LOCUS       sequence-232238-       10048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Alp6:Bar:mCherry (SPB/MTOC marker).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10048)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 10048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1344
FEATURES             Location/Qualifiers
     source          1..10048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1021..1566
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="SPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQEW
                     TDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGST
                     LYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGFW
                     QLDFSLPVPPRPVLPVTEI"
     CDS             1567..2274
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     1977..1996
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2276..2292)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2276..2292)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3273..3290)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3273..3289)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3282..3304)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    3492..3516
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4817..5446
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5875..6948
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7014..7208
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7444..7466)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7552..7692
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7607..7626
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7707..7724)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7878..8466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7958..7977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8553..9347)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9772..9796
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9874..10048
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ctcgagaatt cgcaatatgc aagcccagaa gtgctgcggc gcctggacgc tcgcatgcta
       61 cagctttcgc aaggcgacat tggctgggat ttgttcacac tggagtacaa gatcgacgct
      121 ccggttgact gcatcctgac agagtggcac ggtaggcaat acctgaaaat gttcaacttc
      181 ctctggcgcg tcaagcgcgt cgaatttgct ctacactcgg tatggcggaa gagcaccaca
      241 ggctcacgcg gtgtgctgca aactaaggat tcgaccgtcg cccagacgtg gcggaccacg
      301 cgcggaaccc tggccgaaat ggtccattgg actggccagc tgcagtacta catcctgttt
      361 gaggttatcg aagcctcctg gggccagctc cagaaggcac tgaaaaaaga ggatgccacg
      421 ctcgacgatc taatcgacgc ccacaccacc tacctggaca ctatcacgca aaagggcctt
      481 ctcggcgcca agcgcaaggc tacaacgaat gcccccgctg acgcaggcgc ggcggacgag
      541 gagcgctctg tacccatgaa ccagatcgcc gacatcttgc gcaacatcct atcttatcgc
      601 gacagcgtcg acggcctgta ctcctgggcc gtgtctgact tcacctcgcg gcagaacgat
      661 gccatcacag ccgctgccgc tcatgaggac accgagatgg gtggcaccgc ggccagcagc
      721 aataacccac tgggatttgc ctctgccgct acctccgagt tccctgcctt gcaggagcgt
      781 ctgcgcgttc taggcgccga cttccggaag cgtgttcaga ggctgctggg cgacctagca
      841 tacacctcgg agcatgagac gcgcttccta ggcctggcca tgaatttcaa cgacgtctac
      901 cagcctatgc tcagggccag acacaagcct ggtggcagtg gcagggcttc tgctgccgct
      961 gctgcctcgg ccgtgtccgt tccggggtct gccaggggcg agcgtgctgg agacggtacc
     1021 agcccagaac gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc
     1081 tgcaccatcg tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag
     1141 gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc
     1201 gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg gtccctggaa ggcacgcaac
     1261 gcctacgact ggacggccga atcgaccgtg tacgtctccc cccgccacca gcggacggga
     1321 ctgggctcca cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc
     1381 gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga
     1441 tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg
     1501 ggtttctggc agctggactt cagcctgccg gttccgcccc gtccggtcct gcccgtcacc
     1561 gagattgtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     1621 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     1681 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     1741 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     1801 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     1861 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     1921 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     1981 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     2041 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     2101 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     2161 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     2221 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaggatcc
     2281 actagttcta gataagtagt ttgagaagga atgacgctct ctttggctct tcgacaacag
     2341 ggtaagatac tggagtatgt ggtcccatcc ttattgcgtt gttgggatgg gcctttggat
     2401 tgacgatgat gttgccgcaa tagcgtcaca taatgacatt ccaacttgta cggatgatgc
     2461 tgagtcgaca acggttttgt tggttggctt ttagtaaaga aattgcccag aggggcctgg
     2521 ttgtcccgtt tgatctatct gcagtatttt gcaacgactg tgcctagtat gtcgttagat
     2581 accccgatat agccaatgaa ttgtgatgca tatatccagt acttcaaatt gccgtgggtg
     2641 gcatagttta cacttggatc agttttgcat tcatcacaaa gcgaagtagc aggcaaattt
     2701 tttattccaa atagatttgc tcttgtcgta ttggtagata tcgtagcttt aactcatcga
     2761 tcgtcccttc gtacaagtca taatgtggta aggcaagccc atccatccca caccaagtgc
     2821 gtcttgccca aggatgacga tggagtagcc cgccaatatg aatgttatga tgcgtatcca
     2881 tcatggagag gtgcagtgaa gattgcgtcc acgccctgtc ccacaaaaga aaggagaaaa
     2941 caaaaacagc tgatcaagca gagaagtcaa aaataaaatg tggagaaagc aactggagat
     3001 tccatgtgat gagacgagca atcatgagaa aaacaggtgc ttacaccgcc cgacaccaaa
     3061 tcgtcaaagg tgttcaaggt tcaaaagtag cagaccaacc catcccggaa tttgttacac
     3121 gatacacctc ccaaataatt ccaatgcgat cccttccggg tatagataaa aacaagggga
     3181 atatcaaatg accaccagac tccaacgccg tcaaaagaga tggggggctt cgaatatgat
     3241 acaacccaac cactcatcga cccaagcttg gcactggccg tcgttttaca acgtcgtgac
     3301 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3361 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3421 ggcgaatgct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa
     3481 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     3541 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttggtccatt tgtatgtcca
     3601 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     3661 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     3721 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     3781 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     3841 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     3901 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3961 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     4021 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     4081 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     4141 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     4201 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     4261 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     4321 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     4381 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     4441 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     4501 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     4561 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     4621 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     4681 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     4741 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     4801 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     4861 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     4921 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4981 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     5041 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     5101 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     5161 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     5221 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     5281 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     5341 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     5401 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     5461 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     5521 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     5581 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     5641 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     5701 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     5761 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     5821 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     5881 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     5941 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     6001 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     6061 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     6121 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     6181 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     6241 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     6301 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     6361 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     6421 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     6481 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     6541 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     6601 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     6661 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     6721 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     6781 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     6841 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     6901 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     6961 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     7021 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     7081 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
     7141 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
     7201 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
     7261 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
     7321 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
     7381 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
     7441 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     7501 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     7561 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     7621 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     7681 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7741 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7801 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7861 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7921 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7981 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8041 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8101 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8161 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8221 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8281 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8341 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8401 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8461 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8521 cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa
     8581 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
     8641 tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg
     8701 tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag
     8761 atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc
     8821 tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg
     8881 caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct
     8941 aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc
     9001 gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg
     9061 acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg
     9121 acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca
     9181 tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct
     9241 cccaccagct tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat
     9301 ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt
     9361 cctcttttct atagtattta aagatacccc aagaagctaa ttataacaag acgaactcca
     9421 attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt
     9481 tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac
     9541 aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg
     9601 tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt
     9661 ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc
     9721 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     9781 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     9841 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
     9901 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     9961 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
    10021 gaactactca cacattatta tggagaaa
//
