg ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc
     4981 gcccttcccc ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt
     5041 gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat tgacggccag ttaagccatt
     5101 catgccagta ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg
     5161 gatgacgacc gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc
     5221 aaacaaattc tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa
     5281 tataaccttt cattcccagc ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa
     5341 tcggcgttaa acccgccacc agatgggcat taaacgagta tcccggcagc aggggatcat
     5401 tttgcgcttc agccatactt ttcatactcc cgccattcag agaagaaacc aattgtccat
     5461 attgcatcag acattgccgt cactgcgtct tttactggct cttctcgcta accaaaccgg
     5521 taaccccgct tattaaaagc attctgtaac aaagcgggac caaagccatg acaaaaacgc
     5581 gtaacaaaag tgtctataat cacggcagaa aagtccacat tgattatttg cacggcgtca
     5641 cactttgcta tgcaatagca tttttatcca taagattagc ggatcctacc tgacgctttt
     5701 tatcgcaact ctctactgtt tctccatacc cgtttttttg ggctagaaat aattttgttt
     5761 aactttaaga aggagatata catacccatg gataagaaat actcaatagg cttagctatc
     5821 ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc gtctaaaaag
     5881 ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat aggggctctt
     5941 ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc tcgtagaagg
     6001 tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa tgagatggcg
     6061 aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga agaagacaag
     6121 aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta tcatgagaaa
     6181 tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa agcggatttg
     6241 cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt tttgattgag
     6301 ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt ggtacaaacc
     6361 tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc taaagcgatt
     6421 ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca gctccccggt
     6481 gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt gacccctaat
     6541 tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa agatacttac
     6601 gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga tttgtttttg
     6661 gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt aaatactgaa
     6721 ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca tcatcaagac
     6781 ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa agaaatcttt
     6841 tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag ccaagaagaa
     6901 ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga attattggtg
     6961 aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg ctctattccc
     7021 catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga cttttatcca
     7081 tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat cccttattat
     7141 gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa gtctgaagaa
     7201 acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc tcaatcattt
     7261 attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact accaaaacat
     7321 agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa atatgttact
     7381 gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat tgttgattta
     7441 ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta tttcaaaaaa
     7501 atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta
     7561 ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa
     7621 aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg
     7681 attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt
     7741 aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg
     7801 gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc
     7861 aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca
     7921 caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct
     7981 gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg
     8041 gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa
     8101 aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta
     8161 ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc
     8221 tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat
     8281 cgtttaagtg attatgatgt cgatgccatt gttccacaaa gtttccttaa agacgattca
     8341 atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca
     8401 agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta
     8461 atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt
     8521 gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg
     8581 gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga
     8641 gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa
     8701 ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc
     8761 gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt
     8821 gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa
     8881 gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca
     8941 cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa
     9001 attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa
     9061 gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga gtcaatttta
     9121 ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc aaaaaaatat
     9181 ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa ggtggaaaaa
     9241 gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat tatggaaaga
     9301 agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa ggaagttaaa
     9361 aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa cggtcgtaaa
     9421 cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct gccaagcaaa
     9481 tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag tccagaagat
     9541 aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga gattattgag
     9601 caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga taaagttctt
     9661 agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa tattattcat
     9721 ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga tacaacaatt
     9781 gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat ccatcaatcc
     9841 atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga cgcggccgcg
     9901 gagcagaaac tcatctctga agaagatctg gaacaaaagt tgatttcaga agaagatctg
     9961 gaacagaagc tcatctctga ggaagatctg taataaggcg cgcctcctta atgggacttg
    10021 cagcctcggt accaaattcc agaaaagaca cccgaaaggg tgttttttcg ttttggtccc
    10081 acagaatgag catcatggca ctagtcgaca tcggagaaga gtacggctct tttaaccgcc
    10141 tcaagaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat
    10201 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
    10261 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac agcggggcat
    10321 ttttcttcct gttatgtttg ggcgctgcat taatgaatcg gccaacgcgc ggggagaggc
    10381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg acccgctgcg ctcggtcgtt
    10441 cggctgcggc gagcggtatc agagcttatc ggccagcctc gcagagcagg attcccgttg
    10501 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
    10561 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
    10621 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
    10681 atgaccccga agcagggtta tgcagcggaa agtatacctt aaggaatccc ctgataacgc
    10741 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcact
    10801 ccctgcctct gtcatcacga tactgtgatg ccatggctaa ttcccatgtc agccgttaag
    10861 tgttcctgtg tcactcaaaa ttgctttgag aggctctaag ggcttctcag tgcgttacat
    10921 ccctggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt atatattctt
    10981 ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt atattaattt
    11041 tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg ttagccatga
    11101 gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt agtacgttaa
    11161 acatgagagc ttagtacgtg aaacatgaga gcttagtacg tactatcaac aggttgaact
    11221 gctgatcttc agatcctcta cgccggacgc atcgtggccg gatcttgcgg ccgcaaaaat
    11281 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11341 caatgcttaa tcagtgaggc accaataact gcctttgatc ttttctacgg ggtctgacgc
    11401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
    11461 cacctagatc cttttaaa
//
